Regulation of skin immunity by regulatory T cell-subset

• Project/Area Number: 24591649
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Kyoto University
• Principal Investigator: NOMURA Takashi 京都大学, 医学(系)研究科(研究院), その他 (60346054)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 皮膚免疫学 / 免疫制御 / アレルギー / 免疫寛容 / 皮膚免疫 / 制御性T細胞 / 免疫トレランス / Helios

Outline of Final Research Achievements

Although etiology of atopic dermatitis, psoriasis, and collagen diseases is not fully clarified, it is partly explained by excessively activated immune system. Such over-activation is inhibited by special lymphocytes called regulatory T cells. In this project, we developed Helios-reporter mice, in which Helios-expressing cells expressed fluorescent proteins; and we analyzed live regulatory T cells expressing Helios. Our study revealed that Helios-expressing regulatory T cells possessed a strong suppressive activity. Helios-reporter mice are applicable for developing measures to restrain excessive immune responses or to treat allergic and autoimmune diseases.

Research Products

• [Journal Article] Generation of Helios reporter mice and an evaluation of the suppressive capacity of Helios+ regulatory T cells in vitro (2015)
  • Author(s): Sugita K., Hanakawa S., Honda T., Kondoh G., Miyachi Y., Kabashima K., Nomura T.
  • Journal Title: Experimental Dermatology Volume: Ahead of print Issue: 7 Pages: 554-556
  • DOI: 10.1111/exd.12711
  • NAID: 120005674664
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Eosinophilic pustular folliculitis: The transition in sex differences and interracial characteristics between 1965 and 2013 (2015)
  • Author(s): Nomura T, Katoh M, Yamamoto Y, Kabashima K, Miyachi Y.
  • Journal Title: J Dermatol Volume: in press Issue: 4 Pages: 343-352
  • DOI: 10.1111/1346-8138.12783
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The panoply of abT cells in the skin (2014)
  • Author(s): Nomura T., Kabashima K., Miyachi Y.
  • Journal Title: Journal of Dermatological Science Volume: 76 Issue: 1 Pages: 3-9
  • DOI: 10.1016/j.jdermsci.2014.07.010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] TGF-b under non-inflammatory cytokine milieu converts naive CD4+ T cells into thymic-type Helios+ regulatory T cells (2013)
  • Author(s): Takashi Nomura, Kazunari Sugita, Norihito Hayatsu, Akihiko Kitoh, Yoshiki Miyachi, and Kenji Kabashima
  • Organizer: International Investigative Dermatology Edinburgh 2013
  • Place of Presentation: Edinburgh, UK
• [Presentation] TGF-b under non-inflammatory cytokine milieu converts naive CD4+ T cells into thymic-type Helios+ regulatory T cells. (2013)
  • Author(s): Takashi Nomura, Kazunari Sugita, Norihito Hayatsu, Akihiko Kitoh, Yoshiki Miyachi, and Kenji Kabashima
  • Organizer: International Investigative Dermatology Edinburgh 2013
  • Place of Presentation: Edinburgh, U.K.
• [Presentation] Visualization of Helios-expressing T cells in the skin. (2012)
  • Author(s): Nomura T., Kabashima K., Miyachi Y.
  • Organizer: The American Association of Immunologists Annual Meeting
  • Place of Presentation: Boston, U.S.A.
• [Presentation] Stimulation with anti-CD3/CD28 beads and TGF-β in the non-inflammatory milieu converts CD4 + T cells into Helios + Foxp3 + regulatory T cells. (2012)
  • Author(s): Nomura T., Hayatsu N., Sugita K., Kabashima K., Miyachi Y.
  • Organizer: The Japanese Society for Investigative Dermatology Annual Meeting
  • Place of Presentation: Okinawa, Japan