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Development of membrane protein function analysis and the new suppression mediated by the tumor necrosis factor precursor and producing enzyme

Research Project

Project/Area Number 24590949
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Gastroenterology
Research InstitutionNagoya City University

Principal Investigator

JOH Takashi  名古屋市立大学, 医学(系)研究科(研究院), 教授 (30231369)

Co-Investigator(Kenkyū-buntansha) TANIDA Satoshi  名古屋市立大学, 大学院医学研究科, 講師 (30528782)
Co-Investigator(Renkei-kenkyūsha) HIGASHIYAMA Shigeki  愛媛大学, 大学院医学系研究科, 教授 (60202272)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
KeywordsADAM17 / ARP36-2 / TNFα / 新規治療ターゲット / 炎症性腸疾患 / TNF-α
Outline of Final Research Achievements

Colon epithelial cells, many expressed in monocyte cells I was paying attention to the ARP 36-2 seen. When IL-1β stimulates colon epithelial cell line, TNFα concentration in the culture solution in over time increased but TPA, LPS, increase was observed. On the other hand, when the monocytic cell line TPA stimulation, TNFα concentration in the culture solution is increased, IL-1β, the stimulation with TPA, increase was observed. Further, when the deletion of the ADAM17 by KB-R7785, siRNA, colon epithelial cells, IL-1β in the monocyte cell, the concentration increase due to TPA stimulation during TNFαshedding, was significantly inhibited. Furthermore, even if lacking the ARP36-2 by siRNA, similarly concentration rise due to IL-1β, TPA stimulus during TNFαshedding has been significantly suppressed. ARP36, it seemed to be made in inflammatory bowel disease novel therapeutic target to control the TNFαshedding.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (7 results)

All 2013 2012 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (2 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Annexin A2 regulates a disintegrin and metalloproteinase 17-mediated ectodomain shedding of pro-tumor necrosis factor-α in monocytes and colon epithelial cells.2013

    • Author(s)
      Tsukamoto H, Tanida S, Ozeki K, Ebi M, Mizoshita T, Shimura T, Mori Y, Kataoka H, Kamiya T, Fukuda S, Higashiyama S, Joh T.
    • Journal Title

      Inflamm Bowel Dis.

      Volume: 19 Issue: 7 Pages: 1365-1373

    • DOI

      10.1097/mib.0b013e318281f43a

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Annexin A2 regulates a disintegrin and metalloproteinase 17-mediated ectodomain shedding of pro-tumor necrosis factor-α in monocytes and colon epithelial cells.2013

    • Author(s)
      Hironobu Tsukamoto, Satoshi Tanida, Keiji Ozeki, Shigeki Higashiyama, and Takashi Joh
    • Journal Title

      Inflammatory Bowel Diseases

      Volume: in press

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 大腸上皮および単球細胞におけるamphiregulin-regulating protein(ARP) 36によるTNF-α放出制御機構2012

    • Author(s)
      塚本宏延、谷田諭史、尾関啓司、平田慶和、片岡洋望、城 卓志
    • Journal Title

      Ulcer Research

      Volume: 39 Pages: 192-195

    • NAID

      10031061741

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 大腸上皮および単球細胞におけるamphiregulin-regulating protein (ARP) 36によるTNFα放出制御機構

    • Author(s)
      塚本宏延,谷田諭史,尾関啓司,溝下勤,森義徳,片岡洋望,神谷武,城 卓志
    • Organizer
      JDDW
    • Place of Presentation
      神戸国際展示場(神戸市中央区)
    • Related Report
      2012 Research-status Report
  • [Presentation] 大腸上皮および単球細胞におけるamphiregulin-regulating protein (ARP) 36によるTNFα放出制御機構

    • Author(s)
      塚本宏延,谷田諭史,尾関啓司,溝下勤,城 卓志
    • Organizer
      第9回消化管の炎症を考える会
    • Place of Presentation
      トップオブスクエア(東京都千代田区)
    • Related Report
      2012 Research-status Report
  • [Remarks] 名古屋市立大学大学院医学研究科消化器・代謝内科学 研究業績

    • URL

      http://www.ncu-shotai.ac/index.html

    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 炎症性疾患治療薬のスクリーニング方法、並びに炎症性疾患の治療及び検査2012

    • Inventor(s)
      谷田諭史、城 卓志、東山繁樹、塚本宏延
    • Industrial Property Rights Holder
      谷田諭史、城 卓志、東山繁樹、塚本宏延
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-11-11
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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