Identification of mechanism of bladder cancer development with Ral associated molecular network

Research Project

Project/Area Number	24592386
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Urology
Research Institution	Kyoto University
Principal Investigator	MATSUI Yoshiyuki 京都大学, 医学(系)研究科(研究院), 講師 (00582107)
Co-Investigator(Kenkyū-buntansha)	OGAWA Osamu 京都大学, 医学研究科, 教授 (90260611) 大久保和俊京都大学, 医学(系)研究科(研究院), 講師 (20511877) 今村正明京都大学, 医学(系)研究科(研究院), 助教 (20594237)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000) Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords	膀胱癌
Outline of Final Research Achievements	We recognized that the activation of Ral induced by the down-regulation of RalGAPα2 had a possibility to be related to the bladder carcinogenesis, through suppressing Filamin A expression. We confirmed that the immunostaining intensity of Filamin A in human bladder cancer tissue was correlated with pathological aggressiveness and infiltration. Suppression of Filamin A led to reduced proliferation in bladder cancer cell lines, whereas we did not observe significant changes in cell migration and invasion. Filamin A did not affect the tumor growth and local invasion ability in subcutaneous and renal subcapsular xenograft models.