Fetal gene response of maternal undernutrition mouse at ischemia reperfusion

• Project/Area Number: 24591598
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Tohoku University
• Principal Investigator: ITO Takuya 東北大学, 医学(系)研究科(研究院), 助教 (70396539)
• Co-Investigator(Kenkyū-buntansha): KIMURA Yoshitaka 東北大学, 医学研究科, 教授 (40261622) ; SATO Naoaki 東北大学, 医学研究科, 助教 (70431567) ; FUNAMOTO Kiyoe 東北大学, 医学研究科, 技術補佐員 (30570030) ; DONG Yupeng 東北大学, 医学研究科, 助手 (10569320) ; CLARISSA Velayo 東北大学, 東北メディカルメガバンク, 助教 (30635636)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 胎児脳出血 / 転写因子 / HIF1-alpha / p53 / pifithrin-alpha / 胎児医学 / DOHaD / 低酸素遺伝子応答

Outline of Final Research Achievements

The aim of this study is to find out the risk factors gene network and transcription factor for fetal cerebral hemorrhage onset. We compared the gene expression of the fetal brain of model mouse before and after ischemic-reperfusion.
Expression levels of mitochondria-associated genes were clustered in p53 expression level. By the maternal treatment of p53 inhibitor, the onset of fetal cerebral hemorrhage has been suppressed. Vulnerable to hypoxia derived from maternal malnutrition has been suggested to be involved in the aberrant activity of mitochondrial by p53 signaling activity.

Research Products

• [Journal Article] Molecular patterns of neurodevelopmental preconditioning: a study of the effects of antenatal steroid therapy in a protein-restriction mouse model (2014)
  • Author(s): Clarissa Velayo,Takuya Ito,Yupeng Dong, Miyuki Endo,RikaSugibayashi,Kiyoe Funamoto, Keita!Iida, Nobuo Yaegashi , Yoshitaka Kimura
  • Journal Title: ISRN Obstetrics and Gynecology Volume: 2014 Pages: 1-13
  • DOI: 10.1155/2014/193816
  • Peer Reviewed
• [Presentation] REPEATED ISCHEMIA REPERFUSION IN A MATERNAL PROTEIN RESTRICTION MODEL INCREASES THE RISK OF FETAL BRAIN HEMORRHAGE VIA P53-SIGNAL ACTIVATION (2013)
  • Author(s): Takuya Ito, Kenichi Funamoto, Kiyoe Funamoto, Clarissa L. Velayo, Yupeng Dong, Miyuki Endo, Rika Sugibayashi, Keita Iida, Toshiyuki Hayase, Nobuo Yaegashi Yoshitaka Kimura
  • Organizer: 8th World Congress on Developmental Origins of Health and Disease
  • Place of Presentation: Singapore, Singapore
• [Remarks] 東北大学大学院医学系研究科融合医工学分野 木村研究室
  • URL: http://www.fetalecg.med.tohoku.ac.jp/kimuralab/kimuralab_top_jn.html