Environmental and genetic factors for the development of gallbladder cancer in Peruvians

Research Project

Project/Area Number	24590767
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Hygiene
Research Institution	Niigata University of Health and Welfare
Principal Investigator	Yamamoto Masaharu 新潟医療福祉大学, 健康科学部, 教授 (40018693)
Co-Investigator(Kenkyū-buntansha)	ENDOH Kazuo 新潟医療福祉大学, 健康栄養学科, 教授 (60176790) TSUCHIYA Yasuo 新潟大学, 医歯学総合研究科, 客員研究員 (60334679) ASAI Takao 新潟医療福祉大学, 臨床技術学科, 助教 (60612736)
Project Period (FY)	2012-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000) Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000) Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords	胆嚢がん / 環境要因 / 遺伝要因 / ペルー / 国際情報交換 / 遺伝子変異 / TP53 / K-ras / 赤唐辛子 / 発症要因 / オクラトキシン　A
Outline of Final Research Achievements	The concentrations of aflatoxin (AF) B1, B2, G1, G2 or ochratoxin A (OTA) in red chili peppers from Peru were measured using HPLC methods, and AFB1 and OTA were detected. The mean concentrations of OTA in red chili peppers from Chile, Bolivia, and Peru were higher in the order of the incidence of gallbladder cancer (GBC) among the three countries. These findings suggest the possibility that mycotoxin contamination of red chili peppers are associated with developing GBC. The frequency of TP53 and K-ras mutations in DNA extracted from paraffin embedded tissues of GBC patients were examined. TP53 mutations were found in 7 patients (7/18, 39%), namely 4 of transition type, 2 of transversion type, and 1 of insertion type. However, G:C to T:A transversion suggesting the presence of exogenous carcinogens, and K-ras mutation were not found. Our findings suggest that the development of GBC in Peruvians is more strongly associated with endogenous mechanisms rather than exogenous carcinogens.