The Mechanism of the Cerebral Vasospasm: Mechanistic role for ATP-binding casscette transporter A1-mediated cholesterol metabolism
Project/Area Number |
24592130
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Cerebral neurosurgery
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Research Institution | Yamaguchi University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
SHIRAO Satoshi 山口大学, 大学院医学系研究科, 助教 (70448281)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | cholesterol / cerebral vasospasm / subarachnoid hemorrhage / ABCA1 / コレステロール / 脳血管攣縮 / くも膜下出血 / Rho-kinase / lipid raft / ABCA 1 |
Outline of Final Research Achievements |
Rho-kinase(ROK)-mediated vascular smooth muscle(VSM) contraction plays a pivotal role in cerebral vasospasm(CV). We previously showed that hypercholesterolemia increased sphingosylphosphorylcholine-ROK-mediated VSM contraction. In this study, we show the effect of cholesterol on CV induced by double-hemorrhage rat subarachnoid hemorrhage(SAH) model. SAH-induced basilar artery contraction was markedly reversed by intracisternal infusion with Y27632, a ROK inhibitor. Although, hypercholesterolemia increased VSM cholesterol and SAH-induced CV, depletion of VSM cholesterol by β-CD inhibited SAH-induced CV. These results indicate that cholesterol potentiates SAH-induced CV, but we could not demonstrate the mechanistic role of ATP-binding casscette transporter A1 on CV.
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Report
(4 results)
Research Products
(3 results)
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[Journal Article] A novel trigger for cholesterol-dependent smooth muscle contraction mediated by the sphingosylphosphorylcholine-Rho-kinase pathway in the rat basilar artery: a mechanistic role for lipid rafts2015
Author(s)
Satoshi Shirao, Hiroshi Yoneda, Mizuya Shinoyama, Kazutaka Sugimoto, Hiroyasu Koizumi, Hideyuki Ishihara, Fumiaki Oka, Hirokazu Sadahiro, Sadahiro Nomura, Masami Fujii, Masakatsu Tamechika, Yoshiteru Kagawa, Yuji Owada, and Michiyasu Suzuki
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Journal Title
Journal of Cerebral Blood Flow and Metabolism
Volume: 印刷中
Issue: 5
Pages: 1-8
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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