Development of the luciferase immunoprecipitation systems with high detection densitivity for anti-AQP4 antibody in neuromyelitis optica
| Project/Area Number |
24590720
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Nagasaki Kawatana Medical Center |
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Principal Investigator |
HIGUCHI osamu 独立行政法人国立病院機構長崎川棚医療センター(臨床研究部), その他部局等, 研究員 (50361720)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUO Hidenori 長崎川棚医療センター, 副院長 (20380975)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | NMO / 自己抗体 / AQP4 / ルシフェラーゼ / LIPS / 抗体検査 / 膜蛋白質 / 免疫沈降 / MS / 血清 |
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| Outline of Final Research Achievements |
Anti-AQP4 antibody in patient serum with neuromyelitis optica (NMO) is thought to be one of key molecules in the molecular pathogenesis of NMO. In addition, the quick inspection of the antibody concerned is an important matter in treatment strategy development of NMO, and the inspection system which is high-speed for high sensitivity is essential. Reliability of cell-based assay (CBA) which made full use of a fluorescent microscope system was the highest in the law conventionally, but problems about complexity and the quantifiability in the maneuver of the inspection had it pointed out. In this study, CBA problems include clear all, and, reliability was also succeeded in building a fully collateral has been innovative antibody test system.
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Report
(4 results)
Research Products
(8 results)
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[Presentation] Anti-potassium channel KIR4.1 antibody in multiple sclerosis and neuromyelitis optica2013
Author(s)
Shunya Nakane 1,2, Osamu Higuchi 1, Waka Sakai 2, Masaaki Niino 3,4, Toshiyuki Fukazawa 5, Seiji Kikuchi 4, Toshiyuki Takahashi 6, Kazuo Fujihara 6, Hidenori Matsuo
Organizer
6th Congress of the Pan-Asian Committee for treatment and research in Multiple Sclerosis
Place of Presentation
京都
Related Report
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