Functional analysis of magnetic nanoparticles and the related gene for prostate cancer therapy

• Project/Area Number: 24592379
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Yokohama National University
• Principal Investigator: WATANABE Masatoshi 横浜国立大学, 工学(系)研究科(研究院), 教授 (90273383)
• Co-Investigator(Kenkyū-buntansha): SHIRAISHI Taizo 三重大学, 医学(系)研究科(研究院), 教授 (30162762)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 前立腺癌 / ナノ粒子 / 抗癌剤 / 癌 / ナノ材料

Outline of Final Research Achievements

Magnetite nanoparticles (MgNPs), have a unique property of superparamagnetism and reactive oxygen specimens (ROS) production, which can enhance the delivery and treatment efficiency of anti-cancer drugs. In this study, we have shown that in vitro enhanced effects of bare/surface modified MgNPs combined with docetaxel in comparison with docetaxel in prostate cancer cells via different mechanisms. In addition, effect of MgNPs on zyxin expression may be related with ROS production.

Research Products

• [Journal Article] 前立腺癌治療へのナノ粒子の応用. (2015)
  • Author(s): 岩﨑有由美、岡本大樹、遠藤宣弘、渡邉昌俊.
  • Journal Title: 医学のあゆみ Volume: 252 Pages: 303-308
  • Acknowledgement Compliant
• [Journal Article] Magnetite Nanoparticles Induce Genotoxicity in the Lungs of Mice via Inflammatory Response (2014)
  • Author(s): Totsuka Y, Ishino K, Kato T, Goto S, Tada Y, Nakae D, Watanabe M, Wakabayashi K
  • Journal Title: Nanomaterials Volume: 4 Issue: 1 Pages: 175-188
  • DOI: 10.3390/nano4010175
  • Peer Reviewed / Open Access
• [Journal Article] Pleiotropic functions of magnetic nanoparticles for ex vivo gene transfer. (2014)
  • Author(s): Kami D, Kitani T, Kishida T, Mazda O, Toyoda M, Tomitaka A, Ota S, Ishii R, Takemura Y, Watanabe M, Umezawa A, Gojo S.
  • Journal Title: Nanomedicine Volume: 10 Issue: 6 Pages: 1165-1174
  • DOI: 10.1016/j.nano.2014.03.018
  • Peer Reviewed / Open Access
• [Journal Article] Effects of Fe3O4 Magnetic Nanoparticles on A549 Cells. (2013)
  • Author(s): (95) M.Watanabe, M.Yoneda, A.Morohashi, Y.Hori, D.Okamoto, A.Sato, D.Kurioka, T.Nittami, H.Hirokawa, T.Shiraishi, K.Kawai, H.Kasai and Y.Totsuka.
  • Journal Title: Int.J.Mol.Sci. Volume: 14 Issue: 8 Pages: 15546-15560
  • DOI: 10.3390/ijms140815546
  • Peer Reviewed
• [Journal Article] Transfection efficiency influenced by aggregation of DNA/polyethylenimine max/magnetic nanoparticle complexes. (2013)
  • Author(s): (96) S.Ota, Y.Takahashi, A.Tomitaka, T.Yamada, D.Kami, M.Watanabe, Y.Takemura.
  • Journal Title: J Nanopart Res Volume: 15 Pages: 1653-1664
  • Peer Reviewed
• [Journal Article] Magnetic nanoparticles of Fe3O4 enhance docetaxel-induced prostate cancer cell death. (2013)
  • Author(s): A.Sato, N.Itcho, H.Ishiguro, D.Okamoto, N.Kobayashi, K.Kawai, H.Kasai, D.Kurioka, H.Uemura, Y.Kubota, M.Watanabe.
  • Journal Title: Int J Nanomed Volume: 8
  • Peer Reviewed
• [Journal Article] 前立腺がん化学療法における磁性体ナノ粒子の効果. (2013)
  • Author(s): 栗岡大輔，佐藤明子，岩崎有由美，深井瑛美，岡本大樹，渡邉昌俊.
  • Journal Title: 化学工業 Volume: 64 Pages: 67-73
  • NAID: 10031135730
• [Journal Article] Genotoxicity of multi-welled carbon nanotubes in both in vitro and in vivo assay systems. (2012)
  • Author(s): T.Kato, Y.Totsuka, K.Ishino, Y.Matsumoto, Y.Toda, D.Nakae, S.Goto, S.Masuda, S.Ogo, M.Kawanishi, T.Yagi, T.Matsuda, M.Watanabe, K.Wakabayashi.
  • Journal Title: Nanotoxicology Volume: 7 Pages: 452-461
  • Peer Reviewed
• [Presentation] 前立腺癌細胞におけるカルボキシル基修飾磁性体ナノ粒子とドセタキセルの併用効果とその機構について (2014)
  • Author(s): 岡本大樹、古田奈緒、岩﨑有由美、山口創、栗岡大輔、新田見匡、河井一明、葛西宏、石黒斉、窪田吉信、上村博司、渡邉昌俊
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 前立腺癌細胞における磁性体ナノ粒子と各種化学療法剤の併用効果(II) (2014)
  • Author(s): 岩﨑有由美、橋本紗歩、山口創、古田奈緒、岡本大樹、深井瑛美、栗岡大輔、新田見匡、河井一明、葛西宏、渡邉昌俊
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 前立腺癌細胞における磁性体ナノ粒子とドセタキセルの併用効果とその機構について (2014)
  • Author(s): 古田奈緒、岩﨑有由美、山口創、岡本大樹、栗岡大輔、新田見匡、河井一明、葛西宏、石黒斉、窪田吉信、上村博司、渡邉昌俊
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 磁性体ナノ粒子の前立腺癌におけるドキソルビシンによる治療への効果 (2014)
  • Author(s): 山口創、橋本紗歩、古田奈緒、岩﨑有由美、岡本大樹、深井榮美、栗岡大輔、新田見匡、渡邉昌俊
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Nanomedicine in prostate acncer therapy. (2014)
  • Author(s): M.Watanabe, Y.Endo, D.Kurioka, A.Sato, A.Iwasaki, D.Okamoto, N.Watanabe
  • Organizer: 101st Indian Science Congress
  • Place of Presentation: Jammu, India
  • Invited
• [Presentation] Synergistic effect of carboxyl-modified magnestic nanoparticles and docetaxel on prostate cancer cells in vitro. (2013)
  • Author(s): M.Watanabe, A.Sato, D.Okamoto, D.Kurioka, H.Ishiguro, H.Uemura, Y.Kubota.
  • Organizer: Annual Meeting 2013, AACR
  • Place of Presentation: Washington, DC
• [Presentation] 高濃度曝露の磁性体ナノ粒子はA549細胞における抗酸化システムを阻害し，細胞毒性性をもたらす。 (2013)
  • Author(s): 古田奈緒、山口創、岩﨑有由美、岡本大樹、佐藤明子、河井一明、葛西宏、戸塚ゆ加里、渡邉昌俊
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜コンベンションセンター
• [Presentation] 前立腺癌細胞における磁性体ナノ粒子と各種化学療法剤の併用効果 (2013)
  • Author(s): 岩﨑有由美、古田奈緒、山口創、岡本大樹、深井瑛美、佐藤明子、河井一明、葛西宏、石黒斉、上村博司、窪田吉信、渡邉昌俊
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜コンベンションセンター
• [Presentation] 前立腺癌細胞におけるカルボキシル基修飾磁性体ナノ粒子とドセタキセルの併用効果(II) (2013)
  • Author(s): 岡本大樹、古田奈緒、山口創、岩﨑有由美、佐藤明子、河井一明、葛西宏、渡邉昌俊
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜コンベンションセンター
• [Presentation] Synergistic effect of magnetic nanoparticles and docetaxel on prostate cancer cells in vitro. (2012)
  • Author(s): A. Sato, D. Kurioka, H. Ishiguro, H. Uemura, Y. Kubota, M. Watanabe.
  • Organizer: Annual Meeting 2012, AACR
  • Place of Presentation: Chicago, IL, USA
• [Presentation] 磁性体ナノ粒子は前立腺癌に対するドセタキセルの効果を増強する. (2012)
  • Author(s): 佐藤明子，諸橋彩香，岩崎有由美，石黒斉，植村博司，窪田吉信，渡辺昌俊.
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] 前立腺癌におけるカルボキシル基就職磁性体ナノ粒子とドセタキセルの併用効果. (2012)
  • Author(s): 岡本大樹，深井瑛美，岩﨑有由美，佐藤明子，白石泰三，河井一明，葛西宏，石黒斉，渡辺昌俊.
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] 磁性体ナノ粒子が曝露したがん細胞における抗酸化酵素遺伝子および活性酸素種酸性の変化について. (2012)
  • Author(s): 諸橋彩香，佐藤明子，岩﨑有由美，河井一明，葛西宏，石黒斉，古林直人，渡辺昌俊.
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌