The effect of Nitric Oxide (NO) treatment on renal cell cancer

• Project/Area Number: 24592404
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: HONGO Fumiya 京都府立医科大学, 医学(系)研究科(研究院), 助教 (80291798)
• Co-Investigator(Kenkyū-buntansha): MIKI Tsuneharu 京都府立医科大学, 医学研究科, 教授 (10243239) ; KAWAUCHI Akihiro 滋賀医科大学, 医学部, 教授 (90240952) ; NAKAMURA Terukazu 京都府立医科大学, 医学研究科, 講師 (10381964) ; KIMURA Yasunori 京都府立医科大学, 医学研究科, 助教 (20398374)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 腎癌 / 一酸化窒素 / sニトロソ化

Outline of Final Research Achievements

Purpose and methods: Nitric oxide (NO) is a signaling molecule. This post- translational modification by NO is known as S-nitrosylation. We examined the effect of the NO donor DETA-NONOate on the renal cell cancer cell lines; CAKI-1, NC65, and ACHN. Anti-S-nitroso-Cysteine antibody was applied as a primary antibody for immunohistochemistry. The biotin-switch technique was applied to detect specific S-nitrosylated proteins.
Results and conclusions: Significant up-regulation of S-nitrosylated proteins employed with NO donor was observed by immunohistochemistry. Specific S-nitrosylated proteins in ACHN cells were detected by the biotin-switch technique and included HSP90 (heat shock protein 90), GRP78 (HSP70 family), HSP70, and pyruvate kinase M2. In ACNH cells, Significant up regulation of S-nitrosylated HSP90 by western blot. Conclusions: Our data showed that NO treatment significantly increased S-nitrosylation of several proteins.

Research Products

• [Presentation] Detection of S-nitrosylated heat shock protein 90 (HSP90) in renal cell cancer. (2014)
  • Author(s): Hongo F, Ueda T, Ito-Ueda S, Oishi M, Nakamura T, Naya Y, Miki T.
  • Organizer: AACR Annual Meeting 2014
  • Place of Presentation: San Diego, USA.
  • Year and Date: 2014-05-05 – 2014-05-09
• [Presentation] Detection of S-nitrosylated heat shock protein 90 (HSP90) in renal cell cancer. (2014)
  • Author(s): Fumiya Hongo, Takashi Ueda, Saya Ito-Ueda, Masakatsu Oishi, Terukazu Nakamura, Yoshio Naya, Tsuneharu Miki
  • Organizer: American Association for Cancer Research 2014
  • Place of Presentation: San Diego, USA.
• [Presentation] Detection of S-nitrosylated protein in renal cell cancer (2013)
  • Author(s): Fumiya Hongo, Takashi Ueda, Saya Ito-Ueda, Masakatsu Oishi, Terukazu Nakamura, Yoshio Naya, Tsuneharu Miki
  • Organizer: American Association for Cancer Research 2013
  • Place of Presentation: Washington DC, USA.
• [Presentation] Detection of S-nitrosylated protein in renal cell cancer (2013)
  • Author(s): Fumiya Hongo, Takashi Ueda, Saya Ito-Ueda, Masakatsu Oishi, Terukazu Nakamura, Yoshio Naya, Tsuneharu Miki
  • Organizer: Ａｍｅｒｉｃａｎ Ａｓｓｏｓｉａｔｅ Ｃａｎｃｅｒ Ｒｅｓｅｒｃｈ
  • Place of Presentation: Washington DC, USA
• [Presentation] 腎癌におけるS-ニトロソ化タンパクの同定
  • Author(s): 本郷文弥、上田崇、伊藤紗弥、大石正勝、中村晃和、納谷佳男、三木恒治
  • Organizer: 第101回日本泌尿器科学会総会
  • Place of Presentation: 札幌
• [Presentation] Detection of protein S-nitrosylation by the biotin-switch technique in renal cell cancer cells
  • Author(s): Fumiya Hongo, Takashi Ueda, Saya Ito-Ueda, Yasunori Kimura, Terukazu Nakamura, Yoshio Naya, , Tsuneharu Miki
  • Organizer: 第71回日本癌学会総会
  • Place of Presentation: 札幌