| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Outline of Final Research Achievements |
Papillary cell adhesion molecule (pCAM) is a adhesion molecule of cell-cell and cell-basal lamina and expressing in only neuroendocrine tissue. We examined whether the pCAM was a key molecule of cell differentiation and pathological architecture in papillary renal cell carcinoma (RCC). A human RCC cell line, 786-O highly expressing pCAM was undifferentiated clear cell RCC. Downregulation of the pCAM expression made the 786-O tumor to be well differentiated papillary RCC in the kidney of athymic nude mouse. Re-expression of pCAM by site-directed mutagenesis method returned 786-O tumor to be undifferentiated clear cell RCC. We demonstrated the architectural transformation accompanied with changing epithelial mesenchymal transition (EMT) factors by PCR array 786O. In conclusions, the pCAM is a key molecule for papillary formation of RCC.
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