| Project/Area Number |
24592286
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
YUI NAOKO 聖マリアンナ医科大学, 医学部, 助教 (20266696)
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| Co-Investigator(Kenkyū-buntansha) |
YUDOH Kazuo 聖マリアンナ医科大学, 医学研究科, 教授 (60272928)
KARASAWA Rie 聖マリアンナ医科大学, 医学研究科, 講師 (50424410)
唐澤 里江 聖マリアンナ医科大学, 医学(系)研究科(研究院), 講師 (50434410)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 変形性関節症 / 軟骨細胞 / ＤＮＡ修復酵素 / 酸化ストレス / DNA修復酵素 / 変性 / ＡＰＥＸ２ |
|---|
| Outline of Final Research Achievements |
Apex 2 plays a critical role in DNA repair caused by oxidative damage in a variety of human somatic cells. We speculated that chondrocyte Apex 2 may protect against the catabolic process of articular cartilage in osteoarthritis (OA). Higher levels of Apex 2 expression were histologically observed in severely compared with mildly degenerated OA cartilage from STR/OrtCrlj mice, The immunopositivity of Apex 2 was significantly correlated with the degree of cartilage degeneration. Moreover, interleukin-1β induced the expression of Apex 2 in chondrocytes, while Apex 2 silencing using small interfering RNA reduced chondrocyte activity in vitro. Our findings suggest that Apex 2 may have the potential to prevent the catabolic stress-mediated down-regulation of chondrocyte activity in OA.
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