| Project/Area Number |
24592404
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
HONGO Fumiya 京都府立医科大学, 医学(系)研究科(研究院), 助教 (80291798)
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| Co-Investigator(Kenkyū-buntansha) |
MIKI Tsuneharu 京都府立医科大学, 医学研究科, 教授 (10243239)
KAWAUCHI Akihiro 滋賀医科大学, 医学部, 教授 (90240952)
NAKAMURA Terukazu 京都府立医科大学, 医学研究科, 講師 (10381964)
KIMURA Yasunori 京都府立医科大学, 医学研究科, 助教 (20398374)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 腎癌 / 一酸化窒素 / sニトロソ化 |
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| Outline of Final Research Achievements |
Purpose and methods: Nitric oxide (NO) is a signaling molecule. This post- translational modification by NO is known as S-nitrosylation. We examined the effect of the NO donor DETA-NONOate on the renal cell cancer cell lines; CAKI-1, NC65, and ACHN. Anti-S-nitroso-Cysteine antibody was applied as a primary antibody for immunohistochemistry. The biotin-switch technique was applied to detect specific S-nitrosylated proteins.
Results and conclusions: Significant up-regulation of S-nitrosylated proteins employed with NO donor was observed by immunohistochemistry. Specific S-nitrosylated proteins in ACHN cells were detected by the biotin-switch technique and included HSP90 (heat shock protein 90), GRP78 (HSP70 family), HSP70, and pyruvate kinase M2. In ACNH cells, Significant up regulation of S-nitrosylated HSP90 by western blot. Conclusions: Our data showed that NO treatment significantly increased S-nitrosylation of several proteins.
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