| Project/Area Number |
24591345
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Osaka Medical College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SANO Hiroyuki 大阪医科大学, 医学部, 助教 (20556435)
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| Research Collaborator |
HASEDA Fumitaka 大阪医科大学, 医学部, ポストドクター
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 自己免疫性1型糖尿病 / 劇症1型糖尿病 / 制御性T細胞 / activated 制御性T細胞 / resting 制御性T細胞 / activated制御性T細胞 / Flow cytometry |
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| Outline of Final Research Achievements |
Regulatory T-cells (Tregs) play a central role in T-cell mediated immune response and the development of type 1 diabetes. CD4+FOXP3+ T-cells are composed of three phenotypically and functionally distinct subpopulations; r-Tregs, a-Tregs and non-Tregs. We aimed to clarify the frequency of these three subpopulations in CD4+FOXP3+ T-cells and the function of a-Tregs. We examined 20 patients with type 1A diabetes (T1AD),15 patients with fulminant type 1 diabetes (FT1D), 20 patients with type 2 diabetes (T2D) and 30 healthy control subjects (HC). The frequency of a-Tregs was significantly higher in T1AD, but not in FT1D, than the controls. Further, the proportion of a-Tregs among CD4+FOXP3+ T-cells was significantly higher in patients with T1AD with detectable C-peptide but not in patients with T1AD without it and with FT1D. A proliferation suppression assay showed that a-Tregs were functionally impaired both in FT1D and in T1AD.
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