Role of a novel LST8-binding protein on mTOR signal and glucose and lipid metabolism

• Project/Area Number: 24591315
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: University of Miyazaki (2014); The University of Tokyo (2012-2013)
• Principal Investigator: SAKODA Hideyuki 宮崎大学, 医学部, 講師 (50376464)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: インスリンシグナル伝達系 / mTOR

Outline of Final Research Achievements

mTOR signal is activated by overnutrition and obesity, and is strongly related with glucose and lipid metabolic disorder. The novel ProteinX was identified as a LST8, which is a common component of mTORC1 and mTORC2, by MEF-tagged pulldown assay. In this study I tried to reveal the role of ProteinX on mTOR signal. GST fusion protein of Protein X and adenovirus were constituted and the association between LST8 and ProteinX was examined by GST-pulldown assay and co-immunoprescipitation. But I could not prove the association between them. I speculate that ProteinX is the protein complex consisted with 8 isoforms, thus one of them can not bind with LST8.

Research Products

• [Journal Article] Lactobacillus casei strain Shirota protects against nonalcoholic steatohepatitis development in a rodent model. (2015)
  • Author(s): Okubo H, Sakoda H, Kushiyama A, Fujishiro M, Nakatsu Y, Fukushima T, Matsunaga Y, Kamata H, Asahara T, Yoshida Y, Chonan O, Iwashita M, Nishimura F, Asano T
  • Journal Title: American Journal of Physiology –Gastrointestinal and Liver Physiology Volume: 305 Pages: 911-918
  • DOI: 10.1152/ajpgi.00225.2013
  • Peer Reviewed
• [Journal Article] Par14 protein associates with insulin receptor substrate 1 (IRS-1), thereby enhancing insulin-induced IRS-1 phosphorylation and metabolic actions. (2013)
  • Author(s): Zhang J, Nakatsu Y, Sinjo T, Guo Y, Sakoda H, Yamamotoya T, Otani Y, Okubo H, Kushiyama A, Fujishiro M, Fukushima T, Tsuchiya Y, Kamata H, Nishimura F, Katagiri H, Takahashi SI, Kurihara H, Uchida T, Asano T.
  • Journal Title: Journal of Biological Chemistry Volume: 288 Pages: 20692-20701
  • DOI: 10.1074/jbc.m113.485730
  • Peer Reviewed
• [Journal Article] Resistin-like molecule b in foam cells is involved in atherosclerosis development. (2013)
  • Author(s): Kushiyama A, Sakoda H, Oue N, Okubo M, Nakatsu Y, Ono H, Fukushima T, Kamata H, Nishimura F, Kikuchi T, Fujishiro M, Nishiyama K, Aburatani H, Kushiyama S, Iizuka M, Taki N, Encinas J, Sentani K, Ogonuki N, Ogura A, Kawazu S, Yasui W, Higashi Y, Kurihara H, Katagiri H, Asano T.
  • Journal Title: Arterioscler Thromb Vasc Biol. Volume: 33 Pages: 1986-1992
  • DOI: 10.1161/atvbaha.113.301546
  • Peer Reviewed / Open Access
• [Journal Article] Integrator complex plays an essential role in adipose differentiation. (2013)
  • Author(s): Otani Y, Nakatsu Y, Sakoda H, Fukushima T, Fujishiro M, Kushiyama A, Okubo H, Tsuchiya Y, Ohno H, Takahashi S, Nishimura F, Kamata H, Katagiri H, Asano T.
  • Journal Title: Biochem Biophys Res Commun Volume: 434 Pages: 0-0
  • DOI: 10.1016/j.bbrc.2013.03.029
  • Peer Reviewed
• [Journal Article] Role of Pin1 protein in the pathogenesis of nonalcoholic steatohepatitis in a rodent model (2012)
  • Author(s): Nakatsu Y, Otani Y, Sakoda H, Zhang J, Guo Y, Okubo H, Kushiyama A, Fujishiro M, Kikuchi T, Fukushima T, Ohno H, Tsuchiya Y, Kamata H, Nagamachi A, Inaba T, Nishimura F, Katagiri H, Takahashi SI, Kurihara H, Uchida T, Asano T
  • Journal Title: J Biol Chem Volume: 287 Pages: 44526-35
  • DOI: 10.1074/jbc.m112.397133
  • Peer Reviewed