The analysis of the function and the clinical application of REIC/Dkk-3 gene in digestive organ cancer

• Project/Area Number: 24590977
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Okayama University
• Principal Investigator: KATO Hironari 岡山大学, 大学病院, 助教 (60619039)
• Co-Investigator(Kenkyū-buntansha): TSUTSUMI Koichiro 岡山大学, 岡山大学病院, 助教 (40610910) ; SHIRAHA Hidenori 岡山大学, 岡山大学病院, 講師 (40379748) ; YAMAMOTO Kazuhide 岡山大学, 大学院医歯薬学総合研究科, 教授 (90140491) ; NASU Yasutomo 岡山大学, 岡山大学病院, 教授 (20237572)
• Research Collaborator: UCHIDA Daisuke 岡山大学, 岡山大学病院, 医員 (50749215)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: REIC/Dkk-3 / 膵臓癌 / 遺伝子治療 / 癌抑制遺伝子 / REIC遺伝子 / 消化器癌 / 膵癌 / 癌遺伝子治療

Outline of Final Research Achievements

REIC/Dkk-3, tumor suppressor gene, is down-regulated in various malignant tumors. In the current study, we assessed the effects of REIC/Dkk-3 gene therapy in pancreatic cancer. The REIC/Dkk-3 expression was lost in the pancreatic cancer cells, and tissues. Overexpression of REIC/Dkk-3 induced the apoptosis of the pancreatic cancer cells and inhibited tumor growth in xenograft mice model. REIC/Dkk-3 gene therapy is an attractive therapeutic tool for the pancreatic cancer.

Research Products

• [Journal Article] Potential of adenovirus-mediated REIC/Dkk-3 gene therapy for use in the treatment of pancreatic cancer. (2014)
  • Author(s): Uchida D, Shiraha H, Kato H, Nagahara T, Iwamuro M, Kataoka J, Horiguchi S, Watanabe M, Takaki A, Nouso K, Nasu Y, Yagi T, Kumon H, Yamamoto K.
  • Journal Title: J Gastroenterol Hepatol Volume: 29 Issue: 5 Pages: 973-983
  • DOI: 10.1111/jgh.12501
  • Peer Reviewed
• [Presentation] REIC/Dkk-3遺伝子による膵癌治療 (2014)
  • Author(s): 内田大輔、白羽英則、山本和秀
  • Organizer: JDDW2014
  • Place of Presentation: 東京
  • Year and Date: 2014-10-23
• [Presentation] The efficacy of REIC/Dkk-3 gene therapy for pancreatic cance (2014)
  • Author(s): 内田大輔、白羽英則、加藤博也
  • Organizer: DDW2014
  • Place of Presentation: シカゴ（アメリカ）
  • Year and Date: 2014-05-03
• [Presentation] The efficacy of REIC/Dkk-3 gene therapy for pancreatic cancer (2014)
  • Author(s): 内田大輔、白羽英則、加藤博也、永原照也、岩室雅也、片岡淳朗、堀口繁、渡部昌美、高木章乃夫、能祖一裕、那須保友、八木孝仁、公文裕巳、山本和秀
  • Organizer: Digestive Disease Week 2014
  • Place of Presentation: シカゴ（米国）
• [Presentation] REIC/Dkk-3遺伝子導入による膵癌治療の検討 (2013)
  • Author(s): 内田大輔、白羽英則、加藤博也、永原照也、片岡淳郎、岩室雅也、堀口繁、堤 康一郎、渡部昌実、那須保友、公文裕巳、山本和秀
  • Organizer: 第44回 日本膵臓学会大会
  • Place of Presentation: 仙台国際センター(仙台市)
• [Presentation] REIC/Dkk-3遺伝子導入による膵癌治療の検討 (2013)
  • Author(s): 内田大輔、白羽英則、加藤博也、永原照也、片岡淳郎、岩室雅也、堀口繁、堤 康一郎、渡部昌実、那須保友、公文裕巳、山本和秀
  • Organizer: 日本すい臓学会大会
  • Place of Presentation: 仙台国際センター