A study of the innovative treatment for rheumatoid arthritis by regulating synoviocytes

• Project/Area Number: 24592261
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kobe University
• Principal Investigator: Miura Yasushi 神戸大学, 保健学研究科, 准教授 (60346244)
• Research Collaborator: FUKUDA Koji ; MAEDA Toshihisa
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 関節リウマチ / 滑膜線維芽細胞 / 滑膜 / インターロイキン12B

Outline of Final Research Achievements

The purpose of this study is to reveal the function of the signaling pathway with decoy receptor 3 (DR3) and its specific ligand TL1A on the synovitis of rheumatoid arthritis (RA) in order to establish a new therapy for RA. We newly found that the set of genes of which expression regulated by DcR3 on RA synovial fibroblasts by using microarray gene analysis. Further, we found that the expression of TPH1 and IL-12B(p40) was regulated by DcR3 and contributed to the pathogenesis of RA. We revealed that DcR3-TL1A signaling on RA synovial fibroblasts is a possible new treatment target of RA.

Research Products

• [Journal Article] Interleukin‑12B is upregulated by decoy receptor 3 in rheumatoid synovial fibroblasts (2016)
  • Author(s): Fukuda K, Miura Y, Maeda T, Hayashi S, Kurosaka M
  • Journal Title: Mol Med Rep Volume: 13 Issue: 4 Pages: 3647-3652
  • DOI: 10.3892/mmr.2016.4985
  • NAID: 120005866875
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Decoy receptor 3 regulates the expression of tryptophan hydroxylase 1 in rheumatoid synovial fibroblasts (2015)
  • Author(s): Maeda T, Miura Y, Fukuda K, Hayashi S, Kurosaka M
  • Journal Title: Mol Med Rep Volume: 12 Issue: 4 Pages: 5191-5196
  • DOI: 10.3892/mmr.2015.4097
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Decoy receptor 3 regulates the expression of tryptophan hydroxylase 1 in rheumatoid synovial fibroblasts (2015)
  • Author(s): Toshihisa Maeda, Yasushi Miura, Koji Fukuda, Shinya Hayashi, Masahiro Kurosaka
  • Journal Title: Molecular Medicine Reports Volume: TBA
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] DcR3による遺伝子発現誘導 最新関節リウマチ学-寛解・治癒を目指した研究と最新治療- 関節リウマチの発症要因と発症メカニズム (2014)
  • Author(s): 林申也、三浦靖史、黒坂昌弘
  • Journal Title: 日本臨牀 Volume: 72S3 Pages: 68-72
• [Journal Article] Decoy receptor 3 regulates the expression of various genes in rheumatoid arthritis synovial fibroblasts (2013)
  • Author(s): Fukuda K, Miura Y, Maeda T, Takahashi M, Hayashi S, Kurosaka K.
  • Journal Title: Int J Mol Med Volume: 32 Issue: 4 Pages: 910-916
  • DOI: 10.3892/ijmm.2013.1461
• [Presentation] Expression of TNF-like ligand 1A (TL1A) in rheumatoid synovial fibroblasts Maeda T, Miura Y, Fukuda K, Hayashi S, Kurosaka M (2016)
  • Author(s): The 62th Annual Meeting of the Orthopaedic Research Society
  • Organizer: The 62th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: オーランド、アメリカ
  • Year and Date: 2016-03-05
• [Presentation] TL1Aによるリウマチ滑膜線維芽細胞における遺伝子発現誘導の検討 (2015)
  • Author(s): 前田俊恒、三浦靖史、福田康治、林申也、黒坂昌弘
  • Organizer: 第30回日本整形外科学会基礎学術集会
  • Place of Presentation: 富山県
  • Year and Date: 2015-10-22
• [Presentation] Centrosomal protein 70kDa is down-regulated by decoy receptor 3 in specifically rheumatoid synovial fibroblasts (2015)
  • Author(s): Fukuda K, Miura Y, Maeda T, Hayashi S, Kurosaka M
  • Organizer: The 16th The European League Against Rheumatism (EULAR 2015)
  • Place of Presentation: ローマ、イタリア
  • Year and Date: 2015-06-10
  • Int'l Joint Research
• [Presentation] Analysis for the expression of TNF-like ligand 1A (TL1A) in rheumatoid synovial fibroblasts (2015)
  • Author(s): Maeda T, Miura Y, Fukuda K, Hayashi S, Kurosaka M
  • Organizer: The 16th The European League Against Rheumatism (EULAR 2015)
  • Place of Presentation: ローマ、イタリア
  • Year and Date: 2015-06-10
  • Int'l Joint Research
• [Presentation] Centrosomal protein 70kDaはdecoy receptor 3により関節リウマチ滑膜細胞に特異的に発現が抑制される (2015)
  • Author(s): 福田康治、三浦靖史、前田俊恒、林申也、黒坂昌弘
  • Organizer: 第59回日本リウマチ学会総会・学術集会
  • Place of Presentation: 愛知県
  • Year and Date: 2015-04-23
• [Presentation] TL1Aによるリウマチ滑膜線維芽細胞における遺伝子発現誘導の検討 (2015)
  • Author(s): 前田俊恒、三浦靖史、福田康治、林申也、黒坂昌弘
  • Organizer: 第59回日本リウマチ学会総会・学術集会
  • Place of Presentation: 愛知県
  • Year and Date: 2015-04-23
• [Presentation] DcR3は関節リウマチ滑膜線維芽細胞におけるトリプトファン水酸化酵素TPH1の発現を制御する (2015)
  • Author(s): 前田俊恒、三浦靖史、福田康治、林申也、黒坂昌弘
  • Organizer: 第124回中部日本整形外科災害外科学会
  • Place of Presentation: 石川県
  • Year and Date: 2015-04-10
• [Presentation] Centrosomal protein 70kDa is down-regulated by decoy receptor 3 in specifically rheumatoid synovial fibroblasts (2015)
  • Author(s): Koji Fukuda, Yasushi Miura, Toshihisa Maeda, Shinya Hayashi, Masahiro Kurosaka
  • Organizer: The 61th Annual Meeting of the Orthopaedic Research Society
  • Place of Presentation: Las Vegas, USA
  • Year and Date: 2015-03-28 – 2015-03-31
• [Presentation] Centrosomal protein 70kDa is down-regulated by decoy receptor 3 in specifically rheumatoid synovial fibroblasts (2014)
  • Author(s): Koji Fukuda, Yasushi Miura, Toshihisa Maeda, Shinya Hayashi, Masahiro Kurosaka
  • Organizer: The 78th AMERICAN COLLEGE OF RHEUMATOLOGY Annual Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2014-11-14 – 2014-11-19
• [Presentation] Interleukin-12B is up-regulated by decoy receptor 3 specifically in rheumatoid synovial fibroblasts (2014)
  • Author(s): Koji Fukuda, Yasushi Miura, Toshihisa Maeda, Shinya Hayashi, Masahiro Kurosaka
  • Organizer: The 15th EULAR Annual European Congress of Rheumatology
  • Place of Presentation: Paris, France
  • Year and Date: 2014-06-11 – 2014-06-14
• [Presentation] Decoy receptor 3 regulates the expression of tryptophan hydroxylase TPH1 in rheumatoid synovial fibroblasts (2014)
  • Author(s): Toshihisa Maeda, Yasushi Miura, Koji Fukuda, Shinya Hayashi, Masahiro Kurosaka
  • Organizer: The 15th EULAR Annual European Congress of Rheumatology
  • Place of Presentation: Paris, France
  • Year and Date: 2014-06-11 – 2014-06-14
• [Presentation] DcR3は関節リウマチ滑膜線維芽細胞におけるトリプトファン水酸化酵素TPH1の発現を制御する (2014)
  • Author(s): 前田俊恒、三浦靖史、福田康治、林申也、黒坂昌弘
  • Organizer: 第58回日本リウマチ学会総会学術集会
  • Place of Presentation: グランドプリンスホテル新高輪（東京都港区）
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] IL-12Bはdecoy receptor 3により関節リウマチ滑膜細胞に特異的に発現誘導される (2014)
  • Author(s): 福田康治、三浦靖史、前田俊恒、林申也、黒坂昌弘
  • Organizer: 第58回日本リウマチ学会総会学術集会
  • Place of Presentation: グランドプリンスホテル新高輪（東京都港区）
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] Decoy receptor 3 regulates the expression of tryptophan hydroxylase TPH1 in rheumatoid synovial fibroblasts (2014)
  • Author(s): Toshihisa Maeda, Yasushi Miura, Koji Fukuda, Shinya Hayashi, Masahiro Kurosaka
  • Organizer: The 16th Asia Pacific League of Associations for Rheumatology Congress APLAR 2014
  • Place of Presentation: Cebu City, Philippines
  • Year and Date: 2014-04-01 – 2014-04-05
• [Presentation] 関節リウマチ滑膜細胞におけるDcR3によるIL-12B p40発現誘導の検討 (2013)
  • Author(s): 福田康治、三浦靖史、前田俊恒、林申也、黒坂昌弘
  • Organizer: 第57回日本リウマチ学会総会・学術集会
  • Place of Presentation: 京都国際会議場
• [Presentation] Decoy receptor 3 regulates the expression of tryptophan hydroxylase TPH1 in rheumatoid synovial fibroblastsを制御する (2013)
  • Author(s): Toshihisa Maeda, Yasushi Miura, Koji Fukuda, Shinya Hayashi, Masahiro Kurosaka
  • Organizer: 14th Annual European Congress of Rheumatology (EULAR)
  • Place of Presentation: Madrid, Spain
• [Presentation] DcR3による関節リウマチ滑膜細胞におけるIL-12B p40発現誘導の検討 (2013)
  • Author(s): 福田康治、三浦靖史、前田俊恒、林申也、黒坂昌弘
  • Organizer: 第28回 日本整形外科学会基礎学術集会
  • Place of Presentation: 幕張メッセ
• [Presentation] Interleukin-12B is up-regulated by decoy receptor 3 in specifically rheumatoid synovial fibroblasts (2013)
  • Author(s): Fukuda K, Miura Y, Maeda T, Hayashi S, Kurosaka M
  • Organizer: 77th ACR/ARHP Annual Scientific Meeting
  • Place of Presentation: San Diego
• [Presentation] Decoy receptor 3 increases the expression of IL-12B in rheumatoid synovial fibroblasts (2013)
  • Author(s): Fukuda K, Miura Y, Maeda T, Hayashi S, Kurosaka M
  • Organizer: 15th Asia Pacific League of Associations for Rheumatology Congress (APLAR)
  • Place of Presentation: Bali, Indonesia
• [Presentation] 関節リウマチ滑膜細胞におけるDcR3による遺伝子発現誘導の検討 (2012)
  • Author(s): 福田康治、三浦靖史、前田俊恒、林申也、高橋完靖、黒坂昌弘
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋
• [Presentation] 関節リウマチ滑膜線維芽細胞におけるトリプトファン水酸化酵素TPH1発現の検討 (2012)
  • Author(s): 前田俊恒、三浦靖史、福田康治、林申也、黒坂昌弘
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋
• [Presentation] Decoy receptor 3 influences the gene expression of various key molecules in rheumatoid synovial fibroblasts (2012)
  • Author(s): Koji Fukuda, Yasushi Miura, Toshihisa Maeda, Shinya Hayashi, Masayasu Takahashi, Masahiro Kurosaka
  • Organizer: EULAR2012
  • Place of Presentation: Berline
• [Remarks] 三浦研究室ホームページ
  • URL: http://www2.kobe-u.ac.jp/~ymiura/hp/home.html
• [Remarks] 三浦ホームページ
  • URL: http://www2.kobe-u.ac.jp/~ymiura/hp/home.html