Elucidation of the graft senescence after pediatric living donor liver transplantatin by telomere length and telomerase using Q-FISH method.

• Project/Area Number: 24592013
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Nippon Medical School
• Principal Investigator: Kawano Youichi 日本医科大学, 医学部, 助教 (50366671)
• Co-Investigator(Kenkyū-buntansha): Sanada Yukihiro 自治医科大学, 医学部, 助教 (60406113)
• Co-Investigator(Renkei-kenkyūsha): Takubo Kaiyo 東京都長寿医療センター, 東京都健康長寿医療センター研究所, 研究部長 (00154956)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 肝細胞テロメア長 / 小児生体肝移植 / ドナー肝 / グラフト肝 / 加齢変化 / 生体肝移植 / 小児 / ドナー / レシピエント / テロメア長 / 肝細胞 / グラフト加齢 / テロメア / テロメラーゼ

Outline of Final Research Achievements

This pilot study indicates that donor age is a crucial factor affecting telomere length sustainability in hepatocytes after pediatric LDLT, and that the telomeres in grafted livers may be elongated somewhat longer when the grafts are immunologically well controlled.

Research Products

• [Journal Article] COMPLEMENTARY INDICATORS FOR DIAGNOSIS OF HEPATIC VEIN STENOSIS AFTER PEDIATRIC LIVING DONOR LIVER TRANSPLANTATION (2016)
  • Author(s): Kawano Youichi
  • Journal Title: Transplant Proc Volume: S0041 Pages: 1156-1161
  • DOI: 10.1016/j.transproceed.2015.12.114
  • Peer Reviewed / Open Access
• [Journal Article] Donor age and operational tolerance in living donor liver transplantation. (2015)
  • Author(s): Sanada Y, Kawano Y, Ishikawa N, Aida J, Nakamura K, Izumiyama-Shimomura N, Urahashi T, Ihara Y, Okada N, Yamada N, Hirata Y, Takubo K, Mizuta K
  • Journal Title: Pediatr Transplant. Volume: 19 Pages: 244-245
  • DOI: 10.1111/petr.12431
  • Peer Reviewed / Open Access
• [Journal Article] Q-FISH measurement of hepatocyte telomere lengths in donor liver and graft after pediatric living-donor liver transplantation: Donor age affects telomere length sustainability. (2014)
  • Author(s): Kawano Y., Ishikawa N., Aida J., Sanada Y., Izumiyama-Shimomura N., Nakamura K., Poon SSS., Matsumoto K., Mizuta K., Uchida E., Tajiri T., Kawarasaki H., Takubo K.
  • Journal Title: PLoS One Volume: 9 Pages: 1-10
  • DOI: 10.1371/journal.pone.0093749
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Maternal grafts protect daughter recipients from acute cellular rejection after pediatric living donor liver transplantation for biliary atresia. (2014)
  • Author(s): Sanada Y, Kawano Y, Miki A, Aida J, Nakamura K, Izumiyama NS, Ishikawa N, Arai T, Hirata Y, Yamada N, Okada N, Wakiya T, Ihara Y, Urahashi T, Yasuda Y, Takubo K.
  • Journal Title: Transpl Int. Volume: 27 Pages: 383-390
  • DOI: 10.1111/tri.12273
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The role of operative intervention in management of congenital extrahepatic portosystemic shunt. (2012)
  • Author(s): Sanada Y, Urahashi T, Ihara Y, Wakiya T, Okada N, Yamada N, Egami S, Hishikawa S, Kawano Y, Ushijima K, Otomo S, Sakamoto K, Nakata M, Yasuda Y, Mizuta K.
  • Journal Title: Surgery Volume: 151(3) Pages: 404-11
  • Peer Reviewed
• [Journal Article] Non-alcoholic steatohepatitis caused by malnutrition after pediatric liver transplantation. (2012)
  • Author(s): Sanada Y, Urahashi T, Wakiya T, Okada N, Hishikawa S, Kawano Y, Ushijima K, Otomo S, Sakamoto K, Mizuta K.
  • Journal Title: Pediatr Int. Volume: 53(6) Pages: 1077-81
  • Peer Reviewed
• [Journal Article] Hepatocellular telomere length in biliary atresia measured by Q-FISH. (2012)
  • Author(s): Sanada Y, Aida J, Kawano Y, et al.
  • Journal Title: World J Surg. Volume: 36(4) Pages: 908-16
  • Peer Reviewed
• [Presentation] COMPLEMENTARY INDICATORS FOR DIAGNOSIS OF HEPATIC VEIN STENOSIS AFTER PEDIATRIC LIVING DONOR LIVER TRANSPLANTATION (2015)
  • Author(s): Kawano Youichi
  • Organizer: 14th Congress of the Asian Society of Transplantation
  • Place of Presentation: Singapore
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] Q-FISH measurement of hepatocyte telomere lengths in donor liver and graft after pediatric living-donor liver transplantation: donor age affects telomere length sustainability. (2015)
  • Author(s): Kawano Youichi
  • Organizer: 14th Congress of the Asian Society of Transplantation
  • Place of Presentation: Singapore
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] 胆道閉鎖症肝のテロメア長と細胞老化関連β-galactosidaseの解析 (2012)
  • Author(s): 眞田 幸弘, 相田 順子, 川野 陽一, 仲村 賢一, 泉山 七生貴, 石川 直, 新井 冨生, 山田 直也, 岡田 憲樹, 脇屋 太一, 井原 欣幸, 浦橋 泰然, 水田 耕一, 田久保 海誉
  • Organizer: 第101回日本病理学会
  • Place of Presentation: 東京
• [Remarks] 東京都健康長寿医療センター研究所 高齢者がん研究グループのホームページ
  • URL: http://www.ttaggg-rtgp.org/