Preclinical PET imaging for the daignosis and treatment of pancreatic tumor model

• Project/Area Number: 24591804
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: Aung U.Winn 国立研究開発法人放射線医学総合研究所, 分子イメージング研究センター, 主任研究員 (70392214)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 膵臓癌 / 同所移植癌モデル / 核医学イメージング（PETを含む） / 同所移植膵臓癌モデル / ＰＥＴイメージング / マルチモーダルイメージング

Outline of Final Research Achievements

The purpose of our study is to develop a clinically relevant orthotopic xenotransplantation model of pancreatic cancer that reflects the clinical situation and, to perform the preclinical evaluation of new diagnostic and therapeutic PET imaging probes, allowed in this model. At first, we succeeded to establish the orthotopic pancreatic cancer xenograft model. Next, among three candidates of PET imaging probes (64Cu-RAFT-RGD, 11C-Sorafenib, 11C-DAC), we put focus on the 64Cu-RAFT-RGD probe and performed in vitro and in vivo experiments, small animal 64Cu-RAFT-RGD PET imaging comparing with 18F-FDG-PET, contrasted CT (CECT) after PET, and examined the potential applicability of probe. Postimaging autoradiography, histologic, immunohistochemical and immunofluorescence examinations, and the comprehensive results suggested that 64Cu-RAFT-RGD possesses the specific binding to αvβ3 integrin of pancreatic tumor cells and may provide higher detectability over 18F-FDG-PET.

Research Products

• [Journal Article] Radioimmunotherapy of pancreatic cancer xenografts in nude mice using 90Y-labeled anti-α6β4 integrin antibody. (2016)
  • Author(s): 1.Winn Aung, Atsushi B.Tsuji, Hitomi Sudo, Aya Sugyo, Yoshinori Ukai, Katsushi Kouda, Yoshikazu kurozawa, Takako Furukawa, Tsuneo Saga
  • Journal Title: Oncotarget Volume: 7
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Immunotargeting of integrin α6β4 for single-photon emission computed tomography and near-infrared fluorescence imaging in a pancreatic cancer model (2016)
  • Author(s): 2.Winn Aung, Atsushi B.Tsuji, Hitomi Sudo, Aya Sugyo, Takako Furukawa, Yoshinori Ukai, Yoshikazu kurozawa, Tsuneo Saga
  • Journal Title: Molecular Imaging Volume: 15
  • DOI: 10.1177/1536012115624917
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Micro-Positron Emission Tomography/Contrast-Enhanced Computed Tomography Imaging of Orthotopic Pancreatic Tumor-Bearing Mice Using the αvβ3 Integrin Tracer 64Cu-Labeled Cyclam-RAFT-c(-RGDfK-)4 (2013)
  • Author(s): U Winn Aung, Zhao-Hui Jin, Takako Furukawa, Michael Claron, Didier Boturyn, Chizuru Sogawa, Atsushi Tsuji, Hidekatsu Wakizaka, Toshimitsu Fukumura, Yasuhisa Fujibayashi, Pascal Dumy, Tsuneo Saga
  • Journal Title: Molecular Imaging Volume: 12(6) Issue: 6 Pages: 376-387
  • DOI: 10.2310/7290.2013.00054
  • Peer Reviewed
• [Presentation] Study for single and fractionated radioimmunotherapy (RIT) of 90Y-labeled anti-α6β4 integrin antibody in pancreatic cancer model (2015)
  • Author(s): Winn Aung, Atsushi Tsuji, Hitomi Sudo, Aya Sugyo, Takako Furukawa, Yoshinori Ukai, Yoshikazu Kurosawa, Tsuneo Saga
  • Organizer: 2015 The 8th World Molecular Imaging Congress
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2015-09-02
  • Int'l Joint Research
• [Presentation] U Winn Aung, Atsushi B. Tsuji, Hitomi Sudo, Aya Sugyo, Takako Furukawa, Yoshinori Ukai, Yoshikazu Kurosawa, Tsuneo Saga: Integrin α6β4-targeted near-infrared imaging and single-photon emission computed tomography in a pancreatic cancer model (2014)
  • Author(s): Integrin α6β4-targeted near-infrared imaging and single-photon emission computed tomography in a pancreatic cancer model
  • Organizer: 2014 World Molecular Imaging Congress
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2014-09-17 – 2014-09-20
• [Presentation] 同所移植膵臓癌モデルを用いた診断を目指す64Cu-RAFT-RGD PET/造影CTイメージング (2013)
  • Author(s): U Winn Aung, 金 朝暉, 古川 高子, クラホン ミチャエル, ボチュン ディテェル, 曽川 千鶴, 辻 厚至, 藤林 康久, デュミ パスカル, 佐賀 恒夫
  • Organizer: 日本核医学会
  • Place of Presentation: 福岡,日本
• [Presentation] MicroPET/CT imaging of orthotopic pancreatic tumor-bearing mice using the integrin tracer 64Cu-labeled cyclam-RAFT-c(-RGDfK-)4 peptide (2012)
  • Author(s): U Winn Aung, Zhao-Hui Jin, Takako Furukawa, Michael Claron, Didier Boturyn, Chizuru Sogawa, Yasuhisa Fujibayashi, Pascal Dumy and Tsuneo Saga
  • Organizer: 2012 World Molecular Imaging Congress
  • Place of Presentation: Dublin, Ireland