| Project/Area Number |
24591671
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Fujita Health University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAGAMI Akiko 藤田保健衛生大学, 医学部, 准教授 (90367699)
SANO Akiyo 藤田保健衛生大学, 医学部, 助教 (20625983)
NAKAMURA Masashi 藤田保健衛生大学大学院, 医学研究科 (30449467)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 加水分解コムギ / グルパール１９S / 即時型コムギアレルギー / 特異IgE抗体 / 検査法 / 抗原解析 / 交叉反応性 / 予後因子の探索 / グルパール19S / 経皮膚・粘膜感作 / 小麦アレルギー / 交差反応性 / 診断方法の構築 / 予後因子探索 / 重症度分類 / ELISA法 |
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| Outline of Final Research Achievements |
The purpose of this study was to elucidate the pathologic conditions and to establish the diagnostic methods in hydrolyzed wheat protein- induced immediate-type wheat allergy (HWP-IWA) . Our findings show that the ELISA-based GP19S specific IgE assay is a useful quantitative and high throughput method for the diagnosis. Using ELISA, skin prick test and Western blotting, we elucidated that patients who developed an allergy to GP19S also had cross-reactivity to some other HWPs. This suggested that other HWPs might contain the epitope sequences in GP19S. On the other hands, the GP19S antigen was thought to be generated after gluten was exposed to hydrochloric acid and heated to 95 °C. The heating time was long enough to initiate deamidation but too short to initiate a change in molecular weight, and precipitation at the isoelectric point resulted in an increase in antigen concentration.
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