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A new treatment strategy for corneal and choroidal neovascularization through TRP channel

Research Project

Project/Area Number 24592638
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Ophthalmology
Research InstitutionWakayama Medical University

Principal Investigator

OKADA Yuka  和歌山県立医科大学, 医学部, 講師 (50264891)

Co-Investigator(Kenkyū-buntansha) SAIKA Shizuya  和歌山県立医科大学, 医学部, 教授 (40254544)
SHIRAI Kumi  和歌山県立医科大学, 医学部, 講師 (70326370)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords血管新生 / 角膜 / 脈絡膜 / TRPチャネル / 黄斑変性 / TRP
Outline of Final Research Achievements

Corneal stromal neovascularization from the limbal vessels was induced by cauterization of the central cornea of an eye of TRPV1 KO, TRPA1 KO or WT mice by disposable cauterization tool. We then examined the length of neovascularization from limbus toward the center of the cornea following cauterization in histological section with a monoclonal anti-CD31 antibody. mRNA expression of VEGF and TGFβ1 in a mouse cornea was suppressed by the loss of TRPV1. TRPV1 KO, TRPA1 KO, TRPV4 KO or WT were included, and one eye of each was used.Retinas received laser photocoagulation for the induction of experimental CNV under slit lamp microscope observation and laser photocoagulator. A coverslip was applied to the cornea to view the retina with sodium hyaluronate. Four lesions were produced using a power of 100 mW, a spot size of 100 μm, and a duration of 0.2s. These TRP KO mice are suppressed CNV after photocoagulation.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (4 results)

All 2014 2013 Other

All Presentation (4 results)

  • [Presentation] Role of TRPA cation channel receptor signal in development of stromal neovascularization in a mouse cornea2014

    • Author(s)
      Kusumoto K, Okada Y, Tomoyose K, Miyajima M, Reinach PS, Saika S
    • Organizer
      ARVO
    • Place of Presentation
      デンバー アメリカ
    • Year and Date
      2014-05-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] マウス角膜での血管新生におけるTRPV1の役割2013

    • Author(s)
      友寄勝夫 岡田由香 住岡孝吉 藤田識人 白井久美 雑賀司珠也
    • Organizer
      日本眼科学会総会
    • Place of Presentation
      東京フォーラム
    • Related Report
      2013 Research-status Report
  • [Presentation] Impaired angiogenic response in cornea by lacking TRPV1 in mice2013

    • Author(s)
      Katsuo Tomoyose, Yuka Okada, Takayoshi Sumioka, Kumi Shirai and Shizuya Saika
    • Organizer
      The Association for Research in Vision and Opyjalmology
    • Place of Presentation
      Seattle. USA
    • Related Report
      2013 Research-status Report
  • [Presentation] マウス角膜での血管新生におけるTRPV1の役割

    • Author(s)
      友寄勝夫、岡田由香、住岡孝吉、藤田識人、白井久美、雑賀司珠也
    • Organizer
      日本眼科学会総会
    • Place of Presentation
      東京フォーラム
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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