Detection of pancreatic cancer by serum microRNA

• Project/Area Number: 24592039
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Tokyo Medical University
• Principal Investigator: TSUCHIDA Akihiko 東京医科大学, 医学部, 教授 (50207396)
• Co-Investigator(Kenkyū-buntansha): KURODA Masahiko 東京医科大学, 医学部, 教授 (80251304) ; YUICHI Nagakawa 東京医科大学, 医学部, 講師 (20349484)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 膵癌 / microRNA / 早期診断 / 膵がん / 血清miRNA / 分泌型miRNA / 消化器がん

Outline of Final Research Achievements

We analyzed the serum microRNA in patients with pancreatic cancer by microarray. The results showed that hsa-miR-188-5p, hsa-miR-1275 and hsa-miR-3648 were significantly higher in pancreatic cancer patients than those in healthy volunteer or patients with benign pancreatic disorders. Especially, miR-1275 increased 3.6 times in average. In future, we have a plan to analyze the role and function of these microRNA in pancreatic cancer.

Research Products

• [Journal Article] Immaturity of smooth muscle cells in the neointima is associated with acute coronary syndrome. (2015)
  • Author(s): Horita A, Kurata A, Ohno S, Shimoyamada H, Saito I, Kamma H, Kuroda M.
  • Journal Title: Cardiovasc Pathol Volume: 24 Issue: 1 Pages: 26-32
  • DOI: 10.1016/j.carpath.2014.09.003
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of the Human Papillomavirus mRNA Test for the detection of cervical lesions in Japan. (2015)
  • Author(s): Nakayama Y, Yamada M, Kurata A, Kiseki H, Kuroda M.
  • Journal Title: Eur J Gynaec Oncol Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Unique expression pattern of viral proteins in human herpesvirus 8-positive plasmablastic lymphoma: a case report. (2014)
  • Author(s): 2.Yamada M, Katano H, Yotsumoto M, Hashimoto H, Muramatsu T, Shiotsuka M, Fukutake K, Kuroda M.
  • Journal Title: Int J Clin Exp Pathol Volume: 7 Pages: 6415-6418
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Young-age-onset pancreatoduodenal carcinoma in Shwachman-Diamond syndrome. (2014)
  • Author(s): Nakaya T, Kurata A, Hashimoto H, Nishimata S, Kashiwagi Y, Fujita K, Kawashima H, Kuroda M.
  • Journal Title: Pathol Int Volume: 64 Issue: 2 Pages: 75-80
  • DOI: 10.1111/pin.12133
  • Peer Reviewed
• [Journal Article] Unique expression pattern of viral proteins in human herpesvirus 8-positive plasmablastic lymphoma: a case report. (2014)
  • Author(s): Yamada M, Katano H, Yotsumoto M, Hashimoto H, Muramatsu T, Shiotsuka M, Fukutake K, Kuroda M.
  • Journal Title: Int J Clin Exp Pathol. Volume: 7 Pages: 6415-6418
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Multifocal Kaposiform Hemangioendothelioma in Multiple Visceral Organs: An Autopsy of 9-Day-Old Female Baby. (2014)
  • Author(s): Takeo Nakaya, Kiyoko Morita, Atsushi Kurata,Tetsuo Ushiku, Takashi Igarashi, Masahiko Kuroda, Masashi Fukayama.
  • Journal Title: Human Pathology. Volume: 45 Issue: 8 Pages: 1773-1777
  • DOI: 10.1016/j.humpath.2014.03.019
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] KLF5 Regulates the Integrity and Oncogenicity of Intestinal Stem Cells (2014)
  • Author(s): Nakaya, T
  • Journal Title: Cancer Res Volume: 74 Issue: 10 Pages: 2882-2891
  • DOI: 10.1158/0008-5472.can-13-2574
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] SMAD2 MEDIATES DENDRITIC CELL SUPPRESSION BY TGF-β Smad2 is the TGF-β receptor-regulated Smad to suppress the immunogenicity of mouse and human dendritic cells. (2014)
  • Author(s): Jeong-Hwan Yoon, Katsuko Sudo, Jin Soo Han, Min-Young Chae, Seok Hee Park, Michael Weinstein, Susumu Nakae, Tadashi Yamashita, In-Kyu Lee, Takayuki Sumida, Masahiko Kuroda, and Mizuko Mamura.
  • Journal Title: European Journal of Immunology-2 Volume: in press
  • Peer Reviewed
• [Journal Article] Estrogen and estrogen receptor induce matrix metalloproteinase (MMP)-26 expression in endometrial carcinomacells (2013)
  • Author(s): Nishi H, Kuroda M, Isaka K
  • Journal Title: Oncol Rep Volume: 30(2) Issue: 2 Pages: 751-6
  • DOI: 10.3892/or.2013.2527
  • Peer Reviewed
• [Journal Article] Correlation between number of renal cysts and aortic circumferences measured using autopsy material. (2013)
  • Author(s): Kurata A, Inoue S, Ohno S, Nakatsubo R, Takahashi K, Ito T, Kawasaki K, Kokubo R, Sakai T, Ubukata J, Matsubara S, Muraoka R, Yamazaki J, Hirose T, Hojo M, Watanabe E, Kuroda M.
  • Journal Title: Pathol Res Pract Volume: 209 Issue: 7 Pages: 441-447
  • DOI: 10.1016/j.prp.2013.04.012
  • Peer Reviewed
• [Journal Article] Activin receptor-like kinase5 inhibition suppresses mouse melanoma by ubiquitin degradation of Smad4, thereby derepressing eomesodermin in cytotoxic T lymphocytes (2013)
  • Author(s): Yoon JH, Sudo K, Kuroda M. (18 名 15 番目)
  • Journal Title: EMBO molecular Medicine Volume: 5(11) Issue: 11 Pages: 1720-39
  • DOI: 10.1002/emmm.201302524
  • Peer Reviewed
• [Presentation] Development of novel form of mimic microRNA for lung cancer therapy. (2015)
  • Author(s): Ohno S, Itano K, Harada Y, Asada K, Oikawa K, Kashiwazako M, Takanashi M, Sudo K, Ikeda N, Kuroda M.
  • Organizer: AACR Annual Meeting 2015
  • Place of Presentation: Philadelphia, Pennsylvania, USA
  • Year and Date: 2015-04-18 – 2015-04-22
• [Presentation] 分泌型microRNAを用いた膵癌診断の開発 (2014)
  • Author(s): 許 文聰、黒田 雅彦、上田 しのぶ、田子 友哉、西村 絵美、中島 哲史、瀧下 智恵、佐原 八束、細川 勇一、永川 裕一、粕谷 和彦、土田 明彦
  • Organizer: 第７６回日本臨床外科学会総会
  • Place of Presentation: 郡山
  • Year and Date: 2014-11-20 – 2014-11-22
• [Presentation] The development of short form of mimic microRNA for lung cancer therapy. (2014)
  • Author(s): Kuroda M.
  • Organizer: 26th EORTC-NCI-ACR Symposium.
  • Place of Presentation: Barcelona, SPAIN.
  • Year and Date: 2014-11-18 – 2014-11-21
• [Presentation] 核酸医薬品の新展開(Significance and applications of novel class of RNA for cabcer therapy) (2014)
  • Author(s): 黒田雅彦
  • Organizer: 第32回日本ヒト細胞学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-08-30 – 2014-08-31
• [Presentation] エクソソームによるドラッグデリバリーシステムの応用(Application of drug delivery systems by exosomes) (2014)
  • Author(s): 黒田雅彦、高梨正勝、大野慎一郎.
  • Organizer: 第6回日本RNAi研究会(JARI)、 第1回日本細胞外小胞学会(JSEV)
  • Place of Presentation: 広島
  • Year and Date: 2014-08-28 – 2014-08-30
• [Presentation] がんを効果的に抑制する短縮型Mimic microRNA の開発. (2014)
  • Author(s): 大野慎一郎、板野華蓮、原田裕一郎、高梨正勝、須藤カツ子、黒田雅彦
  • Organizer: 第33回分子病理学研究会~宮城蔵王シンポジウム~
  • Place of Presentation: 宮城
  • Year and Date: 2014-07-25 – 2014-07-26
• [Presentation] Colon cancer stem cells and microRNA signature during cokon cancer development. (2014)
  • Author(s): Kuroda M.
  • Organizer: 2nd International Symposium 2014.
  • Place of Presentation: Seoul, KOREA
  • Year and Date: 2014-07-07
• [Presentation] Novel RNAi agent can control HCV replication. (2014)
  • Author(s): Murakami Y, Ohno S, Kuroda M
  • Organizer: AACR Annual Meeting 2014
  • Place of Presentation: San Diego, CA
  • Year and Date: 2014-04-05 – 2014-05-09
• [Presentation] 肺がんを効果的に抑制するMimic microRNAの新規形状の探索. (2014)
  • Author(s): 大野 慎一郎、板野 華蓮、原田 裕一郎、高梨 正勝、須藤 カツコ、黒田 雅彦.
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島
• [Presentation] 胃癌細胞株における上皮間葉移行に関与するマイクロRNA200cとその標的の検出. (2014)
  • Author(s): 倉田 厚、山田 正俊、橋本 浩次、藤田 浩司、大野 慎一郎、高梨 正勝、黒田 雅彦.
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島
• [Presentation] 自己由細胞由来のエクソソームによるドラッグデリバリーシステムへの応用 (2014)
  • Author(s): 高梨 正勝、上田 しのぶ、須藤 カツ子、石川 章夫、黒田 雅彦
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島
• [Presentation] 胃癌組織におけるPD-L1の発現検討. (2014)
  • Author(s): 山田 正俊、藤田 浩司、倉田 厚、黒田 雅彦.
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島
• [Presentation] Significance and applications of novel class of RNA for cancer therapy. (2014)
  • Author(s): 黒田 雅彦
  • Organizer: Drug Discovery World Asia
  • Place of Presentation: Singapore
  • Invited
• [Presentation] Novel RNAi agent can control HCV replication. (2014)
  • Author(s): 黒田 雅彦
  • Organizer: RNA Silencing(A9) Keystone symposia.
  • Place of Presentation: Seattle Washington, USA
• [Presentation] 核酸医薬品の開発 (2014)
  • Author(s): 黒田 雅彦
  • Organizer: 日本がん分子標的治療学会
  • Place of Presentation: 東京
  • Invited
• [Presentation] miRNA医薬品の開発 (2013)
  • Author(s): 黒田 雅彦
  • Organizer: 第６回岐阜大学先端創薬医療シンポジウム
  • Place of Presentation: 東京
  • Invited
• [Presentation] 疾患関連miRNAを標的にした臨床医薬品の開発． (2013)
  • Author(s): 黒田 雅彦
  • Organizer: 第40回日本臓器保存生物医学会学術集会
  • Place of Presentation: 東京
  • Invited
• [Presentation] マイクロRNAのがん治療および癌診断への応用 (2013)
  • Author(s): 黒田 雅彦
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Invited
• [Presentation] 核酸医薬品の開発のためのトランスレーショナルリサーチ (2013)
  • Author(s): 黒田 雅彦
  • Organizer: 経バイオテクオンラインプロフェッショナルセミナー
  • Place of Presentation: 東京
  • Invited
• [Presentation] Exosomeによるがん特異的miRNAデリバリー法の開発. (2013)
  • Author(s): 黒田 雅彦、大野慎一郎、高梨 正勝.
  • Organizer: 第29回日本ＤＤＳ学会学術集会
  • Place of Presentation: 京都
  • Invited
• [Book] 膵癌治療up-to-date 2015 (2014)
  • Author(s): 山田正俊、大野慎一郎、黒田雅彦
  • Total Pages: 421
  • Publisher: 医学図書出版株式会社