RhoF promotes murine marginal zone B cell development
Project/Area Number |
24591416
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Hematology
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Research Institution | Hamamatsu University School of Medicine |
Principal Investigator |
KATSUMI Akira 浜松医科大学, 医学部, 准教授 (80378025)
|
Co-Investigator(Kenkyū-buntansha) |
MATSUSHITA Tadashi 名古屋大学, 医学部附属病院, 教授 (30314008)
AMANO Mutsuki 名古屋大学, 大学院医学系研究科, 准教授 (90304170)
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Co-Investigator(Renkei-kenkyūsha) |
MARUYAMA Mitsuo 国立長寿医療研究センター研究所, 老化機構研究部, 部長 (00212225)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | RhoF / filopodia / marzinal zone / knock out / Rhoファミリー / リンパ球 / ノックアウト / 分化 / 造血 / Rho ファミリー / フィロポディア |
Outline of Final Research Achievements |
RhoF is a member of the Rho GTPase family that has been implicated in various cell functions including long filopodia formation. Although RhoF is expressed in lymphoid tissues, the roles of RhoF in B cell development remain largely unclear. We analyzed B cell development in RhoF knockout (KO) mice and found a significant reduction in marginal zone (MZ) B cells in the spleen, although T cell development in the thymus and spleen was not affected. Consistent with these results, the width of the MZ B cell region in the spleen was significantly reduced in the RhoF KO mice. However, the antigen-specific antibody titer of IgM and IgG3 after MZ B cell-specific antigen (T cell-independent antigen, type I) stimulation was not affected by RhoF deletion. Furthermore, we demonstrated that RhoF was dispensable for stromal cell-derived factor-1α- and B lymphocyte chemoattractant-induced B cell migration. These results suggest that RhoF promotes MZ B cell development in the spleen.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] RhoF promotes murine marginal zone B cell development.2014
Author(s)
Kishimoto M, Matsuda T, Yanase S, Katsumi A, Suzuki N, Ikejiri M, Takagi A, Ikawa M, Kojima T, Kunishima S, Kiyoi H, Naoe T, Matsushita T, Maruyama M.
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Journal Title
Nagoya J Med Sci.
Volume: 76
Pages: 293-305
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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