Evaluation for neurodevelopmental deficit by nanomaterials
| Project/Area Number |
24590774
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | National Institute for Environmental Studies |
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Principal Investigator |
ISHIDO Masami 独立行政法人国立環境研究所, 環境リスク研究センター, 主任研究員 (60211728)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 銀ナノ粒子 / 神経発生毒性 / 神経系発達障害 / ナノ材料 |
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| Outline of Final Research Achievements |
We first analyzed the particle size of the AgNP used in this study by DLS . The maximum peak in DLS analysis was observed at 10 nm. We applied a neurosphere assay for neurodevelopmental effects of AgNPs. AgNPs inhibited cell migration in a dose-dependent manner. There was a linear correlation between the inhibition of migration and the logarithm of the particle concentration . Preceding migrated cells were retarded and/or collapsed by exposure to AgNPs: lower doses of AgNPs (0.31～1.25 μg/ml) caused a 42% retardation for 48 h, while higher doses of AgNPs (2.5～10 μg/ml) collasped migrating cells. Furthermore, collapsed cells were TUNEL-positive and showed a defect in the mitochondrial membrane potential.
We also examined the effects of AgNPs on rat behavior. A single oral administration of bisphenol A into 5-day-old male Wistar rats caused significant hyperactivity. It was about 1.3～1.4 fold more active in the nocturnal phase than control rats.
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Report
(4 results)
Research Products
(16 results)
