Functional role of the N-terminal domain of DFosB in response to stress and drugs of abuse.

• Project/Area Number: 24591735
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Psychiatric science
• Research Institution: Kurume University
• Principal Investigator: OHNISHI Yoshinori 久留米大学, 医学部, 助教 (10626865)
• Co-Investigator(Kenkyū-buntansha): NISHI Akinori 久留米大学, 医学部, 教授 (50228144)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: うつ病 / コカイン依存症 / FosB / 転写因子 / 行動実験 / 側坐核 / AP-1 / 依存症 / コカイン / 薬物依存症 / fosB / AAV / 遺伝子改変マウス / 国際情報交換 / 米国ニューヨーク

Outline of Final Research Achievements

DFosB is transcriptional factor, which is accumulated in specific brain regions by chronic or repeated stimulation of stress or addictive drugs. We have reported DFosB makes a role for anti-depressant. However, overexpressed DFosB mRNA codes DFosB and N-terminal lacking D2DFosB, and expresses at similar level. We tried to reveal which one has more important role for depression and cocaine addiction by respective expression using AAV in nucleus accumbens. In results, DFosB enhanced cocaine addiction after social defeat stress, and works as anti-depressant, but, D2DFosB inhibit such type of cocaine addiction and does not work as anti-depressant.

Research Products

• [Journal Article] PSMC5, a 19S Proteasomal ATPase, Regulates Cocaine Action in the Nucleus Accumbens. (2015)
  • Author(s): Ohnishi YH, Ohnishi YN, Nakamura T, Ohno M, Kennedy PJ, Yasuyuki O, Nishi A, Neve R, Tsuzuki T, Nestler EJ.
  • Journal Title: PLoS One Volume: 10(5) Issue: 5 Pages: e0126710-e0126710
  • DOI: 10.1371/journal.pone.0126710
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Functional role of the N-terminal domain of ΔFosB in response to stress and drugs of abuse. (2015)
  • Author(s): Ohnishi YN, Ohnishi YH, Vialou V, Mouzon E, LaPlant Q, Nishi A, Nestler EJ.
  • Journal Title: Neuroscience Volume: 22;284 Pages: 165-70
  • DOI: 10.1016/j.neuroscience.2014.10.002
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Fosb gene products contribute to excitotoxic microglial activation by regulating the expression of complement C5a receptors in microglia. (2014)
  • Author(s): Nomaru, H., Sakumi, K., Katogi, A., Ohnishi, Y.N., Kajitani, K., Tsuchimoto, D., Nestler, E.J., and Nakabeppu, Y.
  • Journal Title: Glia Volume: N/A Issue: 8 Pages: 1284-1298
  • DOI: 10.1002/glia.22680
  • Peer Reviewed
• [Journal Article] fosB-Null Mice Display Impaired Adult Hlppocampal Neurogenesis and Spontaneous Epilepsy (2013)
  • Author(s): Yutsudo, N, et. al.
  • Journal Title: Neuropsychopharmacology Volume: 38 Issue: 5 Pages: 895-906
  • DOI: 10.1038/npp.2012.260
  • Peer Reviewed
• [Journal Article] BDNF is a negative modulator of morphine action. (2012)
  • Author(s): Koo JW, Mazei-Robison MS, Chaudhury D, LaPlant Q, Ferguson D, Feng J, Sun H, Scobie KN, Ohnishi YN, Ohnishi YH, Mouzon E, Lobo MK, Neve RL, Russo SJ, Han MH, Nestler EJ et. al.
  • Journal Title: Science Volume: 338 Issue: 6103 Pages: 124-128
  • DOI: 10.1126/science.1222265
  • Peer Reviewed
• [Presentation] Functional role of the N-terminal domain of deltaFosB in responses to stress and drugs of abuse (2013)
  • Author(s): Y. N. OHNISHI, Y. H. OHNISHI, V. VIALOU, E. MOUZON, Q. LAPLANT, A. NISHI, E. J. NESTLER
  • Organizer: Neuroscience 2013
  • Place of Presentation: San Diego
• [Presentation] Functional role of the N-terminal domain of deltaFosB in responses to stress and drugs of abuse (2013)
  • Author(s): Y. N. OHNISHI, Y. H. OHNISHI, V. VIALOU, E. MOUZON, Q. LAPLANT, A. NISHI, E. J. NESTLER
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Functional role of the N-terminal domain of deltaFosB in responses to stress and drugs of abuse (2012)
  • Author(s): Y. N. OHNISHI, Y. H. OHNISHI, V. VIALOU, E. MOUZON, Q. LAPLANT, A. NISHI, E. J. NESTLER
  • Organizer: Neuroscience 2012, SfN's 42nd annual meeting
  • Place of Presentation: New Orleans Convention center
• [Presentation] Functional role of the N-terminal domain of deltaFosB in responses to stress and drugs of abuse (2012)
  • Author(s): Y. N. OHNISHI, Y. H. OHNISHI, V. VIALOU, E. MOUZON, Q. LAPLANT, A. NISHI, E. J. NESTLER
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Remarks] 久留米大学医学部薬理学講座のホームページ
  • URL: http://www.med.kurume-u.ac.jp/med/pharm/
• [Remarks] 久留米大学医学部薬理学講座
  • URL: http://www.med.kurume-u.ac.jp/med/pharm/