| Project/Area Number |
24590846
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Public health/Health science
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| Research Institution | Fukuoka Institute of Health and Environmental Sciences |
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Principal Investigator |
MURAKAMI Koichi 福岡県保健環境研究所, その他部局等, 研究員 (70446839)
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| Co-Investigator(Renkei-kenkyūsha) |
KAWAMURA Yoshiaki 愛知学院大学, 薬学部, 教授 (80262757)
TAKENAKA Shigeyuki 福岡県保健環境研究所, 計測技術課長 (60446838)
ETOH Yoshiki 福岡県保健環境研究所, 研究員 (50446845)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Escherichia albertii / Shiga toxine / Chicken meat / Infection disease / Foodborne disease / Pigeon / wild bird / 感染症 / 志賀毒素 / 人獣共通感染症 / 野鳥 / 食中毒 / 鶏肉 / eae / 大腸菌 / 志賀毒素2f / 該当せず |
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| Outline of Final Research Achievements |
We isolated or collected 106 Escherichia albertii isolates showing unique PFGE profiles. All of the isolates showed positive-indole/positive-lysine phenotypes. These characters are not identical to E. albertii biotype 1 or biotype 2. We also reported that the previously-identified Shiga toxin (Stx) 2f-producing Escherichia coli O115:HNM strain F08/101-31, from a symptomatic human, was confirmed in the current study to be Escherichia albertii by whole genome DNA-DNA hybridization, sequencing of the cpn60, dnaJ, and 16S rRNA genes, and by multi-locus sequence typing. Moreover, we reported the primers EA0134-283F/EA0134-446R may be needed for detection of E. albertii in food samples. We also reported E. albertii isolation from retail food, suggesting that chicken products are a potential vehicle of E. albertii transmission.
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