The molecular mechanism of progesterone signaling to modulate cervical remodeling
| Project/Area Number |
24592488
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KATAYAMA Akira 日本医科大学, 医学部, 助教 (10333113)
NAKAI Akihito 日本医科大学, 医学部, 教授 (20227721)
KAWABATA Ikuno 日本医科大学, 医学部, 講師 (20328793)
TAKESHITA Toshiyuki 日本医科大学, 大学院医学研究科, 教授 (60188175)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | プロゲステロン / 早産予防 / 子宮頸管 / 頸管リモデリング / 頸管熟化 / 細胞外マトリクス |
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| Outline of Final Research Achievements |
Human uterine cervical fibroblast culture system was established from clinical specimens. Subsequently to the subculture in the medium containing beta-estradiol, expression dynamics of IL-6, IL-8, IL-1beta, PTGS2 and MMPs were assessed by real-time RT-PCR analysis.
LPS (2.0μg/ml) stimulation induced a significant increase of transcript levels of all molecules, which were significantly suppressed by progesterone (P4)(1.0μM）treatment except for IL-6, IL-8. On the other hand, the effect of stimulation with lower concentration of LPS (0.2μg/ml) was suppressed by P4 treatment in all molecules. Furthermore, the suppression was pronounced by the pretreatment of P4 1 hour before LPS stimulation.
Progesterone inhibit the expression of molecules related with preterm labor in a transcript level, and the timing of treatment is considered important in a clinical use to prevent preterm birth.
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Report
(4 results)
Research Products
(5 results)
