Isolation of cancer stem cells by the staining nucleic acid and analyses of their molecular specificities

• Project/Area Number: 24590688
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: TAKESHITA Akihiro 浜松医科大学, 医学部附属病院, 准教授 (00242769)
• Co-Investigator(Kenkyū-buntansha): OHNISHI Kazuknori 浜松医科大学, 医学部付属病院, 教授 (80252170)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 核酸 / がん幹細胞 / 蛍光染色 / 細胞分離 / 蛍光標識

Outline of Final Research Achievements

It is important to analyze chemotherapy-resistant cells after the treatment of anti-cancer drugs, because it might be useful to monitor the depth of remission and detect earlier relapse. We tried to analyze these cells alive by molecular methods after we get alive chemotherapy-resistant cells. The probes against bcr-abl fusion RNA was biotinized and then labelled fluorescences. We analyzed the fluorescence after energy transfer after insertion of the probes and incubation. The intensity of fluorescence was increased compared with bcr-abl negative cells. However it was not enough to separate them by flow cytometry. Another effort will be needed to increase the sensitivity.

Research Products

• [Journal Article] Expression of CD56 is an unfavorable prognostic factor for acute promyelocytic leukemia with higher initial white blood cell counts. (2014)
  • Author(s): Ono T, Takeshita A, Kishimoto Y, Kiyoi H, Okada M, Yamauchi T, Emi N, Horikawa K, Matsuda M, Shinagawa K, Monma F, Ohtake S, Nakaseko C, Takahashi M, Kimura Y, Iwanaga M, Asou N, Naoe T; Japan Adult Leukemia Study Group.
  • Journal Title: Cancer Sci Volume: 105 Issue: 1 Pages: 97-104
  • DOI: 10.1111/cas.12319
  • Peer Reviewed / Open Access
• [Journal Article] Tamibarotene for the treatment of acute promyelocytic leukemia. (2014)
  • Author(s): Takeshita A, Shinagawa K, Adachi M, Ono T, Kiguchi T, Naoe T.
  • Journal Title: Exp Opin Orphan Drugs Volume: 2(9) Pages: 961-9
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Comprehensive analysis of genetic alterations and their prognostic impacts in adult acute myeloid leukemia patients (2014)
  • Author(s): Kihara R, S. Ogawa, S. (32/33) Naoe, T.
  • Journal Title: Leukemia Volume: inpress Issue: 8 Pages: 1586-95
  • DOI: 10.1038/leu.2014.55
  • Peer Reviewed
• [Journal Article] Role of hematopoietic stem cell transplantation for relapsed acute promyelocytic leukemia: a retrospective analysis of JALSG-APL97. (2013)
  • Author(s): Fujita H, Asou N, Iwanaga M, Hyo R, Nomura S, Kiyoi H, Okada M, Inaguma Y, Matsuda M, Yamauchi T, Ohtake S, Izumi T, Nakaseko C, Ishigatsubo Y, Shinagawa K, Takeshita A, Miyazaki Y, Ohnishi K, Miyawaki S, Naoe T; Japan Adult Leukemia Study Group.
  • Journal Title: Cancer Sci Volume: 104 Issue: 10 Pages: 1339-1345
  • DOI: 10.1111/cas.12230
  • Peer Reviewed
• [Journal Article] Efficacy and resistance of gemtuzumab ozogamicin for acute myeloid leukemia. (2013)
  • Author(s): Takeshita A
  • Journal Title: Int J Hematol. Volume: 97(6) Issue: 6 Pages: 703-16
  • DOI: 10.1007/s12185-013-1365-1
  • NAID: 10031183726
  • Peer Reviewed
• [Journal Article] Phase 2 study of arsenic trioxide followed by autologous hematopoietic cell transplantation for relapsed acute promyelocytic leukemia. (2013)
  • Author(s): Yanada M, Tsuzuki M, Fujita H, Fujimaki K, Fujisawa S, Sunami K, Taniwaki M, Ohwada A, Tsuboi K, Maeda A, Takeshita A, Ohtake S, Miyazaki Y, Atsuta Y, Kobayashi Y, Naoe T, Emi N.
  • Journal Title: Blood Volume: 121 Issue: 16 Pages: 3095-3012
  • DOI: 10.1182/blood-2012-11-466862
  • Peer Reviewed
• [Journal Article] Education in transfusion medicine for medical students and doctors. (2013)
  • Author(s): Panzer S, Takeshita A, et al.
  • Journal Title: Vox Sang Volume: 104 Issue: 3 Pages: 250-272
  • DOI: 10.1111/j.1423-0410.2012.1661.x
  • Peer Reviewed
• [Journal Article] Efficacy and resistance of gemtuzumab ozogamicin for acute myeloid leukemia (2013)
  • Author(s): Takeshita A
  • Journal Title: Int J Hematology Volume: 97
  • NAID: 10031183726
  • Peer Reviewed
• [Journal Article] Long-term outcome and prognostic factors of elderly patients with acute promyelocytic leukemia (2012)
  • Author(s): Ono T, Takeshita A, Kishimoto Y, Kiyoi H, Okada M, Yamauchi T, Tsuzuki M, Horikawa K, Matsuda M, Shinagawa K, Monma F, Ohtake S, Nakaseko C, Takahashi M, Kimura Y, Iwanaga M, Asou N, Naoe T
  • Journal Title: Cancer Sci. Volume: 103 Issue: 11 Pages: 174-1978
  • DOI: 10.1111/j.1349-7006.2012.02390.x
  • Peer Reviewed
• [Presentation] Alloimmunity to Erythrocytes in Patients during Pregnancy in South Korea and Japan; Recent Results from a Cooperative International Study of Alloimmunity to Antigen Diversity in Asian Populations. (2014)
  • Author(s): Adachi M, Takeshita A, Kim DW, Han KS, Kwon SY, Kim HO, Suh JS, Watanabe H, Uchikawa M, Kino S, Ohto H.
  • Organizer: Annual Meeting of the American Society of Hematology
  • Place of Presentation: San Francisco
  • Year and Date: 2014-12-06 – 2014-12-10
• [Presentation] Differences in Transfusion-Related Alloimmunity to Erythrocytes Between South Korea and Japan; Recent Results from the Third Cooperative International Study of Alloimmunity to Antigen Diversity in Asian Populations. (2014)
  • Author(s): Takeshita A, Adachi M, Kim DW, Han KS, Kwon SY, Kim HO, Suh JS, Watanabe H, Uchikawa M, Tomoda Y, Yurugi K, Kino S, Ohto H.
  • Organizer: Annual Meeting of the American Society of Hematology
  • Place of Presentation: San Francisco
  • Year and Date: 2014-12-06 – 2014-12-10
• [Presentation] Prognostic Impact of Chromosomal Variation in Patients with Acute Promyelocytic Leukemia (APL); Analysis of 775 Cases Enrolled in the Japan Adult Leukemia Study Group APL Studies. (2014)
  • Author(s): Adachi M, Takeshita A, Taki T, Ohtake S, Shinagawa K, Kiyoi H, Matsuda M, Takahashi M, Emi N, Kobayashi Y, Miyamura K, Fujita H, Sakura T, Iwanaga M, Usui N, Miyawaki S, Asou N, Ohnishi K, Miyazaki Y, Naoe T.
  • Organizer: Annual Meeting of the American Society of Hematology
  • Place of Presentation: San Francisco
  • Year and Date: 2014-12-06 – 2014-12-10
• [Presentation] Collaborative study on irregular erythrocyte alloimmunity in Japan; Recent results from Japanese Study Group of Antigen Diversity in Asian Populations (allo-ADP) Study Group. (2014)
  • Author(s): Watanabe H, Takeshita A, Adachi M, Yamada C, Yurugi K, Tomoda Y, Uchikawa M, Kino S, Ohto H.
  • Organizer: International Society of Blood Transfusion
  • Place of Presentation: Soul
  • Year and Date: 2014-06-01 – 2014-06-04
• [Presentation] The image monitoring of operating rooms improves practices in transfusion medicine; recent result. (2014)
  • Author(s): Furumaki H, Yamada C, Watanabe H, Fujihara H, Shibata H, Nagai S, Ishizuka K, Kaneko M, Daisuke S, Adachi M, Takeshita A.
  • Organizer: International Society of Blood Transfusion
  • Place of Presentation: Soul
  • Year and Date: 2014-06-01 – 2014-06-04
• [Presentation] CD56 expression is one of the unfavorable prognostic factors for acute promyelocytic leukemia patients: Long-term follow-up results of the Japan Adult Leukenia Study Group. (2013)
  • Author(s): Takeshita A, Ono T, Ohnishi K, Kishimoto Y, Kiyoi H, Okada M, Yamauchi T, Emi N, Fujita H, Horikawa K, Matsuda M, Shinagawa K, Monma F, Ohtake S, Nakaseko C, Takahashi M, Kimura Y, Iwanaga M, Asou N, Miyawaki S, Miyazaki Y, Naoe T.
  • Organizer: Promyelocytic Leukemia Symposium
  • Place of Presentation: Rome, Italy
• [Presentation] The efficacy of image monitoring of operating rooms in transfusion medicine. (2013)
  • Author(s): Furumaki H, Yamada C, Shibata H, Nagai S，Ishizuka K, Fujihara H, Watanabe H, Asahina A, Shimizu D, Takeshita A
  • Organizer: 18th Congress of European Hematology Association
  • Place of Presentation: Stockholm, Sweden
• [Presentation] Difference In Erythrocyte Alloantibodies After Blood Transfusion In Patients With Hematological and Non-Hematological Diseases. (2013)
  • Author(s): Shimizu D, Fujihara H, Shibata H, Yamada C, Furumaki H, Watanabe H, Ishizuka K, Ono T, Sakaguchi K, Ogata T, Takeshita A
  • Organizer: 55th Annual Meeting of the American Society of Hematology
  • Place of Presentation: New Orleans, USA
• [Presentation] Long-Term Outcome Of Acute Promyelocytic Leukemia (APL) With Lower Initial Leukocyte Counts By Using All-Trans Retinoic Acid (ATRA) Alone For Remission Induction Therapy: (2013)
  • Author(s): Ono T, Takeshita A, Kishimoto Y, Kiyoi H, Okada M, Yamauchi T, Emi N, Horikawa K, Matsuda M, Shinagawa K, Monma F, Ohtake S, Nakaseko C, Takahashi M, Kimura Y, Iwanaga M, Asou N, Naoe T;
  • Organizer: 55th Annual Meeting of the American Society of Hematology
  • Place of Presentation: New Orleans, USA
• [Book] calicheamicin conjugated monoclonal antibody therapy. Resistance to Immunotoxicins in Cancer Therapy (2015)
  • Author(s): Adachi M, Takeshita A
  • Total Pages: 156
  • Publisher: Springer