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Surfactant-associated protein imaging in alveolar epithelialcells by Atmospheric Scanning Electron Microscope

Research Project

Project/Area Number 24591608
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Embryonic/Neonatal medicine
Research InstitutionKeio University

Principal Investigator

MATSUZAKI Yohei  慶應義塾大学, 医学部, 助教 (60327583)

Co-Investigator(Kenkyū-buntansha) YOSHIRO Sohma  慶應義塾大学, 医学部, 准教授 (60268183)
Co-Investigator(Renkei-kenkyūsha) MASATO Yasui  慶應義塾大学, 医学部, 教授 (90246637)
CHIKARA Sato  産業技術総合研究所, バイオメディカル研究部門, 研究グループ長 (00357146)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsサーファクタント / 慢性肺疾患 / ASEM / CARS顕微鏡 / 脂質
Outline of Final Research Achievements

We observed lamellar body which emits very strong CARS signal in alveolar type II epithelial cells by C-H mode of CARS microscope. Primary cultured alveolar type II epithelial cells on a special dish for ASEM were fixed with 4% formaldehyde, immunostained with ABCA3 antibodies and observed by ASEM. ABCA3 was observed as similar to lipid observer by CARS microscopy in alveolar type II epithelial cells. Since fixed rate of alveolar type Ⅱ epithelial cells to a dish for ASEM was low unfortunately, only limited study was performed. Finally, because sticking with a coating using a liquid collagen began to be stable, we will continue to further consideration, including the electron microscope observations.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (3 results)

All 2014 2013

All Journal Article (2 results) Presentation (1 results)

  • [Journal Article] 肺血管発生から肺循環の適応へ2014

    • Author(s)
      松崎陽平, 池田一成
    • Journal Title

      心臓

      Volume: 46 Pages: 166-169

    • NAID

      130004716916

    • Related Report
      2013 Research-status Report
  • [Journal Article] 肺の発生発達の分子メカニズム2013

    • Author(s)
      松崎陽平, 池田一成, 佐治勉
    • Journal Title

      Fetal & Neonatal Medicine

      Volume: 5 Pages: 101-102

    • Related Report
      2013 Research-status Report
  • [Presentation] Coherent Anti-Stokes Raman Scattering顕微鏡によるll型上皮細胞におけるサーフ ァクタントの動態観察2013

    • Author(s)
      松崎陽平, 池田一成
    • Organizer
      第49回日本周産期・新生児医学会学術集会
    • Place of Presentation
      神奈川県横浜 パシフィコ横浜
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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