Mechanism of aversion to carbohydrate in citrin deficiency

• Project/Area Number: 24590892
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: Kagoshima University
• Principal Investigator: USHIKAI Miharu 鹿児島大学, 医歯(薬)学総合研究科, 助教 (70232816)
• Co-Investigator(Kenkyū-buntansha): TAKEYORI Saheki 熊本大学, 生命資源研究・支援センター, 特任教授 (10056070) ; AKIHIRO Asakawa 鹿児島大学, 医歯学総合研究科, 准教授 (10452947)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: シトリン欠損症 / 食行動解析 / 行動学 / 栄養学

Outline of Final Research Achievements

In this research, we analyzed the mechanism of aversion to carbohydrate in citrin deficiency using a model mouse. Our analysis on the appetite-related neuropeptides revealed a higher expression of urocortin1 (Ucn1) in the model mouse under the fed conditions.Oral sucrose administration significantly increased blood lactate concentration in the model mouse as compared with the wild-type, suggesting that the increases in blood lactate and Ucn1 expression in the central nervous system are related to the aversion in the model mouse.

Research Products

• [Journal Article] Mechanism for increased hepatic glycerol synthesis in the citrin/mitochondria glycerol-3-phosphate dehydrogenase double-knockout mouse: Urine glycerol and glycerol 3-phosphate as potential diagnostic markers of human citrin deficiency. (2015)
  • Author(s): Moriyama M, Fujimoto Y, Rikimaru S,Ushikai M, Kuroda E, Kawabe K, Takano K,Asakawa A, Inui A, Eto K, Kadowaki T, Sinasac DS, Okano Y, Yazaki M, Ikeda SI,Zhang C, Song YZ,Sakamoto O, Kure S, Mitsubuchi H, Endo F, Horiuchi M, Nakamura Y, Yamamura KI, Saheki T.
  • Journal Title: Biochim Biophys Acta. Volume: 印刷中 Issue: 9 Pages: 1787-1795
  • DOI: 10.1016/j.bbadis.2015.04.023
  • Peer Reviewed / Open Access
• [Journal Article] Rice koji reduced body weight gain, fat accumulation, and blood glucose level in high-fat diet-induced obese mice. (2014)
  • Author(s): Yoshizaki Y, Kawasaki C, Cheng KC, Ushikai M, Amitani H, Asakawa A, Okutsu K, Sameshima Y, Takamine K, Inui A.
  • Journal Title: PeerJ. Volume: 2 Pages: e540-e540
  • DOI: 10.7717/peerj.540
  • Peer Reviewed / Open Access
• [Journal Article] Voluntary exercise under a food restriction condition decreases blood branched-chain amino acid levels, in addition to improvement of glucose and lipid metabolism, in db mice, animal model of type 2 diabetes (2014)
  • Author(s): Marchianti AC, Arimura E, Ushikai M, Horiuchi M
  • Journal Title: Environ Health Prev Med Volume: 19 Issue: 5 Pages: 339-347
  • DOI: 10.1007/s12199-014-0400-z
  • NAID: 50009312840
  • Peer Reviewed / Open Access
• [Journal Article] Effects of aging on the plasma levels of nesfatin-1 and adiponectin. (2014)
  • Author(s): Li JB, Nishida M, Kaimoto K, Asakawa A, Chaolu H, Cheng KC, Li YX, Terashi M, Koyama KI, Amitani H, Sakoguchi T, Ushikai M, Ikeda S, Aoyama K, Horiuchi M, Li JZ, Inui A.
  • Journal Title: Biomed Rep. Volume: 2(1) Pages: 152-156
  • Peer Reviewed / Open Access
• [Journal Article] Effects of supplementation on food intake, body weight and hepatic metabolites in the citrin/mitochondrial glycerol-3-phosphate dehydrogenase double-knockout mouse model of human citrin deficiency (2012)
  • Author(s): Saheki T, Inoue K, Ono H, Katsura N, Yokogawa M, Yoshidumi Y, Furuie S, Kuroda E, Ushikai M, Asakawa A, Inui A, Eto K, Kadowaki T, Sinasac DS, Yamamura K, Kobayashi K
  • Journal Title: Mol Genet Metab Volume: 107(3) Issue: 3 Pages: 322-9
  • DOI: 10.1016/j.ymgme.2012.07.021
  • Peer Reviewed
• [Presentation] 糖尿病モデルマウスにおける運動負荷方法の違いがアミノ酸代謝に及ぼす影響 (2015)
  • Author(s): 牛飼美晴、Ancah Caesarina Novi Marchianti、Pulong Wijang Pralampita、Li Qi、 上野真忠、玉利千鶴、有村恵美、青山公治、堀内正久
  • Organizer: 第85回日本衛生学会学術総会
  • Place of Presentation: 和歌山県民文化会館(和歌山市)
  • Year and Date: 2015-03-28
• [Presentation] 摂食制限下の自発運動は、2型糖尿病モデルマウスの糖・脂質代謝とともにアミノ酸代謝も改善する (2015)
  • Author(s): 牛飼美晴、Ancah Caesarina Novi Marchianti、Pulong Wijang Pralampita、中野恵美子、有村恵美、堀内正久
  • Organizer: 第18回日本病態栄養学会年次学術集会
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2015-01-10
• [Presentation] 鹿児島大学医歯学総合研究科分子病態生化学において解析した尿素サイクル関連異常症例． (2012)
  • Author(s): 佐伯武頼、古家澄江、山村研一、牛飼美晴、浅川明弘、乾明夫、矢崎正英、池田修一、小林圭子
  • Organizer: 第54回日本先天代謝異常学会総会
  • Place of Presentation: 岐阜県岐阜市
• [Presentation] CTLN2発症に関わる遺伝要因の検索：ミトコンドリアglycerol -3phosphate dehydrogenase． (2012)
  • Author(s): 佐伯武頼、古家澄江、山村研一、井ノ上逸朗、牛飼美晴、浅川明弘、乾明夫、矢崎正英、池田修一、小林圭子
  • Organizer: 第54回日本先天代謝異常学会総会
  • Place of Presentation: 岐阜県岐阜市
• [Presentation] Increased hepatic glycerol synthesis in citrin deficiency model mouse.
  • Author(s): Moriyama M, Ushikai M, Kuroda E, Inui A, Takano K, Nakamura Y, Saheki T.
  • Organizer: 3rd ACIMD/55th JSIMD
  • Place of Presentation: 千葉県浦安市舞浜