| Project/Area Number |
24592271
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
CHOSA Etsuo 宮崎大学, 医学部, 教授 (00236837)
SEKIMOTO Tomohisa 宮崎大学, 医学部, 講師 (60305000)
ARAKI Masatake 熊本大学, 生命資源研究・支援センター, 准教授 (80271609)
ARAKI Kimi 熊本大学, 生命資源研究・支援センター, 教授 (90211705)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | Lima1 / EPLIN / 骨代謝 / 骨芽細胞 / EGTC / モデルマウス |
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| Outline of Final Research Achievements |
We analyzed the bone phenotype of Lima1/EPLIN trapped mice that were produced by the exchageable gene trap method. We underwent micro-CT imaging, bone morphometry analysis, bone strength testing, various staining of bone tissue specimens, and realtime PCR analysis. Bone strength and bone density of the femur had decreased in these mice. In the alkaline phosphatase staining of bone tissue specimens, osteoblasts of the gene trapped mice had been reduced its activity. And the signal of type I collagen had been also decreased in comparison with wild type mice in in situ hybridization. Furthermore, realtime PCR revealed that expression levels of the various genes associated to bone formation such as BMP2, Col1a1, and osteocalcin were decreased in the gene trapped mice. From the results of these analysis, it was suggested that Lima1/EPLIN is involved in the differentiation and/or function of osteoblasts.
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