• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Role of a novel LST8-binding protein on mTOR signal and glucose and lipid metabolism

Research Project

Project/Area Number 24591315
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Metabolomics
Research InstitutionUniversity of Miyazaki (2014)
The University of Tokyo (2012-2013)

Principal Investigator

SAKODA Hideyuki  宮崎大学, 医学部, 講師 (50376464)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsインスリンシグナル伝達系 / mTOR
Outline of Final Research Achievements

mTOR signal is activated by overnutrition and obesity, and is strongly related with glucose and lipid metabolic disorder. The novel ProteinX was identified as a LST8, which is a common component of mTORC1 and mTORC2, by MEF-tagged pulldown assay. In this study I tried to reveal the role of ProteinX on mTOR signal. GST fusion protein of Protein X and adenovirus were constituted and the association between LST8 and ProteinX was examined by GST-pulldown assay and co-immunoprescipitation. But I could not prove the association between them. I speculate that ProteinX is the protein complex consisted with 8 isoforms, thus one of them can not bind with LST8.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results)

  • [Journal Article] Par14 associates with IRS-1, thereby enhancing insulin-induced IRS-1 phosphorylation and metabolic actions.2013

    • Author(s)
      Zhang J, Nakatsu Y, Sinjo T, Guo Y, Sakoda H, Yamamotoya T, Otani Y, Okubo H, Kushiyama A, Fujishiro M, Fukushima T, Tsuchiya Y, Kamata H, Nishimura F, Katagiri H, Takahashi SI, Kurihara H, Uchida T, Asano T.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 288 Issue: 28 Pages: 20692-20701

    • DOI

      10.1074/jbc.m113.485730

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Resistin-Like Molecule β Is Abundantly Expressed in Foam Cells and Is Involved in Atherosclerosis Development.2013

    • Author(s)
      Kushiyama A, Sakoda H, Oue N, Okubo M, Nakatsu Y, Ono H, Fukushima T, Kamata H, Nishimura F, Kikuchi T, Fujishiro M, Nishiyama K, Aburatani H, Kushiyama S, Iizuka M, Taki N, Encinas J, Sentani K, Ogonuki N, Ogura A, Kawazu S, Yasui W, Higashi Y, Kurihara H, Katagiri H, Asano T.
    • Journal Title

      Arteriosclerosis, Thrombosis, and Vascular Biology

      Volume: 33 Issue: 8 Pages: 1986-1993

    • DOI

      10.1161/atvbaha.113.301546

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lactobacillus casei strain Shirota protects against non-alcoholic steatohepatitis development in a rodent model2013

    • Author(s)
      Okubo H, Sakoda H, Kushiyama A, Fujishiro M, Nakatsu Y, Fukushima T, Matsunaga Y, Kamata H, Asahara T, Yoshida Y, Chonan O, Iwashita M, Nishimura F, Asano T
    • Journal Title

      American Journal Physiolgy-Gastrointestinal and Liver Physiogy

      Volume: 305 Issue: 12 Pages: 911-918

    • DOI

      10.1152/ajpgi.00225.2013

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Integrator complex plays anessential role inadipose differentiation2013

    • Author(s)
      Otani Y, Nakatsu Y, Sakoda H, Fukushima T, Fujishiro M, Kushiyama A, Okubo H, Tsuchiya Y, Ohno H, Takahashi S, Nishimura F, Kamata H, Katagiri H, Asano T.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 434 Issue: 2 Pages: 197-202

    • DOI

      10.1016/j.bbrc.2013.03.029

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Role of Pin1 in the pathogenesis of non-alcoholic steatohepatitis in a rodent model2012

    • Author(s)
      Nakatsu Y, Otani Y, Sakoda H, Zhang J, Guo Y, Okubo H, Kushiyama A, Fujishiro M, Kikuchi T, Fukushima T, Ohno H, Tsuchiya Y, Kamata H, Nagamachi A, Inaba T, Nishimura F, Katagiri H, Takahashi SI, Kurihara H, Uchida T, Asano T
    • Journal Title

      J Biol Chem

      Volume: 287 Issue: 53 Pages: 44526-44535

    • DOI

      10.1074/jbc.m112.397133

    • Related Report
      2012 Research-status Report
    • Peer Reviewed

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi