| Project/Area Number |
24591495
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | Kurume University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HATA Ryou 久留米大学, 医学部, 助教 (40461404)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | インフルエンザ菌 / 感染症難治化 / 薬剤耐性 / バイオフィルム / 細胞内侵入 / BLNAR / 細胞内寄生 / 治療戦略 |
|---|
| Outline of Final Research Achievements |
Drug susceptibility testing, biofilm productions, and the intracellular invasion rate were investigated. Furthermore, human respiratory epithelial cells were previously infected by beta-lactamase-negative ampicillin-resistant Haemophilus influenzae (BLNAR) strains, and the morphological changes of BLNAR strains were observed using a focused ion beam (FIB / SEM) system after the continuous administration of cefotaxime and tosufloxacin. As a result, significant morphological changes, such as deformation and rupture were observed from not only bacteria on the surface of epithelial cells, but those invaded the cells. According to our results, a high bactericidal action of tosufloxacin has been proven, which suggested that it may become an effective agent for treating refractory pediatric otitis media.
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