The role of miR-210 on arterial remodeling by exxtravillous trophoblast
| Project/Area Number |
24592483
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Juntendo University (2013-2014)
Saitama Medical University (2012) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAJIHARA Takeshi 埼玉医科大学, 医学部, 准教授 (80286103)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 絨毛細胞 / マイクロRNA / らせん動脈 / ルシフェラーゼアッセイ / マイクロアレイ / リモデリング異常 / ケモカイン / 絨毛外栄養膜細胞 / HTR-8/SVneo / microRNA / 低酸素 / 浸潤能 / miRNA135b / ＣＸＣＬ１２ / microarray / 増殖能 / 網羅的解析 |
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| Outline of Final Research Achievements |
The expression of numerous miRNAs in the trophoblasts changes under low oxygen conditions. The aim of this study was to identify those miRNAs and their target genes associated with the trophoblast invasion under low oxygen conditions. Culturing the extravillous trophoblast (EVT) cell line, HTR-8/SVneo, at 2% oxygen as compared to 20% oxygen suppressed trophoblast invasion that correlated with increased expression of microRNA-135b (miR-135b) and decreased expression of the its predicted target gene CXCL12. Overexpression of miR-135b suppressed CXCL12 mRNA expression and invasion of HTR-8/SVneo cells. Adding a neutralizing antibody against CXCL12 to the culture medium suppressed HTR-8/SVneo cell invasion. Reporter assays showed that the 3'UTR sequence of CXCL12 was directly targeted by miR-135b. Our results suggest that miR-135b and CXCL12 play important roles in modulating the EVT invasion under low oxygen conditions.
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Report
(4 results)
Research Products
(5 results)
