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The effect of Nitric Oxide (NO) treatment on renal cell cancer

Research Project

Project/Area Number 24592404
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Urology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

HONGO Fumiya  京都府立医科大学, 医学(系)研究科(研究院), 助教 (80291798)

Co-Investigator(Kenkyū-buntansha) MIKI Tsuneharu  京都府立医科大学, 医学研究科, 教授 (10243239)
KAWAUCHI Akihiro  滋賀医科大学, 医学部, 教授 (90240952)
NAKAMURA Terukazu  京都府立医科大学, 医学研究科, 講師 (10381964)
KIMURA Yasunori  京都府立医科大学, 医学研究科, 助教 (20398374)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords腎癌 / 一酸化窒素 / sニトロソ化
Outline of Final Research Achievements

Purpose and methods: Nitric oxide (NO) is a signaling molecule. This post- translational modification by NO is known as S-nitrosylation. We examined the effect of the NO donor DETA-NONOate on the renal cell cancer cell lines; CAKI-1, NC65, and ACHN. Anti-S-nitroso-Cysteine antibody was applied as a primary antibody for immunohistochemistry. The biotin-switch technique was applied to detect specific S-nitrosylated proteins.
Results and conclusions: Significant up-regulation of S-nitrosylated proteins employed with NO donor was observed by immunohistochemistry. Specific S-nitrosylated proteins in ACHN cells were detected by the biotin-switch technique and included HSP90 (heat shock protein 90), GRP78 (HSP70 family), HSP70, and pyruvate kinase M2. In ACNH cells, Significant up regulation of S-nitrosylated HSP90 by western blot. Conclusions: Our data showed that NO treatment significantly increased S-nitrosylation of several proteins.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (6 results)

All 2014 2013 Other

All Presentation (6 results)

  • [Presentation] Detection of S-nitrosylated heat shock protein 90 (HSP90) in renal cell cancer.2014

    • Author(s)
      Hongo F, Ueda T, Ito-Ueda S, Oishi M, Nakamura T, Naya Y, Miki T.
    • Organizer
      AACR Annual Meeting 2014
    • Place of Presentation
      San Diego, USA.
    • Year and Date
      2014-05-05 – 2014-05-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] Detection of S-nitrosylated heat shock protein 90 (HSP90) in renal cell cancer.2014

    • Author(s)
      Fumiya Hongo, Takashi Ueda, Saya Ito-Ueda, Masakatsu Oishi, Terukazu Nakamura, Yoshio Naya, Tsuneharu Miki
    • Organizer
      American Association for Cancer Research 2014
    • Place of Presentation
      San Diego, USA.
    • Related Report
      2013 Research-status Report
  • [Presentation] Detection of S-nitrosylated protein in renal cell cancer2013

    • Author(s)
      Fumiya Hongo, Takashi Ueda, Saya Ito-Ueda, Masakatsu Oishi, Terukazu Nakamura, Yoshio Naya, Tsuneharu Miki
    • Organizer
      American Association for Cancer Research 2013
    • Place of Presentation
      Washington DC, USA.
    • Related Report
      2013 Research-status Report
  • [Presentation] Detection of S-nitrosylated protein in renal cell cancer2013

    • Author(s)
      Fumiya Hongo, Takashi Ueda, Saya Ito-Ueda, Masakatsu Oishi, Terukazu Nakamura, Yoshio Naya, Tsuneharu Miki
    • Organizer
      American Assosiate Cancer Reserch
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] 腎癌におけるS-ニトロソ化タンパクの同定

    • Author(s)
      本郷文弥、上田崇、伊藤紗弥、大石正勝、中村晃和、納谷佳男、三木恒治
    • Organizer
      第101回日本泌尿器科学会総会
    • Place of Presentation
      札幌
    • Related Report
      2013 Research-status Report 2012 Research-status Report
  • [Presentation] Detection of protein S-nitrosylation by the biotin-switch technique in renal cell cancer cells

    • Author(s)
      Fumiya Hongo, Takashi Ueda, Saya Ito-Ueda, Yasunori Kimura, Terukazu Nakamura, Yoshio Naya, , Tsuneharu Miki
    • Organizer
      第71回日本癌学会総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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