Visualization of neural circuits for pain processing using genetically engineered mice

• Project/Area Number: 24590736
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pain science
• Research Institution: Kansai Medical University
• Principal Investigator: KAZUHIKO Nishida 関西医科大学, 医学部, 助教 (80448026)
• Co-Investigator(Kenkyū-buntansha): ITO Seiji 関西医科大学, 医学部, 教授 (80201325) ; MATSUMURA Shinji 関西医科大学, 医学部, 講師 (70276393)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: カルシウムイメージング / 脊髄後角 / 体性感覚 / 脊髄 / 疼痛

Outline of Final Research Achievements

To gain insight of how cutaneous sensory stimulation is processed and trasmitted to the brain, the present study focused on the establishment of in vivo calcium imaging of a large ensemble of neurons in the spinal dorsal horn critical for transmission of sensory information. This study revealed that cutaneous pinch, brush, and heat stimulation, and stimulation to different skin points triggered neuronal activities of different subsets of neurons. This result suggests that each subset of spinal dorsal horn neurons is involved in neuronal processing of different types of sensory stimulation.

Research Products

• [Journal Article] In vivo two-photon imaging of structural dynamics in the spinal dorsal horn in an inflammatory pain model. (2015)
  • Author(s): Matsumura S, Taniguchi W, Nishida K, Nakatsuka T, Ito S
  • Journal Title: European Journal of Neuroscience Volume: 41 Issue: 7 Pages: 987-995
  • DOI: 10.1111/ejn.12837
  • Peer Reviewed / Open Access
• [Journal Article] Three-dimensional distribution of sensory stimulation-evoked neuronal activity of spinal dorsal horn neurons analyzed by in vivo calcium imaging (2014)
  • Author(s): Nishida K, Matsumura S, Taniguchi W, Uta D, Furue H, Ito S.
  • Journal Title: PLoS One Volume: 9(8) Issue: 8 Pages: e103321-e103321
  • DOI: 10.1371/journal.pone.0103321
  • Peer Reviewed / Open Access
• [Presentation] In vivo calcium imaging of spinal dorsal horn neurons in response to cutaneous sensory stimulation toward different skin points. (2014)
  • Author(s): 西田和彦、松村伸治、伊藤誠二
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-09-11
• [Presentation] 成熟した疼痛研究の新しい展開 (2014)
  • Author(s): 伊藤誠二、片野泰代、松村伸治、西田和彦
  • Organizer: 日本ペインクリニック学会第48回大会
  • Place of Presentation: 新宿京王プラザホテル
  • Year and Date: 2014-07-25
  • Invited
• [Presentation] In vivo カルシウムイメージングによる脊髄後角ニューロンの神経活動の三次元分布の解析 (2013)
  • Author(s): 西田和彦、松村伸治、伊藤誠二
  • Organizer: 第３６回日本神経科学大会
  • Place of Presentation: 国立京都国際会館
• [Book] 分子脳科学 (2015)
  • Author(s): 西田和彦、伊藤誠二 他 （三品昌美編）
  • Total Pages: 312
  • Publisher: 化学同人
• [Book] 分子脳科学(仮題） (2014)
  • Author(s): 西田和彦、伊藤誠二
  • Total Pages: 10
  • Publisher: 化学同人
• [Remarks] 関西医科大学医化学講座のホームページ
  • URL: http://www3.kmu.ac.jp/medchem