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The role of miR-210 on arterial remodeling by exxtravillous trophoblast

Research Project

Project/Area Number 24592483
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionJuntendo University (2013-2014)
Saitama Medical University (2012)

Principal Investigator

ITAKURA Atsuo  順天堂大学, 医学部, 教授 (70262897)

Co-Investigator(Kenkyū-buntansha) KAJIHARA Takeshi  埼玉医科大学, 医学部, 准教授 (80286103)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords絨毛細胞 / マイクロRNA / らせん動脈 / ルシフェラーゼアッセイ / マイクロアレイ / リモデリング異常 / ケモカイン / 絨毛外栄養膜細胞 / HTR-8/SVneo / microRNA / 低酸素 / 浸潤能 / miRNA135b / CXCL12 / microarray / 増殖能 / 網羅的解析
Outline of Final Research Achievements

The expression of numerous miRNAs in the trophoblasts changes under low oxygen conditions. The aim of this study was to identify those miRNAs and their target genes associated with the trophoblast invasion under low oxygen conditions. Culturing the extravillous trophoblast (EVT) cell line, HTR-8/SVneo, at 2% oxygen as compared to 20% oxygen suppressed trophoblast invasion that correlated with increased expression of microRNA-135b (miR-135b) and decreased expression of the its predicted target gene CXCL12. Overexpression of miR-135b suppressed CXCL12 mRNA expression and invasion of HTR-8/SVneo cells. Adding a neutralizing antibody against CXCL12 to the culture medium suppressed HTR-8/SVneo cell invasion. Reporter assays showed that the 3'UTR sequence of CXCL12 was directly targeted by miR-135b. Our results suggest that miR-135b and CXCL12 play important roles in modulating the EVT invasion under low oxygen conditions.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2015 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Invited: 1 results)

  • [Journal Article] MicroRNA-135b suppresses extravillous trophoblast-derived HTR-8/SVneo cell invasion by directly down regulating CXCL12 under low oxygen conditions2015

    • Author(s)
      S Tamaru, Y Mizuno, H Tochigi, T Kajihara, Y Okazaki, R Okagaki, Y Kamei, O Ishihar, Itakura A
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 461 Issue: 2 Pages: 421-426

    • DOI

      10.1016/j.bbrc.2015.04.055

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Management of coagulopathy (obstetrical DIC).2014

    • Author(s)
      A. Itakura
    • Organizer
      Workshop on 46th International Congress on Pathophysiology of Pregnancy
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2014-09-18 – 2014-09-20
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] microRNA dependent CXCL12 expression under hypoxia controls invasion of extravillous trophoblast derived HTR-8/SVneo cells.2014

    • Author(s)
      S Tamaru, Y Mizuno, H Tochigi, H Kondo, T Kajihara, Y Okazaki, Y Kamei, O Ishihara, A Itakura
    • Organizer
      International Federation of Placenta Associations meeting 2014
    • Place of Presentation
      Paris, France
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 胎盤関連の分子 妊娠初期絨毛外栄養膜細胞において酸素濃度により発現変動するマイクロRNAとその標的遺伝子の網羅的解析と細胞浸潤能への関与2014

    • Author(s)
      田丸 俊輔, 板倉 敦夫, 梶原 健, 亀井 良政, 石原 理
    • Organizer
      第66回日本産科婦人科学会
    • Place of Presentation
      東京
    • Year and Date
      2014-04-18 – 2014-04-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 妊娠初期extravillous trophoblastの脱落膜浸潤に関わるマイクロRNAと標的遺伝子の網羅的解析2013

    • Author(s)
      田丸俊輔、 水野洋介、 梶原 健、 岡崎康司、 亀井良政、 石原 理、 板倉敦夫
    • Organizer
      第21回日本胎盤学会学術集会
    • Place of Presentation
      名古屋市 ウインクあいち
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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