| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Outline of Final Research Achievements |
To elucidate the autoimmunity in Beh?et's Disease (BD), we surveyed autoantigens (autoAgs) in patients with BD and investigated prevalence and clinical significance of the identified autoantibodies (autoAbs).
We prepared whole cofilin-1 and three fragments as fusion protein with maltose binding protein. The autoAbs to cofilin-1, were investigated by Enzyme-Linked ImmunoSorbent Assay using fusion protein of cofilin-1 in 60 patients with BD. The autoAbs to cofilin-1 were detected by WB in 11 (18.3%) of the 60 patients with BD. Multiple epitopes on cofilin-1 were recognized by 45.5% of the anti-cofilin-1 positive serum samples. The autoAbs to cofilin-1 are probably produced by the antigen-driven mechanism. Clinically, the existence of the autoAbs to cofilin-1 correlated with the erythrocyte sedimentation rate and production of interferon alpha.
Our data indicates that the generation of autoAbs to cofilin-1 may reflect common immunological disorders in BD.
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