Study for a new measurement for the mammalian sperm oocyte-activation ability
| Project/Area Number |
24592441
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Chiba University |
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Principal Investigator |
ITO Chizuru 千葉大学, 医学(系)研究科(研究院), 講師 (80347054)
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| Co-Investigator(Kenkyū-buntansha) |
TOSHIMORI Kiyotaka 千葉大学, 大学院医学研究院, 教授 (20094097)
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| Co-Investigator(Renkei-kenkyūsha) |
MAEKAWA Mamiko 千葉大学, 大学院医学研究院, 助教 (20181571)
YAMATOYA Kenji 千葉大学, 大学院医学研究院, 日本学術振興会特別研究員(PD) (80447309)
KAMIMURA Kyouko 千葉大学, 大学院医学研究院, 技術専門職員 (20422264)
MUTOH Tohru 千葉大学, 大学院医学研究院, 技術専門職員 (30422265)
TOYAMA Yoshirou 千葉大学, 大学院医学研究院, 講師 (70009637)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 精子 / 核周囲物質 / 中心体関連タンパク質 / 卵活性化 / Equatorin / ODF2 / MN13 / 不妊症 / 卵活性化能 / equatorin |
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| Outline of Final Research Achievements |
For the assessment of sperm oocyte-activation ability, we analyzed an acrosomal and perinuclear theca (PT)-related protein Equatorin and a centrosome scaffold component ODF2 using transgenic mice. We clarified the expression, localization and translocation route of Equatorin and ODF2c during the spermatogenesis and the fate of them in the fertilization process. We also showed that the distribution of Equatorin and PT substance MN13 in the sperm reflected the sperm fertilization ability by comparing sperm of infertile patients to those of fertile volunteers having children.
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Report
(4 results)
Research Products
(55 results)
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[Journal Article] A mutation of COX6A1 causes a recessive axonal or mixed form of Charcot-Marie-Tooth disease.2014
Author(s)
Tamiya G, Makino S, Hayashi M, Abe A, Numakura C, Ueki M, Tanaka A, Ito C, Toshimori K, Ogawa N, Terashima T, Maegawa H, Yanagisawa D, Tooyama I, Tada M, Onodera O, Hayasaka K.
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Journal Title
Am J Hum Genet.
Volume: 95
Issue: 3
Pages: 294-300
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A heterozygous mutation of GALNTL5 affects male infertility with impairment of sperm motility2014
Author(s)
Takasaki N, Tachibana K, Ogasawara S, Matsuzaki H, Hagiuda J, Ishikawa H, Mochida K, Inoue K, Ogonuki N, Ogura A, Noce T, Ito C, Toshimori K, Narimatsu H.
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Journal Title
Proc Natl Acad Sci USA
Volume: 111
Issue: 3
Pages: 1120-1125
DOI
Related Report
Peer Reviewed
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[Journal Article] Oligo-astheno-teratozoospermia in mice lacking ORP4, a sterol-binding protein in the OSBP-related protein family.2014
Author(s)
Udagawa O, Ito C, Ogonuki N, Sato H, Lee S, Tripvanuntakul P, Ichi I, Uchida Y, Nishimura T, Murakami M, Ogura A, Inoue T, Toshimori K, Arai H.
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Journal Title
Genes Cells.
Volume: 19
Issue: 1
Pages: 13-27
DOI
Related Report
Peer Reviewed
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[Journal Article] The Arf GAP SMAP2 is necessary for organized vesicle budding from the trans-Golgi network and subsequent acrosome formation in spermiogenesis.2013
Author(s)
Funaki T, Kon S, Tanabe K, Natsume W, Sato S, Shimizu T, Yoshida N, Wong WF, Ogura A, Ogawa T, Inoue K, Ogonuki N, Miki H, Mochida K, Endoh K, Yomogida K, Fukumoto M, Horai R, Iwakura Y, Ito C, Toshimori K, Watanabe T, Satake M.
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Journal Title
Mol. Biol. Cell
Volume: 24
Issue: 17
Pages: 2633-44
DOI
Related Report
Peer Reviewed
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