| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Rho-kinase(ROK)-mediated vascular smooth muscle(VSM) contraction plays a pivotal role in cerebral vasospasm(CV). We previously showed that hypercholesterolemia increased sphingosylphosphorylcholine-ROK-mediated VSM contraction. In this study, we show the effect of cholesterol on CV induced by double-hemorrhage rat subarachnoid hemorrhage(SAH) model. SAH-induced basilar artery contraction was markedly reversed by intracisternal infusion with Y27632, a ROK inhibitor. Although, hypercholesterolemia increased VSM cholesterol and SAH-induced CV, depletion of VSM cholesterol by β-CD inhibited SAH-induced CV. These results indicate that cholesterol potentiates SAH-induced CV, but we could not demonstrate the mechanistic role of ATP-binding casscette transporter A1 on CV.
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