The effect and mechanism of Rho kinase inhibitors on small bowel reperfusion injury

Research Project

Project/Area Number	24591968
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Digestive surgery
Research Institution	Gunma University
Principal Investigator	TAKEYOSHI IZUMI 群馬大学, 医学(系)研究科(研究院), 教授 (60272233)
Co-Investigator(Kenkyū-buntansha)	TAKAHASHI Norifumi 群馬大学, 医学部附属病院, 助教 (10599541) SUNOSE Yutaka 群馬大学, 医学部附属病院, 助教 (90375543)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000) Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	虚血再灌流障害 / 小腸 / Rhoキナーゼ / nitric oxide / nitoric oxide
Outline of Final Research Achievements	We have investigated the effect of Rho kinase inhibitors on small bowel reperfusion injury. Administration of Rho kinase inhibitor significantly increased blood flow of the SMA and the mucous membrane of the small bowel at 12 hours after reperfusion, when compared to administration of normal saline. This effect was also confirmed by histology. Rho kinase is known to down-regulate the expression of eNOS and to up-regulate the expression of MCP-1. To investigate the underlying mechanism of Rho kinase inhibitor on reduction of reperfusion injury, we examined the levels of eNOS expression by western blot analysis, however this did not show positive results. We are further examining eNOS and MCP-1 expression by immunohistochemical and PCR analysis.