Development of a novel antileukemia adoptive immunotherapy using leukemia-reactive gene-modified memory T cells.

• Project/Area Number: 24591426
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Ehime University
• Principal Investigator: FUJIWARA HIROSHI 愛媛大学, 医学部附属病院, 講師 (20398291)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 遺伝子細胞免疫療法 / T細胞受容体（TCR）遺伝子 / 遺伝子改変Tリンパ球 / 免疫記憶細胞 / 白血病幹細胞 / 細胞傷害性Tリンパ球（CTL） / T細胞受容体遺伝子（TCR）遺伝子 / 抗白血病効果 / 白血病性幹細胞 / 細胞免疫療法

Outline of Final Research Achievements

The clinical efficacy mediated by gene-modified CD8+ T-lymphocytes using leukemia antigen-specific T-cell receptor (TCR) gene transfer (artificial cytotoxic T lymphocytes: artificial CTL) depends on the in vivo persistence of those artificial CTL after recognition of leukemia cells. In this study, to this end, we assessed the helper function mediated by newly gene-modified CD4+ T-lymphocytes (artificial helper T cells) using the identical HLA class I-restricted TCR gene which was employed for artificial CTLs. In results, those artificial helper T cells successfully stimulated artificial CTLs to survive longer after recognition of leukemia cells in vivo through the facilitation of memory T-cell formation mediated by artificial CTL.

Research Products

• [Journal Article] Antileukemia multifunctionality of CD4+ T cells genetically engineered by HLA class I-restricted and WT1-specific T-cell receptor gene transfer. (2015)
  • Author(s): Fujiwara H*, Ochi T, Ochi F, Miyazaki Y, Asai H, Narita M, Okamoto S, Mineno J, Kuzushima K, Shiku H, Yasukawa M.
  • Journal Title: Lｅｕｋｅｍｉａ Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Optimization of T-cell reactivity y exploiting TCR chain centricity for the purpose of safe and effective antitumor TCR gene therapy. (2015)
  • Author(s): Ochi T, Nakatsugawa M, Chamoto K, Tanaka S, Yamashita Y, Guo T, Fujiwara H*, Yasukawa M, Butler M, Hirano N.
  • Journal Title: Cancer Immunology Research Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Dose-intensified chemotherapy alone or in combination with mogamulizumab in newly diagnosed aggressive adult T-cell leukaemia-lymphoma: a randomized phase II study. (2015)
  • Author(s): Ishida T, Jo T, Takemoto S, Suzushima H, Uozumi K, Yamamoto K, Uike N, Saburi Y, Nosaka K, Utsunomiya A, Tobinai K, Fujiwara H, Ishitsuka K, Yoshida S, Taira N, Moriuchi Y, Imada K, Miyamoto T, Akinaga S, Tomonaga M, Ueda R
  • Journal Title: Br J Haematol Volume: 未定 Issue: 5 Pages: 672-682
  • DOI: 10.1111/bjh.13338
  • Peer Reviewed
• [Journal Article] Cytotoxic T lymphocyte lysis of HTLV-1 infected cells is limited by weak HBZ protein expression, but non-specifically enhanced on induction of Tax expression. (2014)
  • Author(s): Rowan AG, Suemori K, Fujiwara H*, Yasukawa M, Tanaka Y, Taylor GP, Bangham C.
  • Journal Title: Retrovirology Volume: 11(1) Pages: 116-116
  • Peer Reviewed
• [Journal Article] Adoptive immunotherapy for hematological malignancies using T cells gene-modified to express tumor antigen-specific receptors. (2014)
  • Author(s): Fujiwara H
  • Journal Title: Pharmaceuticals Volume: 7(12) Issue: 12 Pages: 1049-1068
  • DOI: 10.3390/ph7121049
  • Peer Reviewed
• [Journal Article] 遺伝子改変T細胞による血液腫瘍に対する養子免疫療法 (2014)
  • Author(s): 藤原 弘
  • Journal Title: 臨床血液 Volume: 55(10) Pages: 2162-2174
  • NAID: 130004696111
• [Journal Article] 腫瘍抗原特異的T細胞受容体遺伝子導入を用いた遺伝子改変T細胞による抗白血病養子免疫療法 (2014)
  • Author(s): 藤原 弘
  • Journal Title: 臨床血液 Volume: 55(6) Pages: 657-669
  • NAID: 130004501866
• [Journal Article] TCR/CAR遺伝子導入T細胞を用いた養子免疫療法 (2014)
  • Author(s): 藤原 弘
  • Journal Title: 細胞 Volume: 46(2) Pages: 409-413
• [Journal Article] TCR/CAR遺伝子導入T細胞を用いた白血病・リンパ腫に対する養子免疫療法 (2014)
  • Author(s): 藤原 弘
  • Journal Title: 血液フロンティア Volume: 24(11) Pages: 45-53
• [Journal Article] Gene-Modified Human α/β-T Cells Expressing a Chimeric CD16-CD3ζ Receptor as Adoptively Transferable Effector Cells for Anticancer Monoclonal Antibody Therapy. (2014)
  • Author(s): Yasukawa M, et al.
  • Journal Title: Cancer Immunol Res Volume: 2 Issue: 3 Pages: 249-262
  • DOI: 10.1158/2326-6066.cir-13-0099-t
  • Peer Reviewed / Open Access
• [Journal Article] Adoptive transfer of genetically engineered WT1-specific cytotoxic T lymphocytes does not induce renal injury (2014)
  • Author(s): Asai H, Fujiwara H, Kitazawa S, Kobayashi N, Ochi T, Miyazaki Y, Ochi F, Akatsuka Y, Okamoto S, Mineno J, Kuzushima K, Ikeda H, Shiku H, Yasukawa M
  • Journal Title: J Hematol Oncol. Volume: 7 Issue: 1 Pages: 3-3
  • DOI: 10.1186/1756-8722-7-3
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genetically engineered fixed K562 cells: potent “off the shelf” antigen presenting cells for generating virus specific T cells. (2014)
  • Author(s): Tanimoto K, Muranski P, Miner S, Fujiwara H*, Kajigaya S, Keyvanfer K, Hensel N, Barrett AJ, Melenhorst JJ.
  • Journal Title: Cytotherapy Volume: 16(1) Issue: 1 Pages: 135-146
  • DOI: 10.1016/j.jcyt.2013.08.008
  • Peer Reviewed
• [Journal Article] Adoptive T-cell therapy for hematological malignancies using T cells gene-modified to express tumor antigen-specific receptors. (2014)
  • Author(s): Fujiwara H
  • Journal Title: International Journal of Hematology Volume: 99 Issue: 2 Pages: 123-131
  • DOI: 10.1007/s12185-013-1493-7
  • Peer Reviewed
• [Journal Article] Cutaneous lesions of blastic plasmacytoid dendritic cell neoplasm. (2014)
  • Author(s): Suemori K, Fujiwara H*, Niiya T, Yasukawa M.
  • Journal Title: Internal Medicine Volume: 53(4) Pages: 339-340
  • NAID: 130003392659
  • Peer Reviewed
• [Journal Article] Development of a novel redirected T-cell-based adoptive immunotherapy targeting human telomerase reverse transcriptase for adult T-cell leukemia. (2013)
  • Author(s): Yasukawa M, et al.
  • Journal Title: Blood Volume: 121 Issue: 24 Pages: 4894-4901
  • DOI: 10.1182/blood-2012-11-465971
  • Peer Reviewed
• [Journal Article] Adoptive transfer of genetically modified Wilms' tumor 1-specific T cells in a novel malignant skull base meningioma model. (2013)
  • Author(s): Iwami K, Natsume A, Ohno M, Ikeda H, Mineno J, Nukaya I, Okamoto S, Fujiwara H, Yasukawa M, Shiku H, Wakabayashi T.
  • Journal Title: Neuro Oncol. Volume: 15 Issue: 6 Pages: 747-758
  • DOI: 10.1093/neuonc/not007
  • Peer Reviewed
• [Journal Article] Co-introduced functional CCR2 potentiates in vivo anti-lung cancer func tionality mediated by T cells double gene-modified to express WT1-specific T-cell receptor. (2013)
  • Author(s): Yasukawa M, et al.
  • Journal Title: PLoS One Volume: 10 Issue: 2 Pages: e56820-e56820
  • DOI: 10.1371/journal.pone.0056820
  • Peer Reviewed
• [Journal Article] A promising vector for TCR gene therapy: differential effect of siRNA, 2A peptide, and disulfide bond on the introduced TCR expression. (2012)
  • Author(s): Okamoto S, et al.
  • Journal Title: Mol Ther Nucleic Acids. Volume: 18 Pages: e63-e63
  • DOI: 10.1038/mtna.2012.52
  • Peer Reviewed
• [Journal Article] HLA-restricted presentation of WT1 tumor antigen in B-lymphoblastoid cell lines established using a maxi-EBV system. (2012)
  • Author(s): Kanda, T, et al.
  • Journal Title: Cancer Gene Ther. Volume: 19 Issue: 8 Pages: 566-571
  • DOI: 10.1038/cgt.2012.34
  • Peer Reviewed
• [Journal Article] Defucosylated anti-CCR4 monoclonal antibody (KW-0761) for relapsed adult T-cell leukemia-lymphoma a multicenter phase II study (2012)
  • Author(s): Ishida T.
  • Journal Title: J Clin Oncol Volume: 30(8) Issue: 8 Pages: 837-42
  • DOI: 10.1200/jco.2011.37.3472
  • Peer Reviewed
• [Journal Article] Aurora kinase A-specific T-cell receptor gene transfer redirects T-lymphocytes to display effective anti-leukemia reactivity (2012)
  • Author(s): Kozo Nagai, et al
  • Journal Title: Blood Volume: 119(2) Issue: 2 Pages: 368-76
  • DOI: 10.1182/blood-2011-06-360354
  • Peer Reviewed
• [Presentation] 移植と抗腫瘍免疫療法 (2015)
  • Author(s): 藤原 弘*
  • Organizer: 第37回 日本造血細胞移植学会総会 教育講演3
  • Place of Presentation: 神戸市
  • Year and Date: 2015-03-05 – 2015-03-07
  • Invited
• [Presentation] Concomitant administration of gene-modified T cells expressing a chimeric CD16-CD3zeta receptor with Mogamulizmab synergistically suppresses adult T cell leukemia cells in vivo. (2014)
  • Author(s): Tanimoto K, Fujiwara H*, Tanaka H, Ochi F, Asai H, Okamoto S, Mineno J, Kuzushima K, Shiku H, Barrett AJ, Yasukawa M.
  • Organizer: 56th American Society of Hematology, Annual meeting and Exposition
  • Place of Presentation: San Francisco, CA
  • Year and Date: 2014-12-06 – 2014-12-09
• [Presentation] Activation Status of Integrin αIIbβ3 in Essential Thrombocythemia with Calreticulin Mutation. (2014)
  • Author(s): Yamanouchi J, Hato T, Matsubara E, Azuma T, Nakanishi H, Fujiwara H*, Yakushijin Y, Yasukawa M.
  • Organizer: 56th American Society of Hematology, Annual meeting and Exposition
  • Place of Presentation: San Francisco, CA
  • Year and Date: 2014-12-06 – 2014-12-09
• [Presentation] 遺伝子改変T細胞による血液腫瘍に対する養子免疫療法 (2014)
  • Author(s): 藤原 弘*
  • Organizer: 第76回日本血液学会学術集会 教育講演EL-56
  • Place of Presentation: 大阪市
  • Year and Date: 2014-10-31 – 2014-11-02
  • Invited
• [Presentation] Targeting Aurora Kinase A with an enhanced T-cell receptor gene-transfer vector. (2014)
  • Author(s): Hiroshi Fujiwara*, Nicholas Casey, Sachiko Okamoto, Junichi Mineno, Kiyotaka Kuzushima, Shiku Hiroshi, Masaki Yasukawa
  • Organizer: 第76回 日本血液学会学術集会 （English Session）
  • Place of Presentation: 大阪市
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] Aurora Kinase-A vaccination therapy in leukemia patients. (2014)
  • Author(s): 谷本一史、藤原 弘*、宮崎幸大、越智俊元、朝井洋晶、山之内 純、東 大地、羽藤高明、安川正貴
  • Organizer: 第76回 日本血液学会学術集会
  • Place of Presentation: 大阪市
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] Development of HLA class I-restricted Th1 and Th17 CD4+ T cells gene-modified to target WT1. (2014)
  • Author(s): 朝井洋晶、藤原 弘*、越智俊元、岡本幸子、峰野純一、葛島清隆、珠玖 洋、安川正貴
  • Organizer: 第76回 日本血液学会学術集会
  • Place of Presentation: 大阪市
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] ポテリジオを併用したATLに対する新たな細胞免疫療法の可能性. (2014)
  • Author(s): 田中寛希、藤原 弘*、越智史博、谷本一史、朝井洋晶、那波明宏、安川正貴
  • Organizer: 第76回 日本血液学会学術集会
  • Place of Presentation: 大阪市
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] High-quality TCR-gene modified T cells using siTCR lentiviral vector in combination with anti-CD3/CH296 stimulation. (2014)
  • Author(s): 岡本幸子、池田裕明、藤原 弘*、安川正貴、珠玖 洋、峰野純一
  • Organizer: 第73回日本癌学会学術集会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] モガムリツマブと人工CD16陽性T細胞を併用した成人T細胞白血病に 対する新規治療法開発. (2014)
  • Author(s): 田中寛希、藤原 弘*、谷本一史、越智史博、安川正貴
  • Organizer: 第73回日本癌学会学術集会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Generation of HLA class I-restricted TCR-gene modified CD4+ T-cell subtype for antileukemia adoptive immunotherapy. (2014)
  • Author(s): 朝井洋晶、藤原 弘*、越智史博、越智俊元、岡本幸子、峰野純一、葛島清隆、珠玖 洋、安川正貴
  • Organizer: 第73回日本癌学会学術集会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Myeloid leukemia cells suppress T cell proliferation. (2014)
  • Author(s): 谷本一史、藤原 弘*、安川正貴
  • Organizer: 第73回日本癌学会 学術集会 （English oral session）
  • Place of Presentation: 横浜市
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] TCR遺伝子導入T細胞を用いる養子免疫療法・最近の知見 (2014)
  • Author(s): 藤原 弘*
  • Organizer: 第6回 血液疾患免疫療法研究会 シンポジウム
  • Place of Presentation: 京都市
  • Year and Date: 2014-09-06
  • Invited
• [Presentation] High-quality TCR-gene modified T cells using siTCR lentiviral vector in combination with anti-CD3/CH296 stimulation. (2014)
  • Author(s): 岡本幸子、礒江加奈子、池田裕明、藤原 弘*、安川正貴、珠玖 洋、峰野純一
  • Organizer: 第20回日本遺伝子治療学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-08-06 – 2014-08-08
• [Presentation] がんに対するT細胞受容体（TCR）遺伝子導入T細胞を用いる細胞免疫療法 (2014)
  • Author(s): 藤原 弘*
  • Organizer: 第18回 日本がん免疫学会総会、シンポジウム2
  • Place of Presentation: 松山市
  • Year and Date: 2014-07-30 – 2014-08-01
  • Invited
• [Presentation] Myeloid leukemia cells suppress T cell proliferation. (2014)
  • Author(s): 谷本一史、Miner Samantha、伊藤沙和、Pawel Muranski、藤原 弘*、Nancy Hensel、J.Joseph Melenhorst、A John Barrett、安川正貴
  • Organizer: 第18回日本がん免 疫学会総会
  • Place of Presentation: 松山市
  • Year and Date: 2014-07-30 – 2014-08-01
• [Presentation] HLA class I拘束性WT1特異的Th1及びTh17 CD4+ T細胞作製 の試み. (2014)
  • Author(s): 朝井洋晶、藤原 弘*、越智俊元、岡本幸子、峰野純一、葛島清隆、珠玖 洋、安川正貴
  • Organizer: 第18回日本がん免 疫学会総会
  • Place of Presentation: 松山市
  • Year and Date: 2014-07-30 – 2014-08-01
• [Presentation] Targeting Aurora Kinase A with an enhanced TCR gene-transfer vector. (2014)
  • Author(s): Nicholas Casey、藤原 弘*、岡本幸子、峰野純一、葛島清隆、珠玖 洋、安川正貴
  • Organizer: 第18回日本がん免 疫学会総会
  • Place of Presentation: 松山市
  • Year and Date: 2014-07-30 – 2014-08-01
• [Presentation] ポテリジオを併用したATLに対する新たな細胞免疫療法の可能性. (2014)
  • Author(s): 田中寛希、藤原 弘*、越智史博、谷本一史、朝井洋晶、那波明宏、安川正貴
  • Organizer: 第18回日本がん免 疫学会総会
  • Place of Presentation: 松山市
  • Year and Date: 2014-07-30 – 2014-08-01
• [Presentation] Generation of high-quality TCR-gene modified T cells using siTCR lentiviral vector combined with anti-CD3/CH296 stimulation and transduction method. (2014)
  • Author(s): Okamoto S, Isoe K, Amaishi Y, Shimomura M, Ikeda H, Fujiwara H*, Yasukawa M, Shiku H, Mineno J.
  • Organizer: American Society of Gene & Cell Therapy, 17th Annual Meeting
  • Place of Presentation: Washington DC
  • Year and Date: 2014-05-21 – 2014-05-24
• [Presentation] Human telomerase reverse transcriptase-specific T-cell receptor gene transfer redirects T cells to display an effective antitumour reactivity against adult T-cell leukemia. (2013)
  • Author(s): Fujiwara H et al.
  • Organizer: 39th Annual Meeting of the European Group for Blood and Marrow Transplantation
  • Place of Presentation: London, UK
• [Presentation] 白血病治癒を目指す細胞免疫療法を開発する上で同種免疫反応は必要か？ (2013)
  • Author(s): 朝井洋晶 他.
  • Organizer: 第35回日本造血細胞移植学会
  • Place of Presentation: 石川県 金沢市
• [Presentation] 細胞療法への応用を目的としたADCC活性を発揮するエフェクターT細胞の開発。 (2013)
  • Author(s): 越智史博 他.
  • Organizer: 第35回日本造血細胞移植学会
  • Place of Presentation: 石川県 金沢市
• [Presentation] TCR遺伝子改変T細胞を用いて白血病性幹細胞を狙い撃つ。 (2013)
  • Author(s): 藤原 弘
  • Organizer: 第11回神奈川ヘマトロジーカンファレンス
  • Place of Presentation: 神奈川県 横浜市
  • Invited
• [Presentation] Human Telomerase Reverse Transcriptase-specific T-Cell Receptor Gene Transfer Redirects T Cells to Display an Effective Antitumor Reactivity Against Adult T-Cell Leukemia. (2012)
  • Author(s): Fujiwara H et al.
  • Organizer: The 54th Annual Meeting of the American Society of Hematology.
  • Place of Presentation: Atlanta, GA
• [Presentation] Redirected CD4+ T Cells towards HLA Class I-Restricted WT1 Epitope Successfully Display Multifactorial Helper Function for the Enhancement of Antileukemia Efficacy Mediated by Redirected T-Cell Based Immunocelltherapy. (2012)
  • Author(s): Miyazaki Y et al.
  • Organizer: The 54th Annual Meeting of the American Society of Hematology.
  • Place of Presentation: Atlanta, GA
• [Presentation] Therapeutically Infused Redirected T Cells Targeting WT1successfully Inhibited Leukemia Stem Cells in Vivo. (2012)
  • Author(s): Asai H et al.
  • Organizer: The 54th Annual Meeting of the American Society of Hematology.
  • Place of Presentation: Atlanta, GA
• [Presentation] Abrogation of Myeloid Derived Cell-like Inhibitory Activity of K562 cells Restores Antigen Presenting Cell Functions. (2012)
  • Author(s): Tanimoto K et al.
  • Organizer: The 54th Annual Meeting of the American Society of Hematology.
  • Place of Presentation: Atlanta, GA
• [Presentation] Active Application of Genetically Redirected CD4+ T cells Using Tumor Antigen-specific TCR Gene Transfer in Redirected T cell-based Antileukemia Adoptive Immunotherapy. (2012)
  • Author(s): Fujiwara H et al.
  • Organizer: The 3rd Workshop of Synthetic Immunology.
  • Place of Presentation: Kyoto, Japan
• [Presentation] Detailed analysis of helper functions mediated by genetically engineered CD4+ T cells to express HLA-A24 and WT1-specific T-cell receptor in redirected T cell-based adoptive immunotherapy against human leukemia. (2012)
  • Author(s): Fujiwara H et al.
  • Organizer: The 6th International Conference on WT1 in Human Neoplasia.
  • Place of Presentation: Kyoto, Japan
• [Presentation] HLA class I拘束性腫瘍抗原特異的TCRを遺伝子導入したCD4陽性T細胞の機能解析。 (2012)
  • Author(s): 宮崎幸大 他.
  • Organizer: 第16回日本がん免疫学会総会
  • Place of Presentation: 北海道 札幌市
• [Presentation] 経静脈的に輸注されたWT1特異的TCR 遺伝子導入Tリンパ球は骨髄へ移行し静止期の白血病性幹細胞を傷害出来る可能性がある。 (2012)
  • Author(s): 朝井洋晶 他.
  • Organizer: 第16回日本がん免疫学会総会
  • Place of Presentation: 北海道 札幌市
• [Presentation] 細胞免疫療法としての抗体依存性細胞傷害活性（ADCC）を発揮する新たな遺伝子改変Tリンパ球の作成。 (2012)
  • Author(s): 越智博史 他.
  • Organizer: 第16回日本がん免疫学会総会
  • Place of Presentation: 北海道 札幌市
• [Presentation] WT1を治療標的抗原とする白血病幹細胞制御を目指した細胞免疫療法の可能性の検討。 (2012)
  • Author(s): 藤原 弘 他.
  • Organizer: 第4回造血器腫瘍免疫療法研究会学術集会
  • Place of Presentation: 石川県 金沢市
• [Presentation] 成人T細胞白血病に対するhTERT特異的TCR遺伝子を用いた新たな細胞免疫療法の開発。 (2012)
  • Author(s): 宮崎幸大 他.
  • Organizer: 第4回造血器腫瘍免疫療法研究会学術集会
  • Place of Presentation: 石川県 金沢市
• [Presentation] Active application of leukemia antigen-specific T-cell receptor introduced CD4+ T cells immunocell therapy. (2012)
  • Author(s): Fujiwara H et al.
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 北海道 札幌市
• [Presentation] Next generation ”siTCR” retroviral vector to improve the efficacy and safety in TCR gene therapy. (2012)
  • Author(s): Okamoto S et al.
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 北海道 札幌市
• [Presentation] Redirected T-cell based immunotherapy targeting hTERT displays an anti-tumor reactivity against adult T cell leukemia. (2012)
  • Author(s): Ochi F et al.
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 北海道 札幌市
• [Presentation] Development of redirected T cell-based adoptive immunotherapy targeting leukemia stem cells. (2012)
  • Author(s): Asai H et al.
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 北海道 札幌市
• [Presentation] Development of redirected T cell-based adoptive immunotherapy targeting leukemia stem cells. (2012)
  • Author(s): Asai H et al.
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 京都市
• [Presentation] hTERT-specific TCR gene transduced T cells display an effective anti-tumor reactivity against ATL. (2012)
  • Author(s): Miyazaki Y et al.
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 京都市
• [Presentation] Multifactorial help by WT1-specific TCR gene transduced CD4+ T cells in T cell-based immunotherapy. (2012)
  • Author(s): Fujiwara H et al.
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 京都市
• [Presentation] Aurora kinase A-specific T-cell receptor gene transfer redirects T lymphocytes to display effective antileukemia reactivity. (2012)
  • Author(s): Fujiwara H
  • Organizer: The 10th Advanced Hematology Seminar.
  • Place of Presentation: Tokyo, Japan
  • Invited
• [Presentation] 血液がんに対する免疫療法の考え方-ワクチンから細胞療法へ- (2012)
  • Author(s): 藤原 弘
  • Organizer: 第5回大隅血液セミナー
  • Place of Presentation: 鹿児島県 鹿屋市
  • Invited
• [Presentation] 白血病に対する細胞免疫療法の考え方-ペプチドワクチンから細胞療法へ- (2012)
  • Author(s): 藤原 弘
  • Organizer: 第28回セルセラピーセミナー
  • Place of Presentation: 群馬県 前橋市
  • Invited
• [Presentation] Redirected T-cell based adoptive immunotherapy for human leukemia; where we are now and where we will go next.
  • Author(s): Hiroshi Fujiwara.
  • Organizer: Lecture at Hematology Branch, NHLBI/NIH
  • Place of Presentation: Hematology Branch, National Heart, Lung and Blood Institute/ NIH, Bethesda, MA
  • Invited
• [Presentation] Gene-modified CD4+ T cells using HLA class I-restricted and WT1-specific T-cell receptor gene transfer successfully enhance the antileukemia efficacy of redirected T cell-based adoptive immunotherapy
  • Author(s): Hiroshi Fujiwara.
  • Organizer: The 4th JSH (Japanese Society of Hematology) International Symposium
  • Place of Presentation: Matsuyama, Japan
  • Invited
• [Presentation] Redirected T cell-based antileukemia adoptive immunotherapy using tumor-specific TCR gene transfer.
  • Author(s): Hiroshi Fujiwara
  • Organizer: 第75回日本血液学会学術集会 Symposium4. (English Session)
  • Place of Presentation: 札幌市
  • Invited
• [Presentation] hTERTを治療標的抗原とするATLに対する新規細胞免疫療法の開発研究
  • Author(s): 藤原 弘
  • Organizer: 第2回 ATLシンポジウム／第6回HTLV-1研究会
  • Place of Presentation: 東京
  • Invited
• [Presentation] Gene-modified human α/β-T cells expressing a chimeric CD16-CD3ζ receptor as adoptively transferable effector cells for anticancer monoclonal antibody therapy
  • Author(s): Hiroshi Fujiwara
  • Organizer: 40th Annual Meeting of the European Society for Blood and Marrow Transplantation.
  • Place of Presentation: Milan, Italy
• [Presentation] Co-Administration of Gene-Modified CD4+ T Cells Targeting HLA Class I-Restricted WT1 Epitope Diversely Enhances The Antitumor Effect Mediated By Redirected T-Cell Based Adoptive Immunotherapy Against Human Leukemia.
  • Author(s): Hiroshi Fujiwara et al.
  • Organizer: 55th American Society of Hematology, Annual meeting and Exposition.
  • Place of Presentation: New Orleans, LA
• [Presentation] Generation of high-quality TCR-gene modified T cells using siTCR lentiviral vector combined with anti-CD3/CH296 stimulation and transduction method.
  • Author(s): Sachiko Okamoto et al.
  • Organizer: 16th Annual Meeting of American Society of Gene & Cell Therapy
  • Place of Presentation: Salt Lake City, UT
• [Presentation] Human telomerase reverse transcriptase‒specific T-cell receptor gene transfer redirects T cells to display an effective antitumor reactivity against adult T-cell leukemia.
  • Author(s): Hiroshi Fujiwara
  • Organizer: 39th Annual Meeting of the European Group for Blood and Marrow Transplantation.
  • Place of Presentation: London, UK
• [Presentation] 白血病細胞株K562由来人工抗原提示細胞によるT細胞抑制機能。
  • Author(s): 谷本一史 他
  • Organizer: 第18回日本がん免疫学会総会
  • Place of Presentation: 宇部市
• [Presentation] 抗体依存性細胞傷害活性（ADCC）を介した抗腫瘍効果を示す人工CD16陽性T細胞の開発。
  • Author(s): 越智史博 他
  • Organizer: 第18回日本がん免疫学会総会
  • Place of Presentation: 宇部市
• [Presentation] 経静脈的に輸注されたWT1特異的人工CTLは腎糸球体たこ足細胞を傷害しない。
  • Author(s): 朝井洋晶 他
  • Organizer: 第18回日本がん免疫学会総会
  • Place of Presentation: 宇部市
• [Presentation] 抗体療法と細胞療法を組み合わせたハイブリッド型抗腫瘍免疫療法の開発研究。
  • Author(s): 藤原 弘 他
  • Organizer: 第5回造血器腫瘍免疫療法研究会学術集会
  • Place of Presentation: 名古屋市
• [Presentation] HLA class-I restricted tumor antigen-specific CD4+ T cells for antileukemia adoptive immunotherapy.
  • Author(s): Hiroshi Fujiwara et al.
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜市
• [Presentation] Highly efficient and safe TCR gene therapy with novel siTCR vector encoding high affinity TCR variants.
  • Author(s): Sachiko Okamoto et al.
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜市
• [Presentation] Artificial CD16 positive T cells successfully display in vivo antitumor effect through ADCC.
  • Author(s): Fumihiro Ochi et al.
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜市
• [Presentation] Myeloid leukemia cell lines suppress T cell proliferation.
  • Author(s): Kazushi Tanimoto et al.
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜市
• [Presentation] Preclinical evaluation for renal safety of WT1-targeting adoptive immunotherapy.
  • Author(s): Hiroaki Asai et al.
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 札幌市
• [Presentation] Induction of WT1-specific human CD8+ T cells in an HLA class I TG NOD/SCID/Il2rgKO xenograft system.
  • Author(s): Yuho Najima et al.
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 札幌市
• [Presentation] Artificial CD16 positive T cells successfully display in vitro anti-tumor effect through ADCC.
  • Author(s): Fumihiro Ochi et al.
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 札幌市
• [Presentation] K562 based artificial antigen presenting cells inhibit T cell proliferation.
  • Author(s): Kazushi Tanimoto et al.
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 札幌市
• [Presentation] 経静脈的に輸注されたWT1特異的人工CTLは腎糸球体たこ足細胞を傷害しない。
  • Author(s): 朝井洋晶 他
  • Organizer: 第36回日本造血細胞移植学会
  • Place of Presentation: 宜野湾市
• [Presentation] キメラ型CD16遺伝子改変T細胞は抗腫瘍性ADCC活性におけるNK細胞の問題点を解決できる可能性がある。
  • Author(s): 藤原 弘 他
  • Organizer: 第36回日本造血細胞移植学会
  • Place of Presentation: 宜野湾市
• [Remarks] 愛媛大学大学院 血液・免疫・感染症内科学(第一内科) ホームページ
  • URL: http://www.m.ehime-u.ac.jp/school/int.med1/
• [Remarks] 愛媛大学大学院血液免疫感染症内科学分野ホームページ
  • URL: http://www.m.ehime-u.ac.jp/school/int.med1/
• [Remarks] 愛媛大学大学院医学系研究科医学専攻 血液・免疫・感染症内科学（第一内科）ホームページ
  • URL: http://www.m.ehime-u.ac.jp/school/int.med1/