Analysis of Glomerular Injury: The Signal Transduction Pathway of Bowman's Epithelial Cells"

• Project/Area Number: 24591188
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Gunma University
• Principal Investigator: HIROMURA Keiju 群馬大学, 医学(系)研究科(研究院), 准教授 (70292597)
• Co-Investigator(Renkei-kenkyūsha): SAKAIRI Toru 群馬大学, 大学院医学系研究科, 助教 (20455976)
• Research Collaborator: STUART Shankland University of Washington, Division of Nephrology, Professor
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ボウマン嚢上皮細胞 / 糸球体障害 / sestrin 2 / mTOR経路 / ネフローゼ症候群 / 蛋白尿 / 国際情報交換 / 国際情報交換 米国

Outline of Final Research Achievements

In the current study, we have shown that sestrin 2, a novel stress-inducible protein, is selectively expressed Bowman's epithelial cells of normal rat kidney. In rat model of nephrotic syndrome (minimal change or focal segmental glomerulosclerosis) and crescentic glomerulonephritis, sestrin 2 expression was decreased, demonstrating that decreased expression of Sestrin 2 could be a novel marker of Bowman’s epithelial cell injury. In addition, mTOR pathway, which is the downstream of target of sestrin 2, was up- or down-regulated by the changes of sestrin 2 expression, suggesting that sestrin 2-mTOR pathway could be a target of treatment for glomerular injury.

Research Products

• [Journal Article] Expression of a novel stress-inducible protein, sestrin 2, in rat glomerular parietal epithelial cells (2014)
  • Author(s): Hiroko Hamatani, Keiju Hiromura, Toru Sakairi, Satoshi Takahashi, Mitsuharu Watanabe, Akito Maeshima, Takamoto Ohse, Jeffery W Pippin, Stuart J Shankland, Yoshihisa Nojima
  • Journal Title: American Journal of Physiology Renal Physiology Volume: 307 Issue: 6 Pages: F708-F717
  • DOI: 10.1152/ajprenal.00625.2013
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] ボーマン嚢上皮細胞における新規ストレス誘導蛋白sestrin-2の発現に関する検討 (2014)
  • Author(s): 浜谷博子、廣村桂樹、坂入 徹、渡辺光治、大瀬貴元, Shankland Stuart、野島美久
  • Organizer: 第5回分子腎臓フォーラム
  • Place of Presentation: 東京
  • Year and Date: 2014-09-06
• [Presentation] ボーマン嚢上皮細胞(PECs)における新規ストレス誘導蛋白Sestrin-2の検討
  • Author(s): 浜谷博子、廣村桂樹、坂入徹、高橋哲史、渡辺光治、前嶋明人、大瀬貴元, Shankland Stuart、野島美久
  • Organizer: 第56回日本腎臓学会学術総会
  • Place of Presentation: 東京国際フォーラム，東京
• [Presentation] EXPRESSION OF A NOVEL STRESS-INDUCIBLE PROTEIN, SESTRIN 2, IN RAT GLOMERULAR PARIETAL EPITHELIAL CELLS
  • Author(s): Hiroko Hamatani, Keiju Hiromura, Toru Sakairi, Satoshi Takahashi, Mitsuharu Watanabe, Akito Maeshima, Takamoto Ohse, Jeffery W. Pippin, Stuart J. Shankland, Yoshihisa Nojima
  • Organizer: The 14th Asian Pacific Congress of Nephrology 2014
  • Place of Presentation: Shinagawa Prince Hotel, Tokyo
• [Presentation] Expression of a novel stress-inducible protein, sestrin 2, in rat glomerular parietal epithelial
  • Author(s): Hiroko Hamatani, Keiju Hiromura, Toru Sakairi, Satoshi Takahashi, Mitsuharu Watanabe, Akito Maeshima, Takamoto Ohse, Jeffrey W Pippin, Stuart J Shankland, Yoshihisa Nojima
  • Organizer: 2012年米国腎臓学会
  • Place of Presentation: 米国・サンディエゴ
• [Presentation] ボーマン嚢上皮細胞(PECs)における新規ストレス誘導蛋白Sestrin-2の検討
  • Author(s): 浜谷博子、廣村桂樹、坂入徹、高橋哲史、渡辺光治、前嶋明人、大瀬貴元, Shankland Stuart、野島美久
  • Organizer: 第8回弥彦ポドサイトセミナー
  • Place of Presentation: コープシティ花園 （新潟県）