| Project/Area Number |
24591416
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
KATSUMI Akira 浜松医科大学, 医学部, 准教授 (80378025)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUSHITA Tadashi 名古屋大学, 医学部附属病院, 教授 (30314008)
AMANO Mutsuki 名古屋大学, 大学院医学系研究科, 准教授 (90304170)
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| Co-Investigator(Renkei-kenkyūsha) |
MARUYAMA Mitsuo 国立長寿医療研究センター研究所, 老化機構研究部, 部長 (00212225)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | RhoF / filopodia / marzinal zone / knock out / Rhoファミリー / リンパ球 / ノックアウト / 分化 / 造血 / Rho ファミリー / フィロポディア |
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| Outline of Final Research Achievements |
RhoF is a member of the Rho GTPase family that has been implicated in various cell functions including long filopodia formation. Although RhoF is expressed in lymphoid tissues, the roles of RhoF in B cell development remain largely unclear. We analyzed B cell development in RhoF knockout (KO) mice and found a significant reduction in marginal zone (MZ) B cells in the spleen, although T cell development in the thymus and spleen was not affected. Consistent with these results, the width of the MZ B cell region in the spleen was significantly reduced in the RhoF KO mice. However, the antigen-specific antibody titer of IgM and IgG3 after MZ B cell-specific antigen (T cell-independent antigen, type I) stimulation was not affected by RhoF deletion. Furthermore, we demonstrated that RhoF was dispensable for stromal cell-derived factor-1α- and B lymphocyte chemoattractant-induced B cell migration. These results suggest that RhoF promotes MZ B cell development in the spleen.
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