Expression and Functional Analysis of HOXD9 in Cervical Cancer

• Project/Area Number: 24592528
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: KATSUMI Tsukazaki 慶應義塾大学, 医学部, 准教授 (40118972)
• Co-Investigator(Kenkyū-buntansha): 岩田 卓 慶應義塾大学, 医学部, 専任講師 (30296652) ; 谷口 智憲 慶應義塾大学, 医学部, 助教 (40424163)
• Co-Investigator(Renkei-kenkyūsha): 河上 裕 慶應義塾大学, 医学部, 教授 (50161287)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 子宮頸癌 / HOXD9

Outline of Final Research Achievements

We analyzed the expression and function of HOXD9 in cervical cancers. As a result, HOXD9 gene was overexpressed in cervical cancer tissues than normal cervical tissues. Clinicopathogical analysis using 51 cervical cancer cases revealed HOXD9 expression was associated with lymphovascular space invasion (LVSI) and lymph node metastasis. Silencing of the HOXD9 gene inhibited cell proliferation of cervical cancer cell lines. These results suggested HOXD9 gene is overexpressed in high risk cervical cancer cases and plays important role of cervical cancer cell growth. It has been shown that activation of tumor P53 has occurred by the suppression of HOXD9. HOXD9 is associated with malignant potential in cervical cancer and may be a novel potential target of cervical cancer therapy.