Analysis of orexin-receptor functions

• Project/Area Number: 24592306
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: University of Miyazaki
• Principal Investigator: SHIRASAKA Tetsuro 宮崎大学, 医学部, 准教授 (00274788)
• Co-Investigator(Kenkyū-buntansha): YANO Takeshi 宮崎大学, 医学部・集中治療部, 助教 (80521707)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
• Keywords: オレキシン / 麻酔 / 睡眠と覚醒 / モノアミン / グルタミン / オレキシン受容体

Outline of Final Research Achievements

Intracerebroventricular (i.c.v.) injection of orexin A(OXA) produces an increase in blood pressure and heart rate. The cardiovascular responses induced by i.c.v.-injection of OXA were mainly mediated by OX1-receptor rather than OX2-receptor. I.c.v.-injection of OXA increased NE and Glu mediated by OX1-receptor and OX2-receptor. These responses were inhibited by propofol and sevoflurane in a concentration dependent manner, but not by dexmedetomidine and ketamine, These results suggests that OXA increase NE and Glu in the prefrontal cortex mediated by GABAA receptor.