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Functional analysis of Polycomb-group genes in regulating mechanisms for self-reneual and differentiation of cardiac progenitor cells.

Research Project

Project/Area Number 24591595
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pediatrics
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

SHIRAI Manabu  独立行政法人国立循環器病研究センター, 研究所, 室長 (70294121)

Co-Investigator(Renkei-kenkyūsha) MORISKI Takayuki  国立循環器病研究センター, 研究所, 部長 (30174410)
MORISKI Hiroko  国立循環器病研究センター, 研究所, 室長 (40311451)
HONDA Takehiko  国立循環器病研究センター, 研究所, 室員 (10455545)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsポリコーム遺伝子群 / 心臓発生 / 心不全 / ポリコーム遺伝子 / 心臓形態形成 / 発生医学
Outline of Final Research Achievements

To understand whether the Polycomb group (PcG) protein, Pcgf5, is implicated in maintaining balance between self-renewal and differentiation of cardiac progenitor cells, we generated Pcgf5 deficient mice. Homozygous mice were smaller than wild type. They were, however, fertile, and did not show any obvious developmental abnormalities. At around 1 year old, only homozygotes died with cardiac dysfunction. Pcgf5 deficient cardiomyocytes were hypertrophic and disorganized, however, no significant difference of cell proliferation and death between controls and homozygotes. Since 6 months old, Pcgf5 deficient males showed decrease of left ventricular ejection fraction, enlargement of the heart and upregulation of expression of Nppa/ANP, Acta1/αSK and Myh7/βMhc, known to be upregulated by cardiac overloading. These findings indicate that Pcgf5 plays a pivotal role in maintaining the cardiac function in aged mice.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (7 results)

All 2014 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] Transcription of the Geminin gene is regulated by a negative-feedback loop.2014

    • Author(s)
      Ohno, Y., Saeki, K., Yasunaga, S., Kurogi. T., Suzuki-Takedachi, K., Shirai, M., Mihara K., Yoshida, K., Voncken J. W, Ohtsubo, M. & Takihara Y.
    • Journal Title

      Mol. Biol. Cel

      Volume: 25(8) Issue: 8 Pages: 1374-1383

    • DOI

      10.1091/mbc.e13-09-0534

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Scmh1 has E3 ubiquitin ligase activity for Geminin and histone H2A and regulates Geminin stability directly or indirectly via transcription repression of Hoxa9 and Hoxb42013

    • Author(s)
      Yasunaga, S., Ohtsubo, M., Ohno, Y., Saeki, K., Kurogi. T., Tanaka-Okamoto, M., Ishizaki, H., Shirai, M., Mihara, K., Brock, H. W., Miyoshi, J. & Takihara Y
    • Journal Title

      Mol. Cell. Biol.

      Volume: 33(4) Issue: 4 Pages: 644-660

    • DOI

      10.1128/mcb.00974-12

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Functional roles of Polycomb ring finger 5 (Pcgf5) in the heart of adult mice.2014

    • Author(s)
      Shirai, M., Otani, K., Tsuchimochi, H., Takihara, Y., and Morisaki, T.
    • Organizer
      第37回日本分子生物学会
    • Place of Presentation
      神奈川県横浜市西区みなとみらい1-1-1 パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Pcgf5 contributes to PRC1 (Polycomb repressive complex 1) in developing cardiac cells.2013

    • Author(s)
      Shirai, M., Takihara, Y., Morisaki, T.
    • Organizer
      The 7th TAKAO International Symposium on Etiology and Morphogenesis of Congenital Heart Disease
    • Place of Presentation
      東京都渋谷区代々木神園町3-1 国立オリンピック記念青少年総合センター
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Functional analysis of Pcgf5 (Polycomb ring finger 5) in the developing hearts.2013

    • Author(s)
      Shirai, M., Takihara, Y., Morisaki, T.
    • Organizer
      第36回 日本分子生物学会
    • Place of Presentation
      兵庫県神戸市中央区港島中町6 神戸国際会議場、神戸国際展示場、神戸ポートピアホテル
    • Related Report
      2013 Research-status Report
  • [Presentation] 心臓発生における、ポリコーム遺伝子群の発現及び機能解析2012

    • Author(s)
      白井 学、瀧原 義宏、森崎 隆幸
    • Organizer
      第11回心臓血管発生研究会
    • Place of Presentation
      福島県郡山市
    • Related Report
      2012 Research-status Report
  • [Presentation] Functional analysis of Pcgf5 (Polycomb ring finger 5)2012

    • Author(s)
      Shirai, M., Takihara, Y., and Morisaki, T.
    • Organizer
      第 35 回日本分子生物学会
    • Place of Presentation
      福岡県福岡市
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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