Reprograming of mouse acini induced by EGFR receptor ligands

• Project/Area Number: 24591893
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Clinical research Center, Chiba-East National Hospital, National Hospital Organization
• Principal Investigator: ASANO Takehide 独立行政法人国立病院機構(千葉東病院臨床研究センター), その他部局等, その他 (80143311)
• Co-Investigator(Kenkyū-buntansha): 鈴木 一史 千葉大学, 医学部附属病院, 助教 (00586737) ; 剣持 敬 藤田保健衛生大学, 医学部, 教授 (50215133)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 再生医療 / 幹細胞 / 可塑性 / 遺伝子発現 / リプログラミング

Outline of Final Research Achievements

Beta-cell replacement is a promising therapy for type 1 diabetes. But shortage of pancreas donor is serious problem in Japan . Many reports has been existed about success of induction beta-cell from embryonic stem cells or induced pluripotent stem cells (iPS), however risk of teratoma formation is obstacle for clinical application.
Here we show EGFR receptor ligands can dedifferentiate pancreatic acini derived from adult mice. Nestin positive cells can be obtained and they show stem cell like characters.

Research Products

• [Presentation] 膵・膵島移植研究会 (2014)
  • Author(s): 吉田徳子
  • Organizer: 第41回日本膵・膵島移植研究会
  • Place of Presentation: 名古屋ミッドランドホール
• [Presentation] 遺伝子導入のない成熟膵腺房細胞のリプログラミング (2012)
  • Author(s): 鈴木 一史
  • Organizer: 臓器保存生物医学会
  • Place of Presentation: コラッセ福島
  • Invited