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Elucidation of the mechanism of pancreatic beta-cell differentiation and regeneration using inducible cell labeling system

Research Project

Project/Area Number 24591330
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Metabolomics
Research InstitutionKobe University

Principal Investigator

MIANMI KOHTARO  神戸大学, 医学(系)研究科(研究院), 准教授 (80334176)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords糖尿病 / 糖代謝異常 / 膵β細胞 / 再生医療
Outline of Final Research Achievements

In the present study, we chased the fate of pancreatic beta cells by utilizing inducible cell labeling system. In alloxan-induced diabetic mice, the human GLP-1 receptor agonist liraglutide restored function and mass of beta cells. Liraglutide increased beta cell proliferation and decreased beta cell death, but did not induce neogenesis of beta cells.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (34 results)

All 2015 2014 2013 2012 Other

All Journal Article (12 results) (of which Peer Reviewed: 7 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (19 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Preferential gene expression and epigenetic memory of induced pluripotent stem cells derived from mouse pancreas2015

    • Author(s)
      Nukaya, D., Minami, K., Hoshikawa, R., Yokoi, N., Seino, S.
    • Journal Title

      Genes Cells

      Volume: 印刷中 Issue: 5 Pages: 367-381

    • DOI

      10.1111/gtc.12227

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Liraglutide improves pancreatic beta cell mass and function in alloxan-induced diabetic mice.2015

    • Author(s)
      Tamura K, Minami K, Kudo M, Iemoto K, Takahashi H, Seino S
    • Journal Title

      Plos One

      Volume: 10

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 膵再生2015

    • Author(s)
      南幸太郎
    • Journal Title

      肝胆膵

      Volume: 70 Pages: 441-446

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Glutamate acts as a key signal linking glucose metabolism to incretin/cAMP action to amplify insulin secretion2014

    • Author(s)
      Gheni G, Ogura M, Iwasaki M, Yokoi N, Minami K, Nakayama Y, Harada K, Hastoy B, Wu X, Takahashi H, Kimura K, Matsubara T, Hoshikawa R, Hatano N, Sugawara K, Shibasaki T, Inagaki N, Bamba T, Mizoguchi A, Fukusaki E, Rorsman P, Seino S
    • Journal Title

      Cell Rep

      Volume: 9 Issue: 2 Pages: 661-673

    • DOI

      10.1016/j.celrep.2014.09.030

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 膵腺房細胞から膵β細胞への分化転換2014

    • Author(s)
      南幸太郎
    • Journal Title

      胆と膵

      Volume: 35 Pages: 333-338

    • Related Report
      2014 Annual Research Report
  • [Journal Article] 膵臓の分化・再生研究の最前線2014

    • Author(s)
      南幸太郎
    • Journal Title

      日本消化器学会誌

      Volume: 111 Pages: 1543-1549

    • NAID

      130004678076

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Searching for stem cells in the adult pancreas: A futile effort?2013

    • Author(s)
      Minami K
    • Journal Title

      Journal of Diabetes Investigation

      Volume: 4 Issue: 4 Pages: 331-333

    • DOI

      10.1111/jdi.12061

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] GATA transcription factors: new key regulators in pancreas organogenesis.2013

    • Author(s)
      Minami K
    • Journal Title

      Journal of Diabetes Investigation

      Volume: 4 Issue: 5 Pages: 426-427

    • DOI

      10.1111/jdi.12089

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Conditional hypovascularization and hypoxia in islets does not overtly influence adult beta cell mass and function.2013

    • Author(s)
      D’Hoker J, De Leu N, Heremans Y, Baeyens L, Minami K, Ying C, Lavens A, Chintinne M, Stange G, Magenheim J, Swisa A, Martens G, Pipeleers D, van de Casteele M, Seino S, Keshet E, Dor Y, Himberg H
    • Journal Title

      Diabetes

      Volume: 62 Issue: 12 Pages: 4165-4173

    • DOI

      10.2337/db12-1827

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 膵β細胞の機能分化と再生誘導2013

    • Author(s)
      南 幸太郎
    • Journal Title

      腎と透析

      Volume: 75 Pages: 263-267

    • Related Report
      2013 Research-status Report
  • [Journal Article] Current status of regeneration of pancreatic -cells.2013

    • Author(s)
      Minami K, Seino S
    • Journal Title

      Journal of Diabetes Investigation

      Volume: 4 Issue: 2 Pages: 131-141

    • DOI

      10.1111/jdi.12062

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 膵β細胞におけるインスリン分泌制御メカニズム2012

    • Author(s)
      南幸太郎、清野進
    • Journal Title

      最新臨床糖尿病学(上)―糖尿病学の最新動向-

      Volume: NA Pages: 95-98

    • Related Report
      2012 Research-status Report
  • [Presentation] アロキサン糖尿病マウスにおけるリラグルチドの膵β細胞に対する効果2015

    • Author(s)
      田村香楠子、南幸太郎、高橋晴美 工藤麻耶、北野谷寿人、清野 進
    • Organizer
      第57回日本糖尿病学会年次学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2015-05-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] マウス膵外分泌細胞からのiPS細胞の樹立と特性解析2015

    • Author(s)
      南幸太郎、糠谷大樹、前田治代、星川律子、横井伯英、清野進
    • Organizer
      第57回日本糖尿病学会年次学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2015-05-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] Preferential expression of liver specific genes and epigenetic memory of induced pluripotent stem cells derived from mouse exocrine pancreas.2014

    • Author(s)
      Minami K, Nukaya D, Hoshikawa R, Yokoi N, Seino S
    • Organizer
      10th IDF-WPR Congress 2014/6th AASD Scientific Meeting
    • Place of Presentation
      Suntec, Singapore
    • Year and Date
      2014-11-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] 膵β細胞の3次元化によるインクレチン/cAMP応答性の誘導2014

    • Author(s)
      柴崎忠雄、吾甫尓江艾尼、マヒラアシム、岩崎真宏、星川律子、横井伯英、南幸太郎、清野進
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effect of liraglutide on beta-cell mass and function in alloxan diabetic mice.2014

    • Author(s)
      Tamura K, Minami K, Takahashi H, Kudo M, Seino S
    • Organizer
      50th Annual Meeting of the European Association for the Study of Diabetes
    • Place of Presentation
      Vienna, Austria
    • Year and Date
      2014-09-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 膵細胞の可塑性と再生医療への応用可能性2014

    • Author(s)
      南幸太郎
    • Organizer
      第4回細胞再生医療研究会
    • Place of Presentation
      神戸
    • Year and Date
      2014-07-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 膵β細胞におけるグルタミン酸動態の代謝フラックス解析2014

    • Author(s)
      吾甫尓江艾尼、横井伯英、星川律子、山口拓郎、岩崎真宏、小倉雅仁、南幸太郎、柴崎忠雄、清野進
    • Organizer
      第57回日本糖尿病学会年次学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2014-05-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Evaluation of the effect of liraglutide on beta cell fate in alloxan diabetic mice by tamoxifen-inducible Cre/loxP system.

    • Author(s)
      Tamura K, Minami K, Takahashi H, Kudo M, Kitanoya H, Seino S
    • Organizer
      49th Annual Meeting of the European Association for the Study of Diabetes
    • Place of Presentation
      Barcelona, Spain
    • Related Report
      2013 Research-status Report
  • [Presentation] The role of malate-aspartate shuttle-derived glutamate in incretin-potentiated insulin secretion.

    • Author(s)
      Ogura M, Iwasaki M, Gheni G, Minami K, Yokoi N, Shibasaki T, Seino S
    • Organizer
      第56回日本糖尿病学会年次学術集会
    • Place of Presentation
      熊本
    • Related Report
      2013 Research-status Report
  • [Presentation] Ins2-CreER/R26R-YFPダブルノックインマウスを用いた出生後における新生膵β細胞の運命追跡

    • Author(s)
      田村香楠子、南幸太郎、工藤麻耶、北野谷寿人、中村維文、家本啓佑、清野進
    • Organizer
      第56回日本糖尿病学会年次学術集会
    • Place of Presentation
      熊本
    • Related Report
      2013 Research-status Report
  • [Presentation] アロキサン誘導糖尿病マウスの膵細胞に対するリラグルチドの効果

    • Author(s)
      工藤麻耶、南幸太郎、田村香楠子、北野谷寿人、家本啓佑、高橋晴美、清野進
    • Organizer
      第56回日本糖尿病学会年次学術集会
    • Place of Presentation
      熊本
    • Related Report
      2013 Research-status Report
  • [Presentation] Induction of cAMP responsiveness by formation of pseudoislets.

    • Author(s)
      Gheni G, Iwasaki M, Ogura M, Yokoi N, Shibasaki T, Minami K, and Seino S
    • Organizer
      Beta Cell Workshop 2013 Kyoto
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] Effect of liraglutide on pancreatic beta-cell mass in alloxan induced diabetic mice.

    • Author(s)
      Kudo M, Minami K, Tamura K, Kitanoya H, Iemoto K, Takahashi H, and Seino S
    • Organizer
      Beta Cell Workshop 2013 Kyoto
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] Elucidation of postnatal pancreatic beta-cell fate using Ins2-CreER/R26R-YFP double knock-in mice.

    • Author(s)
      Tamura K, Minami K, Kudo M, Kitanoya H, Nakamura K, and Seino S
    • Organizer
      Beta Cell Workshop 2013 Kyoto
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] タモキシフェン誘導性細胞標識システムを用いた膵β細胞の運命追跡

    • Author(s)
      南幸太郎、清野進
    • Organizer
      第85回日本内分泌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
  • [Presentation] Progranulin (PGRN):インスリン抵抗性を惹起する新規アディポカイン

    • Author(s)
      松原稔哉、三田綾子、南幸太郎、細岡哲也、北澤荘平、高橋健一、田守義和、横井伯英、西村紀、清野進
    • Organizer
      第55回日本糖尿病学会年次学術集会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] Ins2-CreER/R26R-YFPダブルノックインマウスを用いた出生後における膵β細胞の特性分析

    • Author(s)
      田村香楠子、南幸太郎、中村維文、家本啓佑、清野進
    • Organizer
      第55回日本糖尿病学会年次学術集会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] 膵β細胞におけるインクレチン/cAMPシグナルとグルコース代謝の相互作用

    • Author(s)
      小倉雅仁、 岩崎真宏、吾甫尔江 艾尼、南幸太郎、柴崎忠雄、中山泰宗、原田和生、馬場健史、三木隆司、宮崎純一、福崎英一郎、清野進
    • Organizer
      第55回日本糖尿病学会年次学術集会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] 膵細胞間相互作用によるインクレチン/cAMPシグナル応答性の誘導

    • Author(s)
      吾甫尓江艾尼、岩崎真宏、小倉雅仁、柴崎忠雄、南幸太郎、清野進
    • Organizer
      第55回日本糖尿病学会年次学術集会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Book] 膵島の再生医療2015

    • Author(s)
      南幸太郎
    • Total Pages
      200
    • Publisher
      診断と治療社
    • Related Report
      2014 Annual Research Report
  • [Book] International Textbook of Diabetes Mellitus (ITDM), 4th edition2015

    • Author(s)
      Seino S, Shibasaki T, Minami K
    • Total Pages
      1240
    • Publisher
      Wiley-Blackwell
    • Related Report
      2014 Annual Research Report
  • [Remarks] 神戸大学細胞分子医学分野

    • URL

      http://www.med.kobe-u.ac.jp/phys1/

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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