Establishment of extramammary Paget's disease derived cell line: Experimental research of the effect of anti-androgen reagents on extramammary Paget's disease cell line.

• Project/Area Number: 24591640
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Kurume University
• Principal Investigator: KARASHIMA Tadashi 久留米大学, 医学部, 講師 (70211175)
• Co-Investigator(Kenkyū-buntansha): HASHIMOTO Takashi 久留米大学, 皮膚細胞生物学研究所, 教授 (20129597) ; FURUMURA Minao 久留米大学, 医学部, 准教授 (10315070)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 乳房外ページェット病 / 乳房外ページェット病由来細胞株 / 男性ホルモン / 男性ホルモンレセプター / EGF / アンドロゲン受容体 / 皮膚腫瘍 / 乳房外ページッェット病

Outline of Final Research Achievements

We established Extramammary Paget’s disease cell line (EMPE cell), and characterized their growth behavior. EMPE cells expressed androgen receptor (AR) and androgen enhanced their growth. EGF also enhanced their growth, but EGF receptor was not expressed in EMPE cell. It is suggested EGF may play a role of ligand –independent of AR activation pathway in EMPE cell.