A new treatment strategy for corneal and choroidal neovascularization through TRP channel

• Project/Area Number: 24592638
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Wakayama Medical University
• Principal Investigator: OKADA Yuka 和歌山県立医科大学, 医学部, 講師 (50264891)
• Co-Investigator(Kenkyū-buntansha): SAIKA Shizuya 和歌山県立医科大学, 医学部, 教授 (40254544) ; SHIRAI Kumi 和歌山県立医科大学, 医学部, 講師 (70326370)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 血管新生 / 角膜 / 脈絡膜 / TRPチャネル / 黄斑変性 / TRP

Outline of Final Research Achievements

Corneal stromal neovascularization from the limbal vessels was induced by cauterization of the central cornea of an eye of TRPV1 KO, TRPA1 KO or WT mice by disposable cauterization tool. We then examined the length of neovascularization from limbus toward the center of the cornea following cauterization in histological section with a monoclonal anti-CD31 antibody. mRNA expression of VEGF and TGFβ1 in a mouse cornea was suppressed by the loss of TRPV1. TRPV1 KO, TRPA1 KO, TRPV4 KO or WT were included, and one eye of each was used.Retinas received laser photocoagulation for the induction of experimental CNV under slit lamp microscope observation and laser photocoagulator. A coverslip was applied to the cornea to view the retina with sodium hyaluronate. Four lesions were produced using a power of 100 mW, a spot size of 100 μm, and a duration of 0.2s. These TRP KO mice are suppressed CNV after photocoagulation.

Research Products

• [Presentation] Role of TRPA cation channel receptor signal in development of stromal neovascularization in a mouse cornea (2014)
  • Author(s): Kusumoto K, Okada Y, Tomoyose K, Miyajima M, Reinach PS, Saika S
  • Organizer: ARVO
  • Place of Presentation: デンバー アメリカ
  • Year and Date: 2014-05-03
• [Presentation] マウス角膜での血管新生におけるTRPV1の役割 (2013)
  • Author(s): 友寄勝夫 岡田由香 住岡孝吉 藤田識人 白井久美 雑賀司珠也
  • Organizer: 日本眼科学会総会
  • Place of Presentation: 東京フォーラム
• [Presentation] Impaired angiogenic response in cornea by lacking TRPV1 in mice (2013)
  • Author(s): Katsuo Tomoyose, Yuka Okada, Takayoshi Sumioka, Kumi Shirai and Shizuya Saika
  • Organizer: The Association for Research in Vision and Opyjalmology
  • Place of Presentation: Seattle. USA
• [Presentation] マウス角膜での血管新生におけるTRPV1の役割
  • Author(s): 友寄勝夫、岡田由香、住岡孝吉、藤田識人、白井久美、雑賀司珠也
  • Organizer: 日本眼科学会総会
  • Place of Presentation: 東京フォーラム