The in vitro research of bacterial infection of prosthetic vascular grafts.
| Project/Area Number |
24592073
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Yuki 東邦大学, 医学部, 助教 (40385729)
FUJII Takeshiro 東邦大学, 医学部, 准教授 (80287531)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 人工血管感染 / 緑膿菌 / 電子顕微鏡 / 人工血管 |
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| Outline of Final Research Achievements |
Bacterial infection from outer surface of prosthetic vascular graft is a subject of our study. The research was done with the models of contaminated vascular graft to investigate the mechanism of bacterial invasion into prosthetic vascular graft from outer surface. Pseudomonas aeruginosa was selected as the model bacterium for its mobility and ability to thrive in physiological saline. Furthermore, a cross section of the vascular grafts were observed with scanning electron microscopy to confirm the bacterial invasion into vascular grafts. Recently, we perform the experiment to measure the amount of gene of protease from bacteria contacted with gelatin-coated Dacron vascular graft by RT-PCR method. Its pilot study is now ongoing.
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Report
(4 results)
Research Products
(4 results)
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[Presentation] The Effect of Rifampicin Bonded Graft for Bacterial Infection2015
Author(s)
Shinnosuke Okuma, Takeshiro Fujii, Hiroshi Masuhara, Tomoyuki Katayanagi, Yoshio Nunoi, Toru Kameda, Kouta Kawada, Yuzo Katayama, Tsukasa Ozawa, Noritsugu Shiono, Yoshinori Watanabe
Organizer
23rd Annual Meeting of the Asian Society for Cardiovascular and Thoracic Surgery
Place of Presentation
Hong Kong(China)
Year and Date
2015-05-12
Related Report
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