| Project/Area Number |
24592099
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SATO Yuichi 北里大学, 医療衛生学部, 教授 (30178793)
JIANG Shi-Xu 北里大学, 医学部, 准教授 (70276153)
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| Research Collaborator |
NAKASHIMA Hiroyasu 北里大学, 医学部, 助教
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 肺腺癌 / 微乳頭構造 / ビメンチン / 免疫組織化学染色 / 二次元電気泳動 / 網羅的タンパク発現解析 / Lung adenocarcinoma / Micropapillary pattern / Immunohistochemistry / Vimentin / micropapillary pattern / タンパク / 免疫染色 / 臨床病理学的因子 / micropapiｌlary pattern / 網羅的解析 / 2次元電気泳動 |
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| Outline of Final Research Achievements |
The factors conferrng the increased malignancy on lung adenocarcinoma with micropapillary component (AC-MPC) remain to be elucidated. 2-dimensional gel electrophoresis, several proteins differentially expressed between AC-MPC and conventional adenocarcinoma (CAC); in particular, vimentin, one of the proteins, was most up-regulated in AC-MPC. Immunohistochemistry revealed that vimentin was expressed in most of MPC. Within the AC-MPC entity, higher vimentin expression was correlated with more frequent vascular invasion and more advanced node metastasis (P<0.05), and multivariate analysis showed that high vimentin expression and worse node statuses were independent indicators of adverse prognosis (P<0.05). In conclusion, vimentin expression is prevalent and markedly up-regulated in MPC, which might reflect the biological essence of poorer differentiation or dedifferentiation of MPC, and this might have a role in the increase of invasiveness and consequent more malignant nature of MPC.
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