| Project/Area Number |
24592042
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka Medical College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
UCHIYAMA Kazuhisa 大阪医科大学, 医学部, 教授 (80232867)
HAYASHI Michihiro 大阪医科大学, 医学部, 教授 (90314179)
TANAKA Satoru 大阪医科大学, 医学部, 講師 (50595741)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | siRNA / 癌幹細胞 / 膵癌 / 発癌モデル / in vitto / 膵腺房細胞 / in vitro / 膵発癌モデル |
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| Outline of Final Research Achievements |
The present study's representative is independently developed, in the experimental system that induces the acinar-to-ductal transdifferentiation, but knockdown model using siRNA was a study to demonstrate the trait as a neoplasm, to confirm the acquisition of the trait it is diverted not been able to, the mechanism of anti-cancer agents acquired resistance of cancer stem cells, focused on the differentiation mechanism of the cancer cells. To confirm the expression of surface antigens of cancer stem cells (CD44, such as CD24) using a human pancreatic cancer cell line. After the confirmation of the expression, but was administered to immunodeficient mice for 3-dimensional culture, cancer stem cells that have been transplanted did not lead to growth. In the future, stable growth by changing the conditions continue to expand to allow confirmation.
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