The research for the mechanisms and protection method of radiation-induced suppression of myotube formation

• Project/Area Number: 24591839
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Gihu University of Medical Science
• Principal Investigator: SAKURAI Tomonori 岐阜医療科学大学, 保健科学部, 教授 (90400142)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 筋芽細胞 / 筋管形成 / 筋管形成阻害 / 増殖因子 / IGF-1 / FGF-2 / HGF / C2C12

Outline of Final Research Achievements

I investigated the mechanisms and the protection methods of X-ray-induced delay of myotube formation. I evaluated the alterations of gene expressions of four key transcription factors and demonstrated the decrease in myogenin gene expression related to the X-ray-induced decrease in myotube formation. I also find that the cell cycle distributions and cell growth state of C2C12 cells affected the effects of X-ray irradiation on myotube formation. In addition, I showed that fibroblast growth factor and/or hepatocyte growth factor had the ability to protect the X-ray-induced decrease in myotube formation if these growth factors appropriately used in the point of concentration and/or time point, and so on.

Research Products

• [Journal Article] Alteration of gene expression by exposure to a magnetic field at 23 kHz is not detected in astroglia cells (2013)
  • Author(s): Sakurai T, Narita E, Shinohara N, Miyakoshi J.
  • Journal Title: J Radiat Res. Volume: 54 Issue: 6 Pages: 1005-1009
  • DOI: 10.1093/jrr/rrt063
  • NAID: 120005353508
  • Peer Reviewed
• [Journal Article] Myotube orientation using strong static magnetic fields (2012)
  • Author(s): Tomoniri Sakurai
  • Journal Title: Bioelectromagnetics Volume: 33 Issue: 5 Pages: 421-427
  • DOI: 10.1002/bem.21701
  • Peer Reviewed
• [Presentation] Gene expression analysis of radiation-induced delay of myotube formation (2015)
  • Author(s): Sakurai T, Miura S
  • Organizer: 15th International Congress of Radiation Research
  • Place of Presentation: 京都府京都市国立京都国際会館
  • Year and Date: 2015-05-25 – 2015-05-29
• [Presentation] 細胞浸潤に対する強定常磁場の影響 (2014)
  • Author(s): 櫻井智徳, 長元志高, 廣田憲之
  • Organizer: 第9回日本磁気科学会
  • Place of Presentation: 岐阜県高山市高山市民文化会館
  • Year and Date: 2014-11-13 – 2014-11-14
• [Presentation] 放射線誘発筋管形成阻害における遺伝子発現変化 (2014)
  • Author(s): 櫻井智徳、三浦沙也佳
  • Organizer: 第57回放射線影響学会大会
  • Place of Presentation: 鹿児島県鹿児島市かごしま県民交流センター
  • Year and Date: 2014-10-01 – 2014-10-03
• [Presentation] The effects of static magnetic fields on cell migration (2014)
  • Author(s): Sakurai T, Nagamoto Y, Nakahara Y, Hirota N
  • Organizer: 6th International Workshop on Materials Analysis and Processing in Magnetic Fields
  • Place of Presentation: 沖縄県糸満市
  • Year and Date: 2014-07-08 – 2014-07-11
• [Presentation] 強定常磁場の細胞影響について (2012)
  • Author(s): 櫻井 智徳
  • Organizer: 日本磁気学会第21回強磁場応用専門研究会
  • Place of Presentation: 大阪大学
  • Invited
• [Presentation] Strong static magnetic fields induced orientation of myotubes differentiated from myoblasts
  • Author(s): Sakurai T, Hirota N
  • Organizer: 3rd International Colloquium on Electric and Magnetic Fields at Extremely Low Frequencies (EMF-ELF 2013)
  • Place of Presentation: 奈良県新公会堂
• [Presentation] Strong static magnetic fields-induced alignment of myotubes
  • Author(s): Sakurai T, Hirota N
  • Organizer: International Conference on Magneto-Science
  • Place of Presentation: Bordeaux, France
• [Presentation] γ線誘発筋形成阻害に対する増殖因子の影響
  • Author(s): 櫻井智徳, 新田宗之, 尾関亨斗
  • Organizer: 第56回日本放射線影響学会
  • Place of Presentation: ホテルクラウンパレス青森
• [Book] Cellular Response to Physical Stress and Therapeutic Applications (2013)
  • Author(s): Kondo T, Matsumoto H, Sakurai T, Szasz A, Yoshihisa Y, Shimizu T, Kitaichi N, Tabuchi Y, Takahashi A, Mori Eiichiro, Ohnishi T, Hassan MA, Feril LB Jr, Tachibana K
  • Total Pages: 204
  • Publisher: Nova Biomedical