| Project/Area Number |
24591565
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Tokai University (2014)
Gunma Institute of Public Health and Environmental Sciences (2012-2013) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Yoshiyuki 群馬県衛生環境研究所, 研究企画係, 研究員 (80309252)
HAYASHI Yasuhide 群馬県衛生環境研究所, 研究企画係, 研究員 (30238133)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ウイルス感染 / 好酸球 / 気管支喘息 / サイトカイン / ウイルス感染喘息 / RSウイルス / シグナル伝達 |
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| Outline of Final Research Achievements |
We investigated the effects of eosinophil granule proteins on bronchial epithelial cell infected with RS virus. Morphological changes in human type II pulmonary alveolar epithelial cells, A549 with RS virus and/or eosinophil granule proteins such as MBP, EPO, ECP, and EDN were observed by microscopy. Apoptosis/necrosis was evaluated by trypan blue exclusion test. We also measured 27 types of cytokines/chemokines production in supernatant of the cells and 12 types of phosphorylated proteins in the cells. MBP or EPO induced cytotoxicity and necrosis of A549 cells with RS virus. GM-CSF and IL-17 production was elevated in RS virus- and MBP-treated A549 cells. MBP induced the phosphorylation of ERK 1/2, p38 MAPK, JNK, and STAT 3 in A549 cells with RS virus. Eosinophil granule proteins specifically MBP damage RS virus-infected bronchial epithelial cells, indicating that eosinophilic inflammation might be associated with pathophysiology of RS virus-induced acute exacerbations of asthma.
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